Patterns of mycotoxin production by Fusarium gmminearum isolated from Argentine wheat

Faifer, Graciela C.; Miguel, Mauricia Sala De; Godoy, Héctor M.

doi:10.1007/bf00436805

Cited by 29 publications

(11 citation statements)

References 16 publications

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“…None of the isolates produced both DON and NIV. The levels and the type of toxin found in the present work agree with previous studies carried out in our country (Faifer et al, 1990;Molto et al, 1997). Faifer et al, (1990) tested 24 F. graminearum strains isolated from wheat for mycotoxin production, and found that only 33% of the evaluated strains were able to produce DON, 15-ADON and 3-ADON.…”

Section: Discussionsupporting

confidence: 93%

Vegetative Compatibility and Mycotoxin Chemotypes among Fusarium graminearum (Gibberella zeae) Isolates from Wheat in Argentina

et al. 2006

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Sumíková T., Gabrielová L., Kučera L., Žabka M., Chrpová J. (2013): Mycotoxin production, chemo-types and diversity of Czech Fusarium graminearum isolates on wheat. Czech J. Food Sci., 31: 407-412. Fusarium head blight (FHB) is a serious cereal disease in the Czech Republic. The most important pathogen associated with FBH is Fusarium graminearum, which can produce trichothecenes, mainly deoxynivalenol (DON) and its derivates. A set of 103 F. graminearum isolates were isolated from naturally infected wheat ears collected from 20 localities (25 ears from one locality) within the Czech Republic, in the year 2004. The ears were evaluated for DON content by ELISA. Maximum detected value was 30.7 mg/kg. A group of PCR assays targeting the segments of the Tri7, Tri13 and Tri3 genes were used to determine the chemotypes of F. graminearum isolates. All the isolates belonged to DON producing chemotype. Further discrimination revealed that almost all (99.03%) isolates belonged to 15-ADON chemotype, and only one (0.97%) isolate belonged to 3-ADON chemotype. The genetic variability of the isolates was assessed from their AFLP fingerprints. The populations were highly heterogeneous both within and between locations, and no clear evidence for the association between AFLP profile and geographic origin was found out.

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Section: Discussionsupporting

confidence: 93%

Vegetative Compatibility and Mycotoxin Chemotypes among Fusarium graminearum (Gibberella zeae) Isolates from Wheat in Argentina

et al. 2006

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“…Nevertheless, it is also well established that, within the same species and under optimal conditions of growth, some strains produce large amounts of trichothecenes, whereas other strains produce small or undetectable amounts of trichothecenes (15,19,24,30,35,36,57). Our results are consistent with these observations.…”

Section: Discussionsupporting

confidence: 92%

Identification by PCR of Fusarium culmorum Strains Producing Large and Small Amounts of Deoxynivalenol

