Pattern of Notch2, Numb, and Cas8 Gene Expression in Relevant Structures of the Rat Brain during Formation of Spatial Memory

Abstract

The expression of Notch2, Numb, and Cas8 genes, whose protein products are involved in regulation of neurogenesis/neuroapoptosis processes, was studied in the relevant cerebral structures of male Wistar rats trained in a spatial habit. The formation of long-term spatial memory was found to be associated with the formation of a specific pattern of transcription activity of the studied genes in different brain structures. The maximum expression of Notch2 gene was found in the hippocampus and cerebellum, the maximum expression of Numb was detected in the prefrontal cortex and cerebellum, and the maximum expression of Cas8 was revealed in the prefrontal cortex of trained animals.

Effects of Antibodies to Glutamate on Cerebral Expression of the Tnfrsf1A Gene under Conditions of Spatial Amnesia Induced by Proinflammatory Protein S100A9 Fibrils in Aging Mice

“…In 24 h after the end of behavioral experiments, all animals were decapitated, the hippocampus, pre-frontal cortex, and cerebellum were isolated at 4°C. The expression of the Tnfrsf1A gene in samples of the brain structures was measured by real-time PCR according to a previously described protocol [11]. Tnfrsf1A expression in the cerebral structures of agematched native mice served as the control level when calculating the expression level in the control and experimental groups [12].…”

Post-Developmental Roles of Notch Signaling in the Nervous System

“…Furthermore, postnatal knockout of Rbpj [ 66 ] and Adam10 [ 67 ] in the brain using CamKII-Cre have also been shown to exhibit defects in learning and memory. Additionally, a study in rats showed that Notch2 is expressed in the hippocampus and cerebellum, where its expression becomes significantly upregulated upon establishment of long-term spatial memory [ 68 ]. Although these parallels suggest that Notch signaling may have already been utilized for primitive forms of learning and memory in the urbilaterian, the last common ancestor of invertebrate and vertebrate species [ 69 ], several differences found between fly and mammalian systems are worth mentioning.…”