Patterning of Protein/Quantum Dot Hybrid Bionanostructures

Nandwana, Vikas; Mout, Rubul; Yeh, Yi Cheun; Dickert, Stefan; Tuominen, Mark; Rotello, Vincent M.

doi:10.1007/s10904-012-9772-y

Cited by 9 publications

(17 citation statements)

References 40 publications

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“…The explanation presented by Werts et al has been corroborated by later works (94,97,98,113,115,116,(121)(122)(123) to the extent that they rule out the initial hypothesis of ligand stripping. Recently, (113,115) we have shown by ex situ and in situ X-ray photoelectron spectroscopy (XPS) and X-ray-excited Auger electron spectroscopy (XAES) that carbon hybridization of capping ligands on an NC film goes from sp 3 to sp 2 (see Figure 1b-d), meaning that C-H bonds are cleaved and C═C bonds are formed.…”

Section: Fundamentals Of Direct-write Lithography On Nc Filmsmentioning

confidence: 76%

Writing on Nanocrystals: Patterning Colloidal Inorganic Nanocrystal Films through Irradiation-Induced Chemical Transformations of Surface Ligands

2017

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In the past couple of decades, colloidal inorganic nanocrystals (NCs) and, more specifically, semiconductor quantum dots (QDs) have emerged as crucial materials for the development of nanoscience and nanotechnology, with applications in very diverse areas such as optoelectronics and biotechnology. Films made of inorganic NCs deposited on a substrate can be patterned by e-beam lithography, altering the structure of their capping ligands and thus allowing exposed areas to remain on the substrate while non-exposed areas are redispersed in a solvent, as in a standard lift-off process. This methodology can be described as a “direct” lithography process, since the exposure is performed directly on the material of interest, in contrast with conventional lithography which uses a polymeric resist as a mask for subsequent material deposition (or etching). A few reports from the late 1990s and early 2000s used such direct lithography to fabricate electrical wires from metallic NCs. However, the poor conductivity obtained through this process hindered the widespread use of the technique. In the early 2010s, the same method was used to define fluorescent patterns on QD films, allowing for further applications in biosensing. For the past 2–3 years, direct lithography on NC films with e-beams and X-rays has gone through an important development as it has been demonstrated that it can tune further transformations on the NCs, leading to more complex patternings and opening a whole new set of possible applications. This Perspective summarizes the findings of the past 20 years on direct lithography on NC films with a focus on the latest developments on QDs from 2014 and provides different potential future outcomes of this promising technique.

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Section: Fundamentals Of Direct-write Lithography On Nc Filmsmentioning

confidence: 76%

Writing on Nanocrystals: Patterning Colloidal Inorganic Nanocrystal Films through Irradiation-Induced Chemical Transformations of Surface Ligands

2017

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“…We first fabricated two AuNPs (∼2 nm core), one featuring a cationic hydrophobic (NP1) and the other a hydrophilic (NP2) functional group ( Figure 2 A) These particles were screened from a library of different particles including aromatic and aliphatic headgroups, first to determine the individual particles and then to optimize their ratio in the sensor. Multichannel output is provided through reversible adsorption followed by partial displacement of three fluorescent proteins with well-separated excitation and emission spectra (Figure S1): red (tdTomato), 28 blue (EBFP2), 29 and green (EGFP). 30 These proteins feature negative surface charge, allowing electrostatic interactions with cationic NP1 and NP2.…”

Section: Resultsmentioning

confidence: 99%

Rapid Identification of Bacterial Biofilms and Biofilm Wound Models Using a Multichannel Nanosensor

Li¹,

Kong

Mout³

et al. 2014

ACS Nano

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Identification of infectious bacteria responsible for biofilm-associated infections is challenging due to the complex and heterogeneous biofilm matrix. To address this issue and minimize the impact of heterogeneity on biofilm identification, we developed a gold nanoparticle (AuNP)-based multichannel sensor to detect and identify biofilms based on their physicochemical properties. Our results showed that the sensor can discriminate six bacterial biofilms including two composed of uropathogenic bacteria. The capability of the sensor was further demonstrated through discrimination of biofilms in a mixed bacteria/mammalian cell in vitro wound model.

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“…There are no covalent linkages between QDs and proteins. The structural stability of the protein–QD hybrids relies on multiple noncovalent interactions, such as electrostatic assembly, hydrophobic bonding and metal‐affinity coordination, as well as the conformational stability of the hybridized proteins . To increase the stability of the protein–QD hybrids, multivalent protein ligands (e.g., multimeric proteins or dendrimeric peptides) have been introduced, significantly increasing the affinity of proteins to QDs in biological fluids …”

Section: Surface Modification Strategymentioning

confidence: 99%

“…The structural stability of the protein-QD hybrids relies on multiple noncovalent interactions, such as electrostatic assembly, hydrophobic bonding and metal-affinity coordination, as well as the conformational stability of the hybridized proteins. 28,29 To increase the stability of the protein-QD hybrids, multivalent protein ligands (e.g., multimeric proteins or dendrimeric peptides) have been introduced, significantly increasing the affinity of proteins to QDs in biological fluids. 30,31 Recently, we also proposed new tumor-targeted protein-QD hybrids based on shell cross-linked nanocapsules, which are composed of human serum albumin cross-linked with multiarmed polyethylene glycol (PEG).…”

Section: Self-assembly Of Qds and Proteinsmentioning

confidence: 99%

Protein–quantum dot nanohybrids for bioanalytical applications

Lee

Kim

Park

et al. 2015

WIREs Nanomed Nanobiotechnol

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Quantum dots (QDs) coupled with biomolecules play an important role as optically and chemically stable bioimaging agents for various applications. These inorganic-biological hybrid conjugates have been demonstrated as powerful fluorescence tools for sensing, diagnostics, and labeling. This review focuses on protein-QD nanohybrids for different types of bioanalytical applications. There are various strategies to modify the surface properties of QDs to produce protein-QD nanohybrids that are stable in biological fluids. We expect that multifunctional protein-QD nanohybrids can be used as a powerful optical probe for various biological applications. WIREs Nanomed Nanobiotechnol 2016, 8:178-190. doi: 10.1002/wnan.1345 For further resources related to this article, please visit the WIREs website.

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Patterning of Protein/Quantum Dot Hybrid Bionanostructures

Cited by 9 publications

References 40 publications

Writing on Nanocrystals: Patterning Colloidal Inorganic Nanocrystal Films through Irradiation-Induced Chemical Transformations of Surface Ligands

Writing on Nanocrystals: Patterning Colloidal Inorganic Nanocrystal Films through Irradiation-Induced Chemical Transformations of Surface Ligands

Rapid Identification of Bacterial Biofilms and Biofilm Wound Models Using a Multichannel Nanosensor

Protein–quantum dot nanohybrids for bioanalytical applications

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