Bakan

Giraud-Delville

Pinson

et al. 2002

Appl Environ Microbiol

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Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum strains produced less than 0.07 ppm of trichothecenes and were considered low-deoxynivalenol-producing strains. For all strains, a 550-base portion of the trichodiene synthase gene (tri5) was amplified and sequenced. According to the tri5 data, the F. culmorum strains tested clustered into two groups that correlated with in vitro deoxynivalenol production. For three high-producing and three low-producing F. culmorum strains, the tri5-tri6 intergenic region was then sequenced, which confirmed the two separate clusters within the F. culmorum strains. According to the tri5-tri6 sequence data, specific PCR primers were designed to allow differentiation of high-producing from low-producing F. culmorum strains.Trichothecenes, including deoxynivalenol, acetyldeoxynivalenol, nivalenol, and fusarenone X, are sesquiterpene toxins produced by Fusarium species, including Fusarium culmorum, which are common fungal contaminants of cereals. Trichothecenes can be found naturally worldwide on cereals (1,9,18,27,38,45,48,54,55,59), and the consumption of these toxins is a potential problem for humans and farm animals (14, 47).It has been established that some Fusarium species, including F. graminearum and F culmorum, are able to produce B trichothecenes, such as deoxynivalenol and acetyldeoxynivalenol, while other species are not (31). These two types of Fusarium strains (producers and nonproducers) can be distinguished on the basis of DNA polymorphism in the ␤-tubulin gene (43) as well as in the large ribosomal subunit or the internal transcribed spacer (21,37,43).According to their trichothecene production, some Fusarium species, such as F graminearum, have been divided into two chemotypes: (i) the nivalenol chemotype, which includes isolates producing nivalenol and fusarenone X, and (ii) the deoxynivalenol chemotype, which includes isolates producing deoxynivalenol and acetyldeoxynivalenol (26,53). Similar observations have been made for F. culmorum strains (28, 39).In addition, it has been demonstrated that, within the same species and in the same culture conditions, toxin production by Fusarium strain may vary sharply; some strains produce large amounts of trichothecenes, whereas others produce small or undetectable amounts of trichothecenes (3,5,19,28,30,35,36,39,53,57). Until now, no method except in vitro culture has been available to distinguish high-producing from low-producing Fusarium strains.Several genes involved in the biosynthesis of trichothecenes have been described, most of them localized in a gene cluster. The tri5 gene encodes the trichodiene synthase, which catalyzes the first step in the biosynthesis of trichothecenes. The nucleotide sequence of the tri5 gene has been characterized in several Fusa...

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“…The ability of Argentinean Fusarium isolates to produce trichothecenes is controversial and the pattern of micotoxin production is not already well defined. Faifer et al (8) reported that F. graminearum isolated from wheat in Argentina produced DON, 15-acetyldeoxinivalenol (15AcDON), 3-acetyldeoxinivalenol (3AcDON) and zearalenone (ZEA) while Ramirez et al (20) found only DON and 3-AcDON producers. On the other hand, Lori (15) studied the capability of 76 F. graminearum strains isolated from Argentinean wheat and 56% produced DON, 3AcDON, 10% NIV, FUS-X and 34% both DON and NIV.…”

Section: Introductionmentioning

confidence: 99%

Natural occurrence of nivalenol and mycotoxigenic potential of Fusarium graminearum strains in wheat affected by head blight in Argentina

Pinto¹,

Terminiello²,

Basílico³

et al. 2008

Braz. J. Microbiol.

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The principal agents of Fusarium head blight in the main cropping area of Argentina were investigated in heavily infected samples. The ability of the isolates to produce trichothecenes was determined by GC and HPLC. Fusarium graminearum was the predominant species and of 33 isolates, 10 produced deoxinivalenol (DON) (0.1-29 mg kg ), 10 produced Fusarenone X (0.1-2.4 mg kg -1 ) and 7 produced zearalenone (0.1-0.6 mg kg -1 ). These results suggest that F. graminearum strains isolated from the wheat growing regions in Argentina belong to DON chemotype. Although some strains produced both deoxinivalenol and nivalenol, nivalenol was produced in lower levels. The natural occurrence of nivalenol in wheat affected by head-blight collected in the main production area during two years (2001)(2002) was also determined. From 19 samples 13 were contaminated with deoxinivalenol in a range of 0.3 to 70 mg kg -1 and 2 samples with both deoxinivalenol (7.5 and 6.7 mg kg -1 ) and nivalenol (0.05 and 0.1 mg kg -1 ), respectively. This is the first report of natural occurrence of nivalenol in wheat cultivate in Argentina.

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Patterns of mycotoxin production by Fusarium gmminearum isolated from Argentine wheat

Cited by 29 publications

References 16 publications

Vegetative Compatibility and Mycotoxin Chemotypes among Fusarium graminearum (Gibberella zeae) Isolates from Wheat in Argentina

Vegetative Compatibility and Mycotoxin Chemotypes among Fusarium graminearum (Gibberella zeae) Isolates from Wheat in Argentina

Identification by PCR of Fusarium culmorum Strains Producing Large and Small Amounts of Deoxynivalenol

Natural occurrence of nivalenol and mycotoxigenic potential of Fusarium graminearum strains in wheat affected by head blight in Argentina

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