Pattern Recognition of Tobacco Headspace GC Profiles: A Potential New Analytical Tool for the Classification of Raw Tobaccos

Abstract

The first part of the paper describes a new method of obtaining reproducible and meaningful headspace profiles of tobacco lamina by using a modified closed loop stripping apparatus. The complex chromatograms are obtained by high-resolution glass capillary gas chromatography. The second part summarizes the results of a chemometric approach to interpret the chromatograms obtained from a series of nine Virginia flue-cured tobaccos from different origins and belonging to different quality groups, each one analysed three times by the method described above. After the elimination of peaks containing redundant information, the resulting data set, consisting of 27 × 17 data points, was analysed to detect natural groupings by using an in-house program (in Basic) for principal component analysis. A subsequent discriminant analysis yielded two discriminant functions capable of separating the nine Virginia tobaccos into three quality groups as defined by a conventional organoleptic analysis carried out by a smoking panel. All the tobaccos could be classified correctly (100 %). A first attempt to classify, by the procedure described above, a group of six Virginia tobaccos whose organoleptic scores were not known, did not yield clearly interpretable results, possibly because the performance of the capillary column used for analysis had slightly deteriorated during the experiment with resultant changes in retention characteristics, which led to wrong identifications of certain peaks.

Qualitative and Quantitative Analysis of Flavor Additives on Tobacco Products Using SPME−GC−Mass Spectroscopy

“…Temperature Effects. Several headspace techniques have been reported for the analysis of tobacco (Coleman, 1992;Heinzer et al, 1988;Dirinck et al, 1980). Increase in sample temperature has been used in these traditional techniques to release volatiles into the headspace.…”

Tabakzusatzstoffe in Zigaretten – Verwendungszweck und Gefahrenpotential

“…In der Literatur Þ ndet man u. a. Methoden zur Bestimmung der Alkaloide in Tabak und im Rauch (Burns, 1977;Bush, 1972;Da Graca et al, 2000;Djordjevic et al, 1991;Jibao et al, 2003;Jones et al, 2001;Maru, 1998;Weeks et al, 1969;Wu et al, 2002;, zum Nachweis von ß üchtigen Verbindungen (Peng et al, 2004;Yang et al, 2002 II), zur Charakterisierung der natürlichen Hauptkomponenten, zur Bestimmung von Fettsäuren (Heinzer et al, 1988) sowie organischen Säuren (Clark und Bunch, 1996), Polyphenolen (Li et al, 2003), Alkenylbenzolen und anderen Aromastoffen (Clark und Bunch, 1997;StanÞ ll und Ashley, 1999;StanÞ ll et al, 2003) und Acetaten (Watson und Ashley, 2000).…”