The aim of this study was the development
and characterization
of a new microemulsion composed of sweet orange (Citrus sinensis L.) essential oils (EOCs) and evaluate its antibacterial and larvicide
profiles. The optimal combination of EOCs, surfactant (Kolliphor EL),
and water was achieved using a ternary phase diagram. EOCs were obtained
from orange peel by steam-distillation and analyzed by gas chromatograph–mass
spectrometer (GC-MS). A total of 25 compounds were identified, among
which 90 wt % was d-limonene. In order to determine antimicrobial
activity of the formulation, minimum inhibitory concentration (MIC)
assay was done. d-Limonene, limonene 1,2-epoxide, and β-mircene
showed a high antibacterial activity. Cell viability studies were
performed in L929 murine fibroblast as a cellular line, using the
MTT assay. A cell viability of 118% for EOCs and 117.7% for microemulsion
after 72 h was recorded indicating no relevant cytotoxic effect. In vivo studies, based on a wax moth, Galleria mellonella (G. mellonella), larvae as a model system showed
100% of larvae mortality under the influence of the microemulsion
with EOCs, while a second efficiency rating of 45% for larvae mortality
was reached for pure EOCs. These tests confirm a strong larvicide
activity for the essential oil, characterized by the highest concentration
of limonene as the larvicide agent. The loading of EOCs into microemulsions
offered an improved approach for the delivery of this oil which retained
its bioactivity, as shown in vitro and in vivo.