Patterning of cancerous cells driven by a combined modification of mechanical and chemical properties of the substrate

Raczkowska, Joanna; Awsiuk, Kamil; Prauzner-Bechcicki, Szymon; Pabijan, Joanna; Zemła, Joanna; Budkowski, Andrzej; Lekka, Małgorzata

doi:10.1016/j.eurpolymj.2017.01.006

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…Chloroform was used as a solvent for a PS film atop a PDMS matrix in order to create patterns that directed the positioning of bladder cancer cells (HV29s), whereby they selectively grew on the softer PDMS regions. 86 A commonly used soft lithography technique is replica molding (REM). It transfers topographical information from a mold to another material via a process of casting a prepolymer onto a predefined mold and then curing and peeling off the mold to leave behind a copy of the relief features from the master pattern.…”

Section: 29mentioning

confidence: 99%

“…The mold is inert to the solvent material, while the substrate dissolves or swells a thin layer of the substrate material, with the fluid or gel-like material created conforming to the mold’s relief structures. Chloroform was used as a solvent for a PS film atop a PDMS matrix in order to create patterns that directed the positioning of bladder cancer cells (HV29s), whereby they selectively grew on the softer PDMS regions …”

Section: Fabrication Of Micro- and Nanotopographic Surfaces For Cell ...mentioning

confidence: 99%

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The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Carthew

Taylor

Garcia-Cruz

et al. 2022

ACS Appl. Mater. Interfaces

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Cells sense and respond to a variety of physical cues from their surrounding microenvironment, and these are interpreted through mechanotransductive processes to inform their behavior. These mechanisms have particular relevance to stem cells, where control of stem cell proliferation, potency, and differentiation is key to their successful application in regenerative medicine. It is increasingly recognized that surface micro- and nanotopographies influence stem cell behavior and may represent a powerful tool with which to direct the morphology and fate of stem cells. Current progress toward this goal has been driven by combined advances in fabrication technologies and cell biology. Here, the capacity to generate precisely defined micro- and nanoscale topographies has facilitated the studies that provide knowledge of the mechanotransducive processes that govern the cellular response as well as knowledge of the specific features that can drive cells toward a defined differentiation outcome. However, the path forward is not fully defined, and the “bumpy road” that lays ahead must be crossed before the full potential of these approaches can be fully exploited. This review focuses on the challenges and opportunities in applying micro- and nanotopographies to dictate stem cell fate for regenerative medicine. Here, key techniques used to produce topographic features are reviewed, such as photolithography, block copolymer lithography, electron beam lithography, nanoimprint lithography, soft lithography, scanning probe lithography, colloidal lithography, electrospinning, and surface roughening, alongside their advantages and disadvantages. The biological impacts of surface topographies are then discussed, including the current understanding of the mechanotransductive mechanisms by which these cues are interpreted by the cells, as well as the specific effects of surface topographies on cell differentiation and fate. Finally, considerations in translating these technologies and their future prospects are evaluated.

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Section: 29mentioning

confidence: 99%

Section: Fabrication Of Micro- and Nanotopographic Surfaces For Cell ...mentioning

confidence: 99%

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Carthew

Taylor

Garcia-Cruz

et al. 2022

ACS Appl. Mater. Interfaces

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“…Similar tendencies for differences in fibrinogen adsorption on spin-coated poly(isobutyl methacrylate), poly(butyl methacrylate), and poly(lauryl methacrylate) with different flexibilities at room temperature have also been described . In some works, − the strong impact of substrate elasticity on cell behavior has been shown. Moreover, in the work by Raczkowska et al, the highly cell-dependent impact of the mechanical properties on cellular behavior has been shown for cells co-cultured from the mixture.…”

Section: Introductionmentioning

confidence: 60%

Temperature-Controlled Orientation of Proteins on Temperature-Responsive Grafted Polymer Brushes: Poly(butyl methacrylate) vs Poly(butyl acrylate): Morphology, Wetting, and Protein Adsorption

et al. 2019

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Poly(n-butyl methacrylate) (PBMA) or poly(n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glasstransition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around −55 °C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG.

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“…Among these processes, the proliferation of fibroblasts plays an important role in the production of extracellular matrix (ECM) proteins. Contrary to what happens in pathological conditions or tissue damage, these fibroblasts sustain the interstitial medium of the cardiomyocytes and regulate the supporting structure of the heart, actively participating in the healing process [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Development of endomyocardial fibrosis model using a cell patterning technique: In vitro interaction of cell coculture of 3T3 fibroblasts and RL-14 cardiomyocytes

2020

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Cardiac functions can be altered by changes in the microstructure of the heart, i.e., remodeling of the cardiac tissue, which may activate pathologies such as hypertrophy, dilation, or cardiac fibrosis. Cardiac fibrosis can develop due to an excessive deposition of extracellular matrix proteins, which are products of the activation of fibroblasts. In this context, the anatomical-histological change may interfere with the functioning of the cardiac tissue, which requires specialized cells for its operation. The purpose of the present study was to determine the cellular interactions and morphological changes in cocultures of 3T3 fibroblasts and RL-14 cardiomyocytes via the generation of a platform an in vitro model. For this purpose, a platform emulating the biological characteristics of endomyocardial fibrosis was generated using a cell patterning technique to study morphological cellular changes in compact and irregular patterns of fibrosis. It was found that cellular patterns emulating the geometrical distributions of endomyocardial fibrosis generated morphological changes after interaction of the RL-14 cardiomyocytes with the 3T3 fibroblasts. Through this study, it was possible to evaluate biological characteristics such as cell proliferation, adhesion, and spatial distribution, which are directly related to the type of emulated endomyocardial fibrosis. This research concluded that fibroblasts inhibited the proliferation of cardiomyocytes via their interaction with specific microarchitectures. This behavior is consistent with the histopathological distribution of cardiac fibrosis; therefore, the platform developed in this research could be useful for the in vitro assessment of cellular microdomains. This would allow for the experimental determination of interactions with drugs, substrates, or biomaterials within the engineering of cardiac tissues.

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Patterning of cancerous cells driven by a combined modification of mechanical and chemical properties of the substrate

Cited by 5 publications

References 45 publications

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Temperature-Controlled Orientation of Proteins on Temperature-Responsive Grafted Polymer Brushes: Poly(butyl methacrylate) vs Poly(butyl acrylate): Morphology, Wetting, and Protein Adsorption

Development of endomyocardial fibrosis model using a cell patterning technique: In vitro interaction of cell coculture of 3T3 fibroblasts and RL-14 cardiomyocytes

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