Patterning at the micro/nano-scale: Polymeric scaffolds for medical diagnostic and cell-surface interaction applications

Handrea-Dragan, Iuliana M.; Botiz, Ioan; Tatar, Andra-Sorina; Boca, Sanda

doi:10.1016/j.colsurfb.2022.112730

Cited by 15 publications

(4 citation statements)

References 285 publications

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“…It is worth noting that cells usually encounter fibrous, continuous structures when migrating in vivo , so exploring cell migration on continuous fibrous structures makes more sense [ 20 ]. To manufacture such microscale and nanoscale continuous structures, traditional methods, such as photolithography and molding are complex, expensive, and challenging to change dimensionally [ 21 , 22 ]. Electrowriting, which precisely controls fiber deposition through electrostatic force, can fabricate fibers within the desired size range while avoiding the previously mentioned drawbacks [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Electrowriting patterns and electric field harness directional cell migration for skin wound healing

Lai,

Huo,

Han

et al. 2024

Materials Today Bio

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Section: Introductionmentioning

confidence: 99%

Electrowriting patterns and electric field harness directional cell migration for skin wound healing

Lai,

Huo,

Han

et al. 2024

Materials Today Bio

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“…Advances in nanoscience continuously propel such a drive to create flexible and miniaturized biosensors, permitting high-throughput detection of bioanalytes that are held on an array of nanometer-sized sensor surfaces in a flexible setting. The majority of conventional biosensors are fabricated by top-down approaches such as photolithography, soft (microcontact printing) lithography, and inkjet printing, which can be costly and time-consuming [2,13,[17][18][19][20][21][22][23][24][25]. Fabrication techniques relying on conventional lithographic procedures also present limitations in the size of the smallest possible sensor unit that can be individually addressed.…”

Section: Introductionmentioning

confidence: 99%

“…Although there exist lithographic tools of higher spatial resolution such as electron-beam lithography [26][27][28], scanning probebased lithography [29,30] and nanoimprint lithography [31,32], the involvement of these procedures can lead to an even slower fabrication process and a higher production cost. Hence, alternative approaches based on self-assembly have emerged to create nanoscopic patterns of individually addressable biosensor surfaces and nanoscale bioreactors in simple steps [17,[33][34][35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Block Copolymers as a Facile Pathway to Create Functional Nanobiosensor and Nanobiomaterial Surfaces

Sytu,

Cho,

Hahm

2024

Polymers

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Block copolymer (BCP) surfaces permit an exquisite level of nanoscale control in biomolecular assemblies solely based on self-assembly. Owing to this, BCP-based biomolecular assembly represents a much-needed, new paradigm for creating nanobiosensors and nanobiomaterials without the need for costly and time-consuming fabrication steps. Research endeavors in the BCP nanobiotechnology field have led to stimulating results that can promote our current understanding of biomolecular interactions at a solid interface to the never-explored size regimes comparable to individual biomolecules. Encouraging research outcomes have also been reported for the stability and activity of biomolecules bound on BCP thin film surfaces. A wide range of single and multicomponent biomolecules and BCP systems has been assessed to substantiate the potential utility in practical applications as next-generation nanobiosensors, nanobiodevices, and biomaterials. To this end, this Review highlights pioneering research efforts made in the BCP nanobiotechnology area. The discussions will be focused on those works particularly pertaining to nanoscale surface assembly of functional biomolecules, biomolecular interaction properties unique to nanoscale polymer interfaces, functionality of nanoscale surface-bound biomolecules, and specific examples in biosensing. Systems involving the incorporation of biomolecules as one of the blocks in BCPs, i.e., DNA–BCP hybrids, protein–BCP conjugates, and isolated BCP micelles of bioligand carriers used in drug delivery, are outside of the scope of this Review. Looking ahead, there awaits plenty of exciting research opportunities to advance the research field of BCP nanobiotechnology by capitalizing on the fundamental groundwork laid so far for the biomolecular interactions on BCP surfaces. In order to better guide the path forward, key fundamental questions yet to be addressed by the field are identified. In addition, future research directions of BCP nanobiotechnology are contemplated in the concluding section of this Review.

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“…Microcontact printing techniques (μCPs) using PDMS stamps have been applied in many research fields, such as biopatterning, − (opto)electronics, ,− sensors, − materials science, , microfluidics, and others. Low cost and feasibility of the technique have been the reasons for the vast applications of different variations of μCP for decades.…”

Section: Introductionmentioning

confidence: 99%

Plasma Treatment of PDMS for Microcontact Printing (μCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells

Zemła,

Szydlak,

Gajos

et al. 2023

ACS Appl. Mater. Interfaces

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The present study investigates silicone transfer occurring during microcontact printing (μCP) of lectins with polydimethylsiloxane (PDMS) stamps and its impact on the adhesion of cells. Static adhesion assays and single-cell force spectroscopy (SCFS) are used to compare adhesion of nonmalignant (HCV29) and cancer (HT1376) bladder cells, respectively, to high-affinity lectin layers (PHA-L and WGA, respectively) prepared by physical adsorption and μCP. The chemical composition of the μCP lectin patterns was monitored by time-of-flight secondary ion mass spectrometry (ToF-SIMS). We show that the amount of transferred silicone in the μCP process depends on the preprocessing of the PDMS stamps. It is revealed that silicone contamination within the patterned lectin layers inhibits the adhesion of bladder cells, and the work of adhesion is lower for μCP lectins than for drop-cast lectins. The binding capacity of microcontact printed lectins was larger when the PDMS stamps were treated with UV ozone plasma as compared to sonication in ethanol and deionized water. ToF-SIMS data show that ozone-based treatment of PDMS stamps used for μCP of lectin reduces the silicone contamination in the imprinting protocol regardless of stamp geometry (flat vs microstructured). The role of other possible contributors, such as the lectin conformation and organization of lectin layers, is also discussed.

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Patterning at the micro/nano-scale: Polymeric scaffolds for medical diagnostic and cell-surface interaction applications

Cited by 15 publications

References 285 publications

Electrowriting patterns and electric field harness directional cell migration for skin wound healing

Electrowriting patterns and electric field harness directional cell migration for skin wound healing

Self-Assembled Block Copolymers as a Facile Pathway to Create Functional Nanobiosensor and Nanobiomaterial Surfaces

Plasma Treatment of PDMS for Microcontact Printing (μCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells

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