Patterned Polyvinyl Alcohol Hydrogel Dressings with Stem Cells Seeded for Wound Healing

Abstract

Polyvinyl alcohol (PVA) hydrogel and stem cell therapy have been widely used in wound healing. However, the lack of bioactivity for PVA and security of stem therapy limited their application. In this study, an adipose-derived stem cells (ADSCs)-seeded PVA dressing (ADSCs/PVA) was prepared for wound healing. One side of the PVA dressing was modified with photo-reactive gelatin (Az-Gel) via ultraviolet (UV) irradiation (Az-Gel@PVA), and thus ADSCs could adhere, proliferate on the PVA dressings and keep the other side of the dressings without adhering to the wound. The structure and mechanics of Az-Gel@PVA were determined by scanning electron microscopy (SEM) and material testing instruments. Then, the adhesion and proliferation of ADSCs were observed via cell counts and live-dead staining. Finally, in vitro and in vivo experiments were utilized to confirm the effect of ADSCs/PVA dressing for wound healing. The results showed that Az-Gel was immobilized on the PVA and showed little effect on the mechanical properties of PVA hydrogels. The surface-modified PVA could facilitate ADSCs adhesion and proliferation. Protein released tests indicated that the bioactive factors secreted from ADSCs could penetrated to the wound. Finally, in vitro and in vivo experiments both suggested the ADSCs/PVA could promote the wound healing via secreting bioactive factors from ADSCs. It was speculated that the ADSCs/PVA dressing could not only promote the wound healing, but also provide a new way for the safe application of stem cells, which would be of great potential for skin tissue engineering.

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

“…Therefore, active components are usually introduced into PVA hydrogels through a physical or chemical process to facilitate stem cell growth and differentiation. Such active components include hyaluronic acid [13], chitosan [16], gelatin [17], oligopeptides [18], and karaya gum [19]. For example, when preparing PVA/chitosan composite hydrogels, increasing the proportion of PVA from 80 wt% to 90 wt% changes the stem cell shape from polygonal to spherical [16].…”

Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)‐based hydrogel substrates

“…PVA is a biocompatible and nontoxic polymer that has innumerous applications as a biomaterial (Gao et al, ; Gupta et al, ; Khorasani, Joorabloo, Adeli, Mansoori‐Moghadam, & Moghaddam, ; Liu et al, ; Teodorescu et al, ). Due to its excellent film‐forming properties and hydrophilic feature, PVA has been used as component of tissue engineering scaffolds in order to provide suitable strength and adhesive properties to support cell growth (Gupta et al, ; Liu et al, ; Rodríguez‐Rodríguez, García‐Carvajal, Jiménez‐Palomar, Jiménez‐Avalos, & Espinosa‐Andrews, ; Teodorescu et al, ).…”

“…PVA is a biocompatible and nontoxic polymer that has innumerous applications as a biomaterial (Gao et al, 2019;Gupta et al, 2011;Khorasani, Joorabloo, Adeli, Mansoori-Moghadam, & Moghaddam, 2019;Liu et al, 2009;Teodorescu et al, 2019). Due to its excellent film-forming properties and hydrophilic feature, PVA has been used as the free OH groups of the polymer, giving rise to ester linkages (Harifi & Montazer, 2012).…”

Visible-Light Active Titanium Dioxide Nanomaterials with Bactericidal Properties

“…The polymers employed for forming nanocomposites can be either degradable or nondegradable, depending on their intended application. Degradable polymers such as PLA, polyvinyl alcohol (PVA), poly(ε-caprolactone) (PCL) and chitosan are generally used to form scaffolds and wound dressings [26,65,[282][283][284][285]. These polymers should have the ability to degrade with time and to heal the wounds.…”