“…Nevertheless, although most neurons exhibited those markers, few showed important functional readouts, such as TRPV1 activation, as only about 1-2% of the cells responded to capsaicin. Other studies were able to demonstrate the expression of canonical peripheral markers, such as BRN3A, peripherin and b-Tubulin III, in sensory neurons derived from hiPSCs, but evidence of specific functional activity was still missing (Menendez et al, 2013, Young et al, 2014, Blanchard et al, 2015, Eberhardt et al, 2015. Young et al (2014) reported measuring TRPV1 activity induced by capsaicin, but only after 6 weeks in medium containing growth factors.…”