Pattern of Albumin, Immunoglobulins, and Glucose in Cerebrospinal Fluid and Serum of Patients with Disorders of the Central Nervous System

Osenbrück, Margret; Rao, Marie Luise; Quednau, H. D.

doi:10.1159/000115756

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Our IgG levels in CSF from non-MS controls are similar to those found by competitive enzyme immunoassay (8). Although the latter did not quantitate IgG or albumin levels in MS CSF, our values for IgG and albumincorrelate well with those reported by nephlometry (15).…”

Section: Discussionsupporting

confidence: 90%

Quantitation of IgG and albumin in CSF and serum from multiple sclerosis patients by enzyme-linked immunosorbent assay

Mehta

1987

Acta Neurologica Scandinavica

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Immunoglobulin G (IgG) and albumin from unconcentrated cerebrospinal fluid (CSF) and serum of patients with multiple sclerosis (MS) and non-MS controls were quantitated using enzyme-linked immunosorbent assay (ELISA). The IgG levels, IgG/albumin ratios and IgG indexes were significantly increased in CSF of MS patients compared to those of non-MS controls. The method is sensitive, rapid and reproducible and can be applied to routine laboratory use for quantitation of IgG and albumin in unconcentrated CSF from humans as well as in experimental animals used as models for demyelinating diseases.

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Section: Discussionsupporting

confidence: 90%

Quantitation of IgG and albumin in CSF and serum from multiple sclerosis patients by enzyme-linked immunosorbent assay

Mehta

1987

Acta Neurologica Scandinavica

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“…They based this proposal on the results of a small study (34 patients with cerebral infarction, 10 of whom had blood-CSF barrier damage), in which they found a relatively higher increase in CSF/S IgG ratio than in CSF/S albumin ratio with increasing val ues for the CSF/S albumin ratio. Other studies have not found such a relative increase in CSF/S IgG ratio in patients with cerebrovascular disease [14]. In the present study, the relation between the CSF/S albumin ratio and the CSF/S IgG ratio was linear (and almost identical) …”

Section: Resultssupporting

confidence: 72%

Protein Analysis in Cerebrospinal Fluid

et al. 1993

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To examine the possible influence of the blood-CSF barrier function on mathematical formulas for estimating intrathecal IgG production {IgG index, Tourtellotte’s formula [TOURT], Schuller and Sagar’s formula [Schull], and Reiber and Felgenhauer’s formula [IgG(loc)]}, we analysed serum and CSF samples from 105 healthy individuals, aged 18-88 years, and 816 consecutive patients without oligoclonal IgG bands (OCBs) in the CSF, aged 18-91 years. In healthy individuals there was no significant correlation between the CSF/serum (S) albumin ratio (i.e. blood-CSF barrier function) and the IgG index, a dimensionless quotient for determining intrathecal IgG production, and only a small correlation (r = –0.08, p < 0.05) in patients without OCBs. However, significant dependence on the blood-CSF barrier function was found, in both healthy individuals and patients without OCBs, for the formulas determining intrathecal IgG production in milligrams (per litre CSF or per day), i.e. for TOURT, SCHULL and IgG(loc). Therefore, we propose a new formula for the quantitative determination of intrathecal IgG production in mg/l, the IGGPROD, which is calculated as: CSF-IgG - [(0.51 X CSF-Alb X S-IgG)/ S-Alb]. IGGPROD is an empirical formula, based on findings in 105 healthy individuals 18-88 years of age, and does not depend on the blood CSF barrier function.

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“…Regarding the protein electrophoretic patterns of dogs affected by chronic canine distemper, the elevation in gamma-globulin fractions is a consequence of an increased intrathecal synthesis of immunoglobulins, as described in several disorders in human beings such as multiple sclerosis and subacute sclerosing panencephalitis ( OSENBRUCK et al, 1985;SORJONEN, 1987).…”

Section: Discussionmentioning

confidence: 99%

Cytological, Immunocytochemical and Biochemical Cerebrospinal Fluid Investigations in Selected Central Nervous System Disorders of Dogs

Abate

Bollo

Lotti

et al. 1998

Journal of Veterinary Medicine, Series B

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Cerebrospinal fluid (CSF) obtained from 20 clinically healthy dogs and from 15 dogs affected by neurological disorders were examined for total and differential cell counts, immunocytochemistry for canine distemper virus antigen, total protein concentration and electrophoretic separation, and glucose and enzyme determination. Dogs affected by canine distemper showed an increase in macrophages, presence of specific inclusion bodies, and an increase in total protein concentration and gamma-globulin fraction. In bacterial meningoencephalomyelitis pleocytosis, increase of total proteins with special regard to high molecular weight proteins, elevated levels of lactate dehydrogenase (LDH), alterations of LDH isoenzyme profile, and decrease in glucose levels were detected; inflammatory disorders were more often characterized by an increase in LDH level, while in non-inflammatory disorders (hydrocephalus and spinal cord neoplasia) no variation in LDH levels was detected. Analysis of CSF in dogs appears relatively easy to perform and may help in establishing the condition of the blood-brain barrier as well as in the diagnosis of neurological disorders.

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Pattern of Albumin, Immunoglobulins, and Glucose in Cerebrospinal Fluid and Serum of Patients with Disorders of the Central Nervous System

Cited by 5 publications

References 4 publications

Quantitation of IgG and albumin in CSF and serum from multiple sclerosis patients by enzyme-linked immunosorbent assay

Quantitation of IgG and albumin in CSF and serum from multiple sclerosis patients by enzyme-linked immunosorbent assay

Protein Analysis in Cerebrospinal Fluid

Cytological, Immunocytochemical and Biochemical Cerebrospinal Fluid Investigations in Selected Central Nervous System Disorders of Dogs

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