Patterns of SSR variation in bread wheat (Triticum aestivum L.) seeds under ex situ genebank storage and accelerated ageing

Fu, Yong‐Bi; Yang, Mo-Hua; Horbach, Carolee; Kessler, Dallas; Diederichsen, Axel; You, Frank M.; Hong, Wang

doi:10.1007/s10722-015-0349-9

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…The findings reported here, along with those from the SSR analysis [ 29 ], also suggest caution in the search for specific seed aging signals as biomarkers for seed viability prediction. Clearly, selection of a seed aging signal for exploration has become a critical issue.…”

Section: Discussionmentioning

confidence: 58%

“…So far, we have found the associative changes in scutellum nuclear content and morphology with viability loss in naturally aged (NA) and artificially aged (AA) wheat ( Triticum aestivum L.) seeds [ 28 ]. In a separate simple sequence repeat (SSR) study, however, the assayed SSR variability did not show any association with seed viability loss in bread wheat seeds under NA and AA treatments [ 29 ]. This study represents another companion experiment with the aim to assess morphological and molecular changes in NA and AA flax ( Linum usitatissimum L.) seeds using TUNEL assay and DAPI staining and to determine the association of the changes, if any, with seed germination.…”

Section: Introductionmentioning

confidence: 99%

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TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax

Ahmad

Yang

et al. 2018

Plants

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In a search for useful seed aging signals as biomarkers for seed viability prediction, we conducted an experiment using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining to analyze morphological and molecular changes in naturally aged (NA) and artificially aged (AA) flax (Linum usitatissimum L.) seeds. A total of 2546 sections were performed from 112 seeds of 12 NA and AA seed samples with variable germination rates. Analyzing 1384 micrographs generated from TUNEL assay and DAPI staining revealed few alterations of the cellular morphology of the NA and AA seeds. Also, the revealed DNA degradations in the aged flax seeds appeared to be associated with seed samples of low germination rates. These results suggest that oily flax seed aging may alter the cellular morphology differently than starchy wheat seed aging. The results also imply that the TUNEL assay and DAPI staining may not yield informative assessments on cellular alterations and DNA degradation after the aging of oily seeds.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax

Ahmad

Yang

et al. 2018

Plants

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“…Naturally aged (NA) germplasm consisted of six accessions that were stored in the 1990s and had a range of germination rates from 11 to 93%. Their selection was made based on a germination profile of 550 accessions generated from the world wheat germplasm collection conserved in Plant Gene Resources of Canada since the 1970s ( Fu et al, 2015b ). The germination test was conducted following the Association of Official Seed Analysts (1992) guidelines.…”

Section: Methodsmentioning

confidence: 99%

The Associative Changes in Scutellum Nuclear Content and Morphology with Viability Loss of Naturally Aged and Accelerated Aging Wheat (Triticum aestivum) Seeds

Ahmad

Yang

2016

Front. Plant Sci.

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Timely prediction of seed viability loss over long-term storage represents a challenge in management and conservation of ex situ plant genetic resources. However, little attention has been paid to study the process of seed deterioration and seed aging signals under storage. An attempt was made here to investigate morphological and molecular changes in the scutellum and aleurone sections of naturally or artificially aged wheat seeds using TUNEL assay and DAPI staining. Twelve wheat genotypes or samples exposed to natural ageing (NA) or accelerated ageing (AA) were assayed and these samples had germination rates ranging from 11 to 93%. The assayed samples showed substantial changes in scutellum, but not aleurone. The nuclei observed in the majority of the scutellum cells of the NA seed samples of lower germination rates were longer in size and less visible, while the scutellum cell morphology or arrangement remained unchanged. In contrast, longer AA treatments resulted in the loss of scutellum cell structure, collapse of cell layers, and disappearance of honey comb arrangements. These nuclei and structural changes were consistent with the DNA assessments of nuclear alternations for the selected wheat samples. Interestingly, the sample seed germination loss was found to be associated with the reductions in the scutellum nuclear content and with the increases in the scutellum nuclei length to width ratio. These findings are significant for understanding the process of wheat seed deterioration and are also useful for searching for sensitive seed aging signals for developing tools to monitor seed viability under storage.

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“…Aged seeds tend to have reduced seed vigor and could affect agricultural production (Oge et al, 2008;Rajjou and Debeaujon, 2008;Marcos Filho, 2015). In addition, conserving and regenerating about 7.4 million accessions of seed germplasm held in more than 1,750 genebanks around the world are facing technical challenges (FAO, 2010), as standard germination tests are not always effective (Fu et al, 2017). However, our understanding on how seeds age remains limited and effective tools for monitoring seed aging are lacking (Fu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Seed Stored mRNAs are Degraded Constantly over Aging Time, as Revealed by New Quantification Methods

Zhao

Wang

et al. 2020

Front. Plant Sci.

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How plant seeds age remains poorly understood and effective tools for monitoring seed aging are lacking. Dry seeds contain various stored mRNAs which are believed to be required for protein synthesis during early stages of seed germination. We reasoned that seed stored mRNAs would undergo degradation during seed aging, based on the propensity of mRNAs to degrade. We performed RT-PCR and qPCR analyses to study the changes in stored mRNA levels of Arabidopsis seeds during aging. All stored mRNAs analyzed were gradually degraded in both naturally and artificially aged seeds. The difference in Ct values between aged and control seeds (DCt value) was highly correlated with the mRNA fragment size and seed aging time. We derived mathematical equations for estimating the relative amount of undamaged stored mRNAs and frequency of the breakdown at one nucleotide level for individual mRNAs. Stored mRNAs were found to break down randomly. The frequency of breaks per nucleotide per day, which we named b value, remained fairly constant under the same aging conditions over aging time. This parameter should allow the effects of different conditions on the degradation of stored mRNAs to be quantitatively compared. Also, we showed that the change in stored mRNA levels could serve as a more precise biomarker for seed aging assessment than three existing methods. These methods and findings will advance the studies of stored mRNAs and seed ageing in plants, and likely slow RNA degradation in non-plant systems.

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Patterns of SSR variation in bread wheat (Triticum aestivum L.) seeds under ex situ genebank storage and accelerated ageing

Cited by 11 publications

References 29 publications

TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax

TUNEL Assay and DAPI Staining Revealed Few Alterations of Cellular Morphology in Naturally and Artificially Aged Seeds of Cultivated Flax

The Associative Changes in Scutellum Nuclear Content and Morphology with Viability Loss of Naturally Aged and Accelerated Aging Wheat (Triticum aestivum) Seeds

Arabidopsis Seed Stored mRNAs are Degraded Constantly over Aging Time, as Revealed by New Quantification Methods

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