Patients in whom active tuberculosis was diagnosed after admission to a Japanese university hospital from 2005 through 2007

Fukushima, Yasutsugu; Shiobara, Kanae; Shiobara, Taichi; Tatewaki, Masamitsu; Anzai, Makiko; Fukushima, Fumiya; Yamada, Issei; Hirata, Hirokuni; Sugiyama, Kumiya; Fukuda, Takeshi

doi:10.1007/s10156-011-0239-9

Cited by 6 publications

(11 citation statements)

References 11 publications

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“…Fukushima and collaborators in Japan [47] propose that tuberculosis should be suspected in any patient with respiratory symptoms. Sputum tests for acid-fast bacteria should be performed at least three times initially.…”

Section: Discussionmentioning

confidence: 99%

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Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples

Rodriguez-Nuñez

Avelar

Márquez³

et al. 2011

J Infect Dev Ctries

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Introduction: Lymph node tuberculosis (TB) is the leading cause of extrapulmonary tuberculosis and is the most frequently identified type in Aguascalientes, Mexico. Conventional diagnosis has serious limitations for rapid detection of extrapulmonary tuberculosis in clinical samples. Here PCR and modified FISH have been tested as complementary diagnosis methods for extrapulmonary tuberculosis. Methodology: The specific insertion sequence IS6110 for Mycobacterium tuberculosis complex was used to perform PCR and build DNA and PNA FISH probes (20bp). PCR and modified DNA and PNA FISH assays were performed to evaluate 41 lymph node paraffin-embedded tissue samples, in comparison with the histopathology diagnosis, which was considered the gold standard (22 positive and 19 negative). Results: In comparison with histopathology diagnosis PCR showed 62.5 % sensitivity and 77.8 % specificity (χ 2 = 4.583 p< 0.05). Modified DNA FISH showed 71.4% sensitivity and 84.6% specificity (χ 2 = 11.21 p< 0.05). PNA FISH showed 66.7% sensitivity and 60.0% specificity (χ 2 = 2.93 p> 0.05). Ziehl Neelsen stain was positive in only four cases of 22 lymph node samples positive to histopathology. In contrast, PCR and modified DNA FISH were positive in 20 cases of the same group. The negative cases were coincident in all tests. Conclusions: PCR and DNA FISH showed a significant increase in the number of cases detected and also showed higher sensitivity and specificity compared with data reported by traditional methodology. In developing countries, these techniques could help to complement the early diagnosis and timely treatment of extrapulmonary tuberculosis.

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Section: Discussionmentioning

confidence: 99%

“…Our results showed higher sensitivity and specificity in the detection of MTC in comparison to ZN staining, PCR, and modified FISH. However, while these oligonucleotides can distinguish between NTM and MTC, they cannot distinguish between Mycobacterium tuberculosis and BCG, because BCG is part of MTC [47].…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples

Rodriguez-Nuñez

Avelar

Márquez³

et al. 2011

J Infect Dev Ctries

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“…The increasing ratio of elderly patients with newly diagnosed tuberculosis is a major medical and healthcare issue in Japan (6,7). The incidence of tuberculosis infection was frequent in Japan prior to the 1960s, and the aging of these infected individuals is one of the reasons for the relatively high prevalence in Japan at present (7)(8)(9)11). Elderly patients tend not to present with typical symptoms, such as weight loss, coughing and fever, and may not be able to expectorate sputum spontaneously, resulting in a delayed diagnosis (6,7,9,12,13).…”

Section: Discussionmentioning

confidence: 99%

“…The incidence of tuberculosis infection was frequent in Japan prior to the 1960s, and the aging of these infected individuals is one of the reasons for the relatively high prevalence in Japan at present (7)(8)(9)11). Elderly patients tend not to present with typical symptoms, such as weight loss, coughing and fever, and may not be able to expectorate sputum spontaneously, resulting in a delayed diagnosis (6,7,9,12,13). In addition, as the life expectancy of the Japanese population increases, the increasing incidence of aspiration pneumonia in elderly patients emerges as a key medical and healthcare concern (1,2,4,5,14,15).…”

Section: Discussionmentioning

confidence: 99%

“…Although the prevalence of tuberculosis was subsequently reduced to 16.7 per 100,000 of the Japanese population in 2012, this figure remains 3-4 times higher than the prevalence of tuberculosis in Europe and North America (8). In Japan, one reason for the relatively high prevalence of tuberculosis is the aging of individuals who were previously infected with the disease (9).…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic delay of pulmonary tuberculosis in patients with acute respiratory distress syndrome associated with aspiration pneumonia: Two case reports and a mini-review from Japan

Nakao¹,

Sone²,

Kagawa³

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Diagnosing active tuberculosis in elderly patients presents problems due to nonspecific symptoms and complications such as aspiration pneumonia. The current study presents two cases of pulmonary tuberculosis with bilateral pulmonary infiltrates associated with aspiration pneumonia. The two elderly patients developed acute respiratory distress syndrome as a result of aspiration pneumonia. The diagnoses of pulmonary tuberculosis were delayed in both cases, as the patients were diagnosed with active tuberculosis following discharge from hospital. The sputum test for acid-fast bacillus at the time of administration was smear-negative/culture-positive in these patients. They were treated with isoniazid, rifampicin and ethambutol, and nosocomial transmission of tubercu losis from these patients was not reported. The number of elderly patients with aspiration pneumonia is predicted to increase rapidly, and aspiration pneumonia combined with pulmonary tuberculosis is a major medical and healthcare concern in Japan. The present study concludes that physicians should always consider the complication of pulmonary tuberculosis when treating pneumonia patients, in particular in treating elderly patients with pulmonary infiltrates.

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Immunonutritional status and pulmonary cavitation in patients with tuberculosis: A revisit with an assessment of neutrophil/lymphocyte ratio

Nakao¹,

Muramatsu²,

Arakawa³

et al. 2019

Respiratory Investigation

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Patients in whom active tuberculosis was diagnosed after admission to a Japanese university hospital from 2005 through 2007

Cited by 6 publications

References 11 publications

Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples

Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples

Diagnostic delay of pulmonary tuberculosis in patients with acute respiratory distress syndrome associated with aspiration pneumonia: Two case reports and a mini-review from Japan

Immunonutritional status and pulmonary cavitation in patients with tuberculosis: A revisit with an assessment of neutrophil/lymphocyte ratio

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