Patterns of gene expression and the cellular origins of human leukaemias

Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies

“…These observations confirm that most NHL are of B-cell origin and also show that NHL in the younger age group (which relatively frequently derive from non-B-cell types; Bernard et al, 1979;Habeshaw, 1980) can be characterized in tissue sections with the reagents already available for diagnosis of leukaemia. These include antisera to TdT, Ia-like, HuTLA and HTA-1 antigens (Table I; Bollum, 1975; reviewed by Greaves & Janossy, 1978;Janossy, 1980). In particular, the antibody to TdT detects TdT+ blast cells in FF paraffin-embedded sections (Fig.…”

“…First, interchangeable filter sets used on the modern microscopes facilitate the simultaneous application of antibodies labelled with fluorescein-isothiocyanate (FITC-green) and tetraethylrhodamine-isothiocyanate (TRITC-red). Second, specific antisera and purified antibodies (eluted from immunoabsorbent columns) have been introduced for the immunodiagnosis of leukaemia (Thierfelder et al, 1977;Bollum, 1975;Greaves & Janossy, 1978) and can also be applied for lymphoma diagnosis. Third, the production of "monoclonal" antibodies by somatic-cell hybridization (Kohler & Milstein, 1975) has now been established as an ideal method for preparing standard reagents which show low or undetectable nonspecific background staining in tissue sections Janossy et al, 198ob).…”

Clinical flow cytometry, a hypothesis‐driven discipline of modern cytomics

“…2-13) (14,17). When the anti-non-T, non-B ALL antiserum was cross-absorbed with PhЈ lymphoid blasts ( Fig.…”

“…Hypothesis 1A: A non-T, non-B ALL antiserum will react with (a small number of) normal precursor cells, the "target cells," from which this particular form of malignancy derives (14).…”

“…As predicted, this was the first antiprecursor cell reagent; the reactivity is shown in Table 2. Once this cell type, also referred to as the pro-B cell, and its close relative, the cytoplasmic immunoglobulin (Ig) positive pre-B cells were identified, these cell types were characterized extensively by flow cytometry, cell sorting, biochemistry, and gene rearrangements (2,14) (also see below).…”