Patterns of polymorphism and divergence in the VP1 gene of enterovirus 71 circulating in the Asia-Pacific region between 1994 and 2013

Wu, Junsong; Zhao, Na; Pān, Hào; Wang, Chengmin; Wu, Bin; Zhang, Hongmei; He, Hongxuan; Liú, Dan; Amer, Said; Liu, She-Lan

doi:10.1016/j.jviromet.2013.07.051

Cited by 19 publications

(19 citation statements)

References 63 publications

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“…Such cross-reactive NtAb responses are important for vaccine development. Because serum samples were collected from young children (aged <5 years) in Changchun (northeastern region of China, 2012) and Luoyang (Central Plains region of China, 2008), and EV71-C4a was the major epidemic strain in China in recent years [19], [41], [42], the results suggest that the C4a subgenotype could induce cross-reactive NtAbs against different EV71 subgenotypes in these patients. In screening of the patients' sera with the neutralization assay, we also found that trends in the NtAb titers of Luoyang-294 and -301 against EV71 pseudoviruses of different subgenotypes were more similar to those of the Changchun samples, but different from those of other Luoyang samples.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Cross-Reactive Neutralizing Antibodies in Human HFMD Serum with an EV71 Pseudovirus-Based Assay

Zhang

Liu

et al. 2014

PLoS ONE

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Hand, foot and mouth disease, associated with enterovirus 71 (EV71) infections, has recently become an important public health issue throughout the world. Serum neutralizing antibodies are major indicators of EV71 infection and protective immunity. However, the potential for cross-reactivity of neutralizing antibodies for different EV71 genotypes and subgenotypes is unclear. Here we measured the cross-reactive neutralizing antibody titers against EV71 of different genotypes or subgenotypes in sera collected from EV71-infected children and vaccine-inoculated children in a phase III clinical trial (ClinicalTrials.gov Identifier: NCT01636245) using a new pseudovirus-based neutralization assay. Antibodies induced by EV71-C4a were cross-reactive for different EV71 genotypes, demonstrating that C4a is a good candidate strain for an EV71 vaccine. Our study also demonstrated that this new assay is practical for analyses of clinical samples from epidemiological and vaccine studies.

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Section: Discussionmentioning

confidence: 99%

Analysis of Cross-Reactive Neutralizing Antibodies in Human HFMD Serum with an EV71 Pseudovirus-Based Assay

Zhang

Liu

et al. 2014

PLoS ONE

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“…24 In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory failure was detected at Kantha Bopha Children's Hospital in Phnom Penh, Cambodia. Most of the children who were affected were aged below three years and mainly originated from the southern and central parts of the country.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Duong

Mey

Éloit

et al. 2016

Emerging Microbes & Infections

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Human enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred in young children admitted to the Kantha Bopha Children's Hospital in Phnom Penh, Cambodia. Most died within a day of hospital admission, causing public panic and international concern. In this study, we describe the enterovirus (EV) genotypes that were isolated during the outbreak in 2012 and the following year. From June 2012 to November 2013, 312 specimens were collected from hospitalized and ambulatory patients and tested by generic EV and specific EV-A71 reverse transcription PCR. EV-A71 was detected in 208 clinical specimens while other EVs were found in 32 patients. The VP1 gene and/or the complete genome were generated. Our phylogenetic sequencing analysis demonstrated that 80 EV-A71 strains belonged to the C4a subgenotype and 3 EV-A71 strains belonged to the B5 genotype. Furthermore, some lineages of EV-A71 were found to have appeared in Cambodia following separate introductions from neighboring countries. Nineteen EV A (CV-A6 and CV-A16), 9 EV B (EV-B83, CV-B3, CV-B2, CV-A9, E-31, E-2 and EV-B80) and 4 EV C (EV-C116, EV-C96, CV-A20 and Vaccine-related PV-3) strains were also detected. We found no molecular markers of disease severity. We report here that EV-A71 genotype C4 was the main etiological agent of a large outbreak of HFMD and particularly of severe forms associated with central nervous system infections. The role played by other EVs in the epidemic could not be clearly established.

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“…Other viruses known to cause HFMD include Coxsackie A (CV‐A) viruses (CV‐A2–CV‐A8, CV‐A10–CV‐A12, CV‐A16, and CV‐A20), Coxsackie B viruses (CV‐B1–CV‐B6), and enteric cytopathic human orphan viruses (E3–E6, E9, E11, E18, E25, and E30) [Ni et al, ; Zhang and Xu, ; Cui et al, ; Lin and Xue, ; Qiaoyun et al, ; Yip et al, ; Zhang et al, ,; Chen et al, ]. HFMD is most commonly associated with EV‐A71 and CV‐A16, which thus play an important role in disease outbreaks and progression [Honkanen et al, ; Hyeon et al, ; Li et al, ; Wu et al, ; Zhang et al, ]. Since 2008, HFMD cases and outbreaks caused by CV‐A6 were reported in Singapore [Wu et al, ], Finland [Blomqvist et al, ], Japan [Fujimoto et al, ], Spain [Montes et al, ], and Thailand [Puenpa et al, ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiologic and etiologic characteristics of hand, foot, and mouth disease in Chongqing, China between 2010 and 2013

Lai

Yan

et al. 2015

Journal of Medical Virology

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Hand, foot, and mouth disease (HFMD) has become very common in children, with widespread occurrence across China. The aim of this study was to investigate the epidemiologic and etiologic characteristics of HFMD, including etiologic variations in Chongqing, China. An epidemiologic investigation was based on 3,472 patients who presented with HFMD manifestations and were admitted at the Children's Hospital of Chongqing Medical University between 2010 and 2013. Fecal specimens from 830 patients were analyzed by nested RT-PCR to identify the enterovirus pathogens, and the molecular characterization of HFMD was illustrated by phylogenetic tree analysis. The results of this study indicate that the peak of the HFMD epidemic in Chongqing between 2010 and 2013 occurred between April and July each year. The median age of onset was 2.24 years old, and children under the age of five accounted for 96.4% of all the HFMD cases; the male-to-female ratio was 1.89:1. Enterovirus 71 accounted for a major proportion of the isolated strains every year, including the majority (74%) of severe cases. However, the proportion of Coxsackie A (CV-A) 6 infections increased from 2.11% in 2010 to 16.36% in 2013, while the proportion of CV-A16 infections decreased from 31.23% in 2010 to 4.67% in 2013. Molecular epidemiologic study showed that all enterovirus 71 strains belonged to subgenotype C4a, whereas all CV-A16 strains belonged to genotype B1, including subgenotype B1a and subgenotype B1b.

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Patterns of polymorphism and divergence in the VP1 gene of enterovirus 71 circulating in the Asia-Pacific region between 1994 and 2013

Cited by 19 publications

References 63 publications

Analysis of Cross-Reactive Neutralizing Antibodies in Human HFMD Serum with an EV71 Pseudovirus-Based Assay

Analysis of Cross-Reactive Neutralizing Antibodies in Human HFMD Serum with an EV71 Pseudovirus-Based Assay

Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

Epidemiologic and etiologic characteristics of hand, foot, and mouth disease in Chongqing, China between 2010 and 2013

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