Patterning of Quantum Dot Bioconjugates via Particle Lithography

Abstract

We present a simple technique to fabricate hexagonally ordered quantum dot bioconjugate (QDBC) dot arrays on glass coverslips. We used particle lithography to create periodic holes in a layer of methoxy-poly(ethylene glycol)-silane and then adsorbed QDBCs into the holes. To demonstrate the versatility of this technique, we made separate periodic arrays of quantum dots (QDs) conjugated to three different biologically important molecules: biotin, streptavidin, and anti-mouse IgG. The diameters of the regions where the QDBCs adsorbed were 500-600 nm and independent of the QDBC patterned. The site density of the QDBCs in the patterned holes could be varied by simply adjusting the coating concentration of the QDBC solution. We demonstrate the applicability of these substrates by designing a QDBC-based binding assay with a working concentration range of several orders of magnitude and a sub-picomolar detection limit.

Synthesis of carbon‐based quantum dots from starch extracts: Optical investigations

“…It has also been shown that such assemblies exhibited efficient quantum dot − quantum dot and quantum dot − dye energy transfer, which accommodated faster energy‐transfer rates as compared with densely packed quantum dot arrays placed on planar substrates. Taylor et al presented a simple approach to fabricate hexagonally ordered quantum dot bioconjugate (QDBC) arrays on glass coverslips. A particle lithography was used to create periodic holes on a layer of methoxy‐poly(ethylene glycol)‐silane and then have QDBCs adsorbed into the holes.…”

Identification of Dewetting Stages and Preparation of Single Chain Gold Nanoparticle Rings by Colloidal Lithography

“…Positive or negative surface corrugations at the length scale of the assembled nanoparticles have been shown to effectively direct the particle positioning, , whereas self-assembled microparticle monolayers offer a convenient bottom-up template type for the massively parallel assembly of nanoscale objects. The concept of using larger microspheres was successfully applied earlier to create circular or localized patterns form quantum dots, quantum dot bioconjugates, or even gold nanoparticles. , These examples together with others, where the circular structures are formed directly from precursors in the liquid that was trapped beneath the microspheres, show that dewetting in a confined geometry can enable the preparation of circular structures from different materials. Unfortunately, due to the large number of parameters that determine the structure (e.g., template size, surface energies, speed of evaporation), unambiguous identification of the key parameters is rather difficult.…”

Independently Controlling Protein Dot Size and Spacing in Particle Lithography

“…Protein patterns with micro- and nanometer scale features have been fabricated by a number of different techniques, including microfluidic patterning, − photolithography, − electron-beam lithography (EBL), , microcontact printing (μCP), − dip-pen nanolithography (DPN), , and particle lithography. − There are several factors to consider when choosing a technique for a specific application, including desired feature size and geometry, cost, processing time, and accessibility. Particle lithography is an attractive option to pattern protein because it relies on low cost, benchtop technology with the ability to produce nanostructured samples in parallel.…”