Pattern of ischemia reperfusion injury in a mouse orthotopic liver transplant model

Que, Xingyi; Debonera, Fotini; Xie, Jinfu; Furth, Emma E.; Aldeguer, Xavier; Gelman, Andrew E.; Olthoff, Kim M.

doi:10.1016/j.jss.2003.07.005

Cited by 36 publications

(28 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their induction rapidly increased with prolongation of cold preservation time and was negatively correlated with graft size. Interestingly, neither IP-10 nor TCA3 showed significant induction in previous studies involving either warm liver ischemia/reperfusion or whole liver transplantation with minimum cold preservation (17,30,31). We found that both chemokines were induced within four hr and declined to basal levels by 24 hr posttransplantation, suggesting the importance of early neutralization of chemokine function.…”

Section: Discussioncontrasting

confidence: 67%

“…However, models of hepatocellular injury due to warm ischemia are less relevant to clinical liver transplantation, leading us to utilize a mouse liver transplant model involving cold preservation and transplantation of partial grafts (17). Using this model, we now showed that IP-10 and TCA3 were the two most highly induced chemokines.…”

Section: Discussionmentioning

confidence: 99%

“…These techniques expanded the donor pool size and have helped reduce death on the waiting list for a liver transplant (26). However, although various clinical studies of partial liver transplants have been conducted (27)(28)(29), animal models remain critical to the ongoing investigation of the molecular mechanisms of liver injury and regeneration following partial liver transplantation (17,30,31). Compared to whole grafts, partial grafts have the additional burden of undergoing regeneration while recovering from cold preservation injury, a task that can be overwhelming and lead to poor outcome (5).…”

Section: Discussionmentioning

confidence: 99%

“…Syngeneic orthotopic liver transplants were performed under isoflurane inhalational anesthesia using a commercial system (Surgivet) and a surgical microscope by modification of published techniques (14 -16), as described previously (17). Briefly, 50% grafts were prepared by removal of the left lateral lobe, caudate lobe, and left medium lobe, and the remainder of the procedure was done as for whole graft transplantation.…”

Section: Surgery and Study Designmentioning

confidence: 99%

See 3 more Smart Citations

Selective Neutralization of the Chemokine TCA3 Reduces the Increased Injury of Partial Versus Whole Liver Transplants Induced by Cold Preservation

Xie

Wang²,

Debonera³

et al. 2006

Transplantation

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Surgery and Study Designmentioning

confidence: 99%

See 2 more Smart Citations

Selective Neutralization of the Chemokine TCA3 Reduces the Increased Injury of Partial Versus Whole Liver Transplants Induced by Cold Preservation

Xie

Wang²,

Debonera³

et al. 2006

Transplantation

Self Cite

View full text Add to dashboard Cite

show abstract

“…In several new studies, mainly in mouse models, IL-6 appears to be necessary in the early reperfusion period for liver cell regeneration. 38,39 …”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Early CD4+ T Cell Reduction in Hepatic Ischemia/Reperfusion Injury

Martin

Mory

Prescher

et al. 2010

Journal of Gastrointestinal Surgery

View full text Add to dashboard Cite

show abstract

Stat3‐Atg5 signal axis inducing autophagy to alleviate hepatic ischemia‐reperfusion injury

Han

Zhao

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

In performing our experiment, impaired autophagy increased hepatocellular damage during the reperfusion period. It was demonstrated by the effect of blocking autophagy using bafilomycin A1 or knocking Atg5 gene out reduces the anti-apoptotic effect of Stat3. Here we focus on the role of signal transducer and activator of transcription 3 (Stat3) in regulating autophagy to alleviate hepatic IRI. We found that Stat3 was up-regulated during hepatic IRI and was associated with an activation of the autophagic signaling pathway. This increased Stat3 expression, which was allied with high autophagic activity, alleviated liver damage to IR, an effect which was abrogated by Stat3 epletion as demonstrated in both in vivo and in vitro methods. The levels of Atg5 protein were decreased when Stat3 was inhibited by HO 3867 or siStat3. We conclude that Stat3 appeared to exert a pivotal role in hepatic IRI, by activating autophagy to alleviate hepatic IRI, and Atg5 was required for this process. The identification of this novel pathway, that links expression levels of Stat3 with Atg5-mediated autophagy, may provide new insights for the generation of novel protective therapies directed against hepatic IRI.

show abstract

Pattern of ischemia reperfusion injury in a mouse orthotopic liver transplant model

Cited by 36 publications

References 23 publications

Selective Neutralization of the Chemokine TCA3 Reduces the Increased Injury of Partial Versus Whole Liver Transplants Induced by Cold Preservation

Selective Neutralization of the Chemokine TCA3 Reduces the Increased Injury of Partial Versus Whole Liver Transplants Induced by Cold Preservation

Protective Effects of Early CD4+ T Cell Reduction in Hepatic Ischemia/Reperfusion Injury

Stat3‐Atg5 signal axis inducing autophagy to alleviate hepatic ischemia‐reperfusion injury

Contact Info

Product

Resources

About