Patulin Detoxification by Recombinant Manganese Peroxidase from Moniliophthora roreri Expressed by Pichia pastoris

Abstract

The fungal secondary metabolite patulin is a mycotoxin widespread in foods and beverages which poses a serious threat to human health. However, no enzyme was known to be able to degrade this mycotoxin. For the first time, we discovered that a manganese peroxidase (MrMnP) from Moniliophthora roreri can efficiently degrade patulin. The MrMnP gene was cloned into pPICZα(A) and then the recombinant plasmid was transformed into Pichia pastoris X-33. The recombinant strain produced extracellular manganese peroxidase with an activity of up to 3659.5 U/L. The manganese peroxidase MrMnP was able to rapidly degrade patulin, with hydroascladiol appearing as a main degradation product. Five mg/L of pure patulin were completely degraded within 5 h. Moreover, up to 95% of the toxin was eliminated in a simulated patulin-contaminated apple juice after 24 h. Using Escherichia coli as a model, it was demonstrated that the deconstruction of patulin led to detoxification. Collectively, these traits make MrMnP an intriguing candidate useful in enzymatic detoxification of patulin in foods and beverages.

Exploring the cellulolytic and hemicellulolytic activities of manganese peroxidase for lignocellulose deconstruction

“…In this study, the Mr MnP, expressed in P . pastoris as before [ 29 ], was purified using hydrophobic-interaction chromatography (Fig. 1 a).…”

“…Most studies of MnPs focus on their ability to biodegrade organic pollutants, toxins, etc. This study explored its ability in LCB decomposition by using an MnP from Moniliophthora roreri , Mr MnP