Patterns of Immune Infiltration and the Key Immune-Related Genes in Acute Type A Aortic Dissection in Bioinformatics Analyses

Chen, Fengshou; Han, Jie; Tang, Bing

doi:10.2147/ijgm.s317405

Cited by 17 publications

(7 citation statements)

References 55 publications

(56 reference statements)

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“…Interestingly, our study found low abundance of γδT cells in aortic dissection vessel tissue. This is in accordance with several other studies which also found low levels of γδT cells, although some were not statistically significant [ 20 – 23 ]. We think the cause may be as follows.…”

Section: Discussionsupporting

confidence: 93%

Identification of key biomarkers and immune infiltration in the thoracic acute aortic dissection by bioinformatics analysis

Luo

Shi

Ran

et al. 2023

BMC Cardiovasc Disord

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Background Thoracic acute aortic dissection (TAAD), one of the most fatal cardiovascular diseases, leads to sudden death, however, its mechanism remains unclear. Methods Three Gene Expression Omnibus datasets were employed to detect differentially expressed genes (DEGs). A similar function and co-expression network was identified by weighted gene co-expression network analysis. The least absolute shrinkage and selection operator, random forest, and support vector machines-recursive feature elimination were utilized to filter diagnostic TAAD markers, and then screened markers were validated by quantitative real-time PCR and another independent dataset. CIBERSORT was deployed to analyze and evaluate immune cell infiltration in TAAD tissues. Results Twenty-five DEGs were identified and narrowed down to three after screening. Finally, two genes, SLC11A1 and FGL2, were verified by another dataset and qRT-PCR. Function analysis revealed that SLC11A1 and FGL2 play significant roles in immune-inflammatory responses. Conclusion SLC11A1 and FGL2 are differently expressed in aortic dissection and may be involved in immune-inflammatory responses.

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Section: Discussionsupporting

confidence: 93%

Identification of key biomarkers and immune infiltration in the thoracic acute aortic dissection by bioinformatics analysis

Luo

Shi

Ran

et al. 2023

BMC Cardiovasc Disord

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“…5,6 Despite the advances in surgical techniques and perioperative management, the in-hospital mortality rate of Stanford type A AD patients remains high. [7][8][9] Recently, some prognostic factors have been identified to predict the in-hospital survival of Stanford type A AD patients, 10,11 while discovering more potential AD biomarkers is still necessary and remains a critical issue for clinicians.…”

Section: Introductionmentioning

confidence: 99%

Th1, Th2, and Th17 cells are dysregulated, but only Th17 cells relate to C‐reactive protein, D‐dimer, and mortality risk in Stanford type A aortic dissection patients

Song

Deng

Shen

et al. 2022

Clinical Laboratory Analysis

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Background: T helper (Th) cells are closely involved in vascular inflammation, endothelial dysfunction, and atherogenesis, which are the hallmarks of aortic dissection (AD). This study aimed to evaluate the clinical value of Th1, Th2, and Th17 cell measurements in Stanford type A AD patients. Methods: Stanford type A AD patients (N=80) and non-AD patients with chest pain (N = 40) were recruited. Then, Th1, Th2, and Th17 cells in peripheral blood CD4 + T cells from all participants were detected by flow cytometry. The 30-day mortality of Stanford type A AD patients was recorded.Results: Th1 and Th17 cells were higher, while Th2 cells were lower in Stanford type A AD patients compared with non-AD patients (all p < 0.001). Meanwhile, Th1 cells (area under curve (AUC): 0.734, 95% confidence interval (CI): 0.640-0.828), Th2 cells (AUC: 0.841, 95% CI: 0.756-0.925), and Th17 cells (AUC: 0.898, 95% CI: 0.839-0.957) could distinguish Stanford type A patients from non-AD patients. Moreover, Th1 cells (p = 0.037) and Th17 cells (p = 0.001) were positively related to CRP, and Th17 cells (p = 0.039) were also positively associated with D-dimer in Stanford type A AD patients. Furthermore, Th17 cells were elevated in deaths compared with survivors (p = 0.001), also, it could estimate 30-day mortality risk in Stanford type A AD patients with an AUC of 0.741 (95% CI: 0.614-0.867), which was similar to the value of CRP (AUC: 0.771, 95% CI: 0.660-0.882), but lower than the value of D-dimer (AUC:

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“…However, a cluster of Th17-like T cells was found to expand in the AD group. Previous studies also reported elevation of Th17 cells both in AD tissues and blood samples of AD patients ( 50 , 51 ). Ju et al ( 52 ) observed Th17-cell accumulation in both patients and mouse models of aortic dissection.…”

Section: Discussionmentioning

confidence: 59%

Single-Cell Sequencing of Immune Cells in Human Aortic Dissection Tissue Provides Insights Into Immune Cell Heterogeneity

Liu

Zou

Tang

et al. 2022

Front. Cardiovasc. Med.

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BackgroundInflammation plays an important role in the progression of sporadic aortic dissection (AD). Immune cells, especially macrophages, infiltrate the aorta and secrete inflammatory cytokines and matrix metalloproteinases to cause degradation of the extracellular matrix, thereby contributing to the pathogenesis of AD. However, the cellular heterogeneity within these immune cells has not been fully characterized.MethodsWe used single-cell RNA sequencing to profile the transcriptomes of all immune cells in AD tissue and normal aorta. Using magnetic-activated cell sorting gating on CD45, we obtained a higher resolution identification of the immune cell subsets in the aorta.ResultsWe observed significant differences in the proportion of major immune cell subpopulations between AD and normal aorta tissues. Macrophages accounted for a higher percentage in the normal aorta, while the proportions of T cells, B cells and natural killer (NK) cells were all increased in AD tissues. Macrophage clusters that expanded in AD tissues originated primarily from circulating monocytes and expressed genes encoding proinflammatory cytokines and molecules involved in tissue repair. T and NK cells in AD tissues exhibited enhanced cytotoxic properties. A cluster of CD4+ T cells that had expanded in AD tissues was Th17-like and might contribute to the pathogenesis of AD. Cell–cell interaction analysis highlighted the increased communication between macrophages and T cells, which primarily regulated the costimulation of T cells.ConclusionsOur study provides a comprehensive characterization of immune cells in the dissected aorta with an emphasis on the role of macrophages and T cells. The information from our study improves our understanding of immune mechanisms in AD formation and helps to identify additional useful targets for early diagnosis or therapy of AD.

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Patterns of Immune Infiltration and the Key Immune-Related Genes in Acute Type A Aortic Dissection in Bioinformatics Analyses

Cited by 17 publications

References 55 publications

Identification of key biomarkers and immune infiltration in the thoracic acute aortic dissection by bioinformatics analysis

Identification of key biomarkers and immune infiltration in the thoracic acute aortic dissection by bioinformatics analysis

Th1, Th2, and Th17 cells are dysregulated, but only Th17 cells relate to C‐reactive protein, D‐dimer, and mortality risk in Stanford type A aortic dissection patients

Single-Cell Sequencing of Immune Cells in Human Aortic Dissection Tissue Provides Insights Into Immune Cell Heterogeneity

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