“…These methods include grey-scale histogram analysis, [14][15][16][17][18][19] texture analysis, 20,21 edge enhancement and linking, 16,[20][21][22][23][24][25] region growing, 20,26,27 contour following, 16,21,23,25,28 and the maximisation of spatial correlation or mutual information between images. 18,[29][30][31][32][33][34][35][36][37] Landmark-based methods rely on marking and aligning easily identifiable point features, 15,18,38,39 and manual landmarking is one of the main methods used in the commercial proteome software. Segmentation methods divide the picture up into areas or 'segments', often by contour following, or by region growing and the related watershed methods, [40][41][42][43][44] which can then be moved for image alignment.…”