Patterning parameters for biomolecules microarrays constructed with nanosecond and femtosecond UV lasers

Dinca, Valentina; Farsari, Maria; Kafetzopoulos, Dimitris; Popescu, A.; Dinescu, M.; Fotakis, C.

doi:10.1016/j.tsf.2008.02.043

Cited by 77 publications

(48 citation statements)

References 36 publications

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“…The technique has also a high degree of spatial resolution: under appropriate irradiation conditions the material can be deposited in the form of single microdroplets, 9 which can have diameters as small as a few microns. 10 Moreover, LIFT can be used for printing different complex materials, such as inorganic inks or pastes, 11 organic polymers, 12 and even biological solutions. 8,[13][14][15] The feasibility of the technique for depositing these materials has been proved through the fabrication of diverse functional devices such as microbatteries, 11 solar cells, 16 organic light-emitting diodes, 17 or biosensors.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 Such interesting features prompted several studies on the transfer process which takes place during the LIFT of liquids. These studies 9,10,14,15,20 were mainly focused on the analysis of the effects that different technological parameters have on the morphology of the transferred material. This allowed improving the performance of the technique, although it also revealed the need for the elucidation of the transfer mechanisms in order to set the basis for parameters optimization.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time-resolved imaging of the laser forward transfer of liquids

Duocastella¹,

Fernández-Pradas²,

Morenza³

et al. 2009

Journal of Applied Physics

138

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Time-resolved imaging is carried out to study the dynamics of the laser-induced forward transfer of an aqueous solution at different laser fluences. The transfer mechanisms are elucidated, and directly correlated with the material deposited at the analyzed irradiation conditions. It is found that there exists a fluence range in which regular and well-defined droplets are deposited. In this case, laser pulse energy absorption results in the formation of a plasma, which expansion originates a cavitation bubble in the liquid. After the further expansion and collapse of the bubble, a long and uniform jet is developed, which advances at a constant velocity until it reaches the receptor substrate. On the other hand, for lower fluences no material is deposited. In this case, although a jet can be also generated, it recoils before reaching the substrate. For higher fluences, splashing is observed on the receptor substrate due to the bursting of the cavitation bubble. Finally, a discussion of the possible mechanisms which lead to such singular dynamics is also provided.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Time-resolved imaging of the laser forward transfer of liquids

Duocastella¹,

Fernández-Pradas²,

Morenza³

et al. 2009

Journal of Applied Physics

138

View full text Add to dashboard Cite

show abstract

“…In this approach, the material of interest is dissolved or suspended in a liquid, and submitted to LIFT, leading to the deposition of the solution/suspension in the form of microdroplets. A great variety of complex materials has been successfully printed through LIFT: nanoparticle inks for electrodes in transistors [7,8], polymers for chemical sensors [9], biomolecule solutions for biosensors [10][11][12][13], or even living cells for tissue engineering applications [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Film-free laser forward printing of transparent and weakly absorbing liquids

et al. 2010

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A laser-based technique for printing transparent and weakly absorbing liquids is developed. Its principle of operation relies in the tight focusing of short laser pulses inside the liquid and close to its free surface, in such a way that the laser radiation is absorbed in a tiny volume around the beam waist, with practically no absorption in any other location along the beam path. If the absorbed energy overcomes the optical breakdown threshold, a cavitation bubble is generated, and its expansion results in the propulsion of a small fraction of liquid which can be collected on a substrate, leading to the printing of a microdroplet for each laser pulse. The technique does not require the preparation of the liquid in thin film form, and its forward mode of operation imposes no restriction concerning the optical properties of the substrate. These characteristics make it well suited for printing a wide variety of materials of interest in diverse applications. We demonstrate that the film-free laser forward printing technique is capable of printing microdroplets with good resolution, reproducibility and control, and analyze the influence of the main process parameter, laser pulse energy. The mechanisms of liquid printing are also investigated: timeresolved imaging provides a clear picture of the dynamics of liquid transfer which allows understanding the main features observed in the printed droplets.

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“…They are combined with different DRL materials, including metals (gold, silver, or titanium), polymers (triazene, polyethylene naphthalate, polyimide, or cyanoacrylate), or hydrogels (gelatin). Most groups using LaBP for printing biomaterials apply ultraviolet (UV) lasers with 3 to 30 nanoseconds pulse durations and 193-nm [1,2] , 248-nm [3,4] , 266-nm [5] , 337-nm [6] , or 355-nm [7,8] wavelengths.…”

Section: Introductionmentioning

confidence: 99%

“…In spite of this wide range of applied laser parameters, so far, their impact on the transfer process has hardly been analyzed in direct comparison-with the exception of laser pulse energy, laser pulse intensity, and the focal spot size. There is one publication, in which Dinca et al [4] utilized laser-printed proteins and DNA with 500 femtoseconds of pulse duration at 248-nm wavelength and compared the results with those achieved with 15-ns pulse duration.…”

Section: Introductionmentioning

confidence: 99%

Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study

Koch

Brandt

Deiwick

et al. 2017

ijb

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For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bioprinting. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial. Different lasers with laser wavelengths from 193 to 1064 nanometer have been used. As a metal DRL gold, silver, or titanium layers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these lasers parameters on bioprinting with a metal DRL, and on comparing different wavelengths and pulse durations -except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration (in the range of 8 to 200 ns) are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell viability are analyzed. Keywords: bioprinting, laser-assisted bioprinting, laser-induced forward transfer, laser absorption layer, laser parametric study *Correspondence to: Lothar Koch, Laser Zentrum Hannover e.V., Nanotechnology Department, Hollerithallee 8, 30419 Hannover, Germany; Email: l.koch@lzh.de

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Patterning parameters for biomolecules microarrays constructed with nanosecond and femtosecond UV lasers

Cited by 77 publications

References 36 publications

Time-resolved imaging of the laser forward transfer of liquids

Time-resolved imaging of the laser forward transfer of liquids

Film-free laser forward printing of transparent and weakly absorbing liquids

Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study

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