Pattern of AST and ALT changes in Relation to Hemolysis in sickle cell Disease

Nsiah, Kwabena; Dzogbefia, V.P.; Ansong, Daniel; Osei‐Akoto, Alex; Boateng, Henry A.; Ocloo, D

doi:10.4137/cmbd.s3969

Cited by 25 publications

(25 citation statements)

References 28 publications

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“…Elevated aminotransferase levels are commonly associated with compromised hepatic integrity from various pathophysiology (Banerjee et al, 2001). Elevated AST and ALT in this study could be due to the liver involvement in the pathophysiology of sickle cell diseases/crisis due to the fact that AST and ALT are liver enzymes that could leak to the plasma in hepatic disorder This finding is also consistent with the report of Nsiah et al,2011 that in HbSS and HbSC, aspartate transaminase (AST) is released via intravascular hemolysis. In addition there used to be massive haemolysis in sickle cell crisis making the liver to metabolise RBC excessively which may bring about liver disorder hence the biochemical alterations in the value AST and ALT (Banerjee et al, 2001;Nsiah et al,2011) Zn and Se are antioxidants and acute phase metals.…”

Section: Discussionsupporting

confidence: 89%

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Investigation of Some Biochemical Parameters in Subject with HbSC and HbSS in Saki West Local Government - a Rural Community in Nigeria

Olaniyan¹

2016

Am. J. Biomed. Sci.

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Background: HbSS and HbSC are a group of genetic blood disorders. They are examples of sickle cell diseases.The complication of sickle cell disease could affect spleen, brain, eyes, lungs, liver, heart, kidneys, penis, joints, bones, or skin. Aim and Objective: This work was designed to measure plasma ALT, AST, Albumin,Se, Zn and Uric acid in HbSS and HbSC patients in Saki-West Local Government area of Oyo state-Nigeria. Materials and Methods: HbSS and HbSC patients in crisis and in non-crisis steady state classified into HbSC (n=25) and HbSS (n=25) aged 10 to 31 years were recruited as test subjects for the study. Apparently healthy 50 HbAA volunteers classified into female (n=25) and male (n=25) aged 10 -50 years were also studied as control. Subjects residing in Saki-West for not less than 5 years were recruited as test and control subjects. Plasma Albumin, Uric acid, ALT(Alanine Transaminase), AST(Aspartate Trans aminase) were determined biochemically bt spectrophotometry.Plasma Zn (Zinc) and Se (Selenium) were measured by Atomic Absorption Spectrophotometry. Results: The result obtained showed a significantly higher mean value of plasma ALT and AST in HbSC and HbSS than HbAA with p<0.05. The result also showed a significantly lower plasma selenium mean value in HbSC and HbSS than HbAA with p<0.05 This biochemical alterations including plasma Zn was more in crisis than in non-crisis steady state. Conclusion and Recommendation: Decreased plasma level of Selenium and increased plasma AST and ALT were found in HbSS and HbSC patients. This biochemical alterations including plasma Zn was more in crisis than in non-crisis steady state.

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Section: Discussionsupporting

confidence: 89%

“…Elevated aminotransferase levels are commonly associated with compromised hepatic integrity from various pathophysiology. In HbSS and HbSC, aspartate transaminase (AST) is released via intravascular hemolysis (Nsiah et al 2011). Serum creatinine may be associated with renal insufficiency in sickle cell patients.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Some Biochemical Parameters in Subject with HbSC and HbSS in Saki West Local Government - a Rural Community in Nigeria

Olaniyan¹

2016

Am. J. Biomed. Sci.

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“…Elevated aminotransferase levels are commonly associated with compromised hepatic integrity from various pathophysiology. In SCD, aspartate transaminase (AST) is released via intravascular hemolysis [11]. Serum creatinine may be associated with renal insufficiency in sickle cell patients.…”

Section: Introductionmentioning

confidence: 99%

“…AST:ALT (Alanine Aminotransferase) ratio may play role as a hemolytic marker, because it has an inverse association with the hemoglobin level. Whether in steady state or in crisis, provided hepatic and cardiac integrity has not been compromised, subjects with SCD would have higher AST levels due to the hemolytic nature of the condition [11]. Plasma hexose sugar levels and metabolism are altered in SCD.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Indicator of Sickle Cell Disease: Preliminary Report from India

et al. 2011

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Blood biochemistry has significant effect on pathophysiology of human body. Recently few studies found the association of biochemical abnormalities in sickle cell patients. Sickle cell disease showed clinical variability where African ancestors have severe phenotype than Indian sicklers. Our aim was to evaluate the biochemicals in sickle cell patients and their effect on severity. Here we present the comparative biochemical levels in sickle cell patients as well as controls. Sickle cell patients diagnosed by HPLC and biochemical analysis done by Beckman-auto analyzer. T test applied for statistical analysis. Result showed the renal abnormality lesser in patients and related biochemical within the normal range and statistically not significant. Electrolytes, hepatic enzymes, alkaline phosphatase and glucose were elevated and statistically significant (P value \0.05). Observation of the study concludes the biochemical abnormality play a significant role in sickle cell patient's physiopathology and can be used to management of the disease.

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“…12 Increased lactate concentration after 72 hours may also have interfered with the methodology. Creatinine increased by 71.4% after 72 hours and by 185% after 3 months (Graph 1), which was attributable to interference of pseudocreatinines as shown by Heins et al 13,14 Such massive increase cannot be explained just by hemoconcentration. Potassium concentration though showed increase after 24 hours, significant increase of 50% was seen only after 72 hours.…”

Section: Resultsmentioning

confidence: 81%

Does Prolonged Storage of Serum Samples alter the Lab Results?

Tayal¹,

Gupta²

2017

Indian Journal of Medical Biochemistry

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Background Sample storage for prolonged periods can lead to alterations in routine biochemical parameters. Aim This study was undertaken to observe the biochemical changes on serum samples when they are stored for prolonged periods at –20°C on different routine biochemical parameters. Results Significant changes were observed in serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and potassium levels after 72 hours of storage, which further deteriorate in 3 months. Rest of the parameters did not show any significant alteration. Conclusion Prolonged storage of samples lead to alterations in the analyte concentration in serum. Serum glucose, AST, ALT, creatinine, and potassium levels should be estimated within 72 hours if prolonged storage is unavoidable. In conclusion, all the parameters should be assayed within 24 hours to avoid misinterpretation of results and better patient care. How to cite this article Tayal D, Gupta M, Goswami B. Does Prolonged Storage of Serum Samples alter the Lab Results? Indian J Med Biochem 2017;21(1):30-33.

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Pattern of AST and ALT changes in Relation to Hemolysis in sickle cell Disease

Cited by 25 publications

References 28 publications

Investigation of Some Biochemical Parameters in Subject with HbSC and HbSS in Saki West Local Government - a Rural Community in Nigeria

Investigation of Some Biochemical Parameters in Subject with HbSC and HbSS in Saki West Local Government - a Rural Community in Nigeria

Biochemical Indicator of Sickle Cell Disease: Preliminary Report from India

Does Prolonged Storage of Serum Samples alter the Lab Results?

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