Pattern of circulating microparticles in chronic heart failure patients with metabolic syndrome: Relevance to neurohumoral and inflammatory activation

Abstract

BackgroundThe role of pattern of circulating endothelial cell-, platelet-, and monocyte-derived microparticles in metabolic syndrome (MetS) patients with chronic heart failure (CHF) is not still understood.The aim of the study was to investigate a pattern of circulating MPs in MetS patients with CHF in relation to neurohumoral and inflammatory activation.MethodsThe study retrospectively involved 101 patients with MetS (54 subjects with CHF and 47 patients without CHF) without documented coronary artery stenosis > 50% at least of one artery and 35 healthy volunteers. Biomarkers were measured at baseline of the study. Circulating MPs were phenotyped by flow cytometry technique.ResultsThe results of the study have shown that numerous of the circulating platelet-derived and monocyte-derived MPs in subjects with MetS (with or without CHF) were insufficiently distinguished from the level obtained in healthy volunteers. We found an elevated level of CD31 +/annexin V + MPs in association with a lower level of CD62E + MPs. All these led to decreased CD62E + to CD31 +/annexin V + ratio among patients with MetS in comparison with healthy volunteers, as well as in MetS patients with CHF compared with those who did not demonstrated CHF. Therefore, we found that biomarkers of biomechanical stress (NT-proBNP) and inflammation (hs-CRP, osteoprotegerin) remain statistically significant predictors for decreased CD62E + to CD31 +/annexin V + ratio in MetS patients with CHF.In conclusion, decreased CD62E + to CD31 +/annexin V + ratio reflected impaired immune phenotype of MPs may be discuss surrogate marker of CHF development in MetS population.

Up-to-date clinical approaches of biomarkers’ use in heart failure

“…Moreover, increased number of apoptotic EMPs and decreased number of EPCs in circulation has found as powerful predictor of CV death, HF-related admission to the hospital and prognosticator of positive reply to medical therapy in short-term period (Berezin, 2015c;Berezin et al, 2015a;. There is novel HF risk prediction score created by means of biomarkers including NPs, galectin-3, high sensitive CRP and estimated ratio between both numbers of apoptotic EMPs and EPCs (Berezin, 2015c).…”

Progenitor Cell Dysfunction: The Role of Endothelial Precursors in Heart Failure

“…On the other hand, EPCs that circulate in the peripheral blood in patients at higher risk of HF and in individuals with established HF presented lowered survival ability and partially inconvenience to be differentiated to mature endothelial cells under influence of essential innate stimuli including transforming growth factor-beta, tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines [15,16]. This phenomenon was previously described as EPC dysfunction and it was widely diagnosed in the patients with diabetes, abdominal obesity, hypertension and other CV risk factors [17].…”

Are Epigenetic Features Essential in Advance of Heart Failure Phenotypes?

“…Altered miRNA signature risk of CV death [32][33][34][35][36][37] . Whether interactions between features of transcriptomics, proteomics and metabolomics are specifically for HFrEF and HFpEF remains still not clear.…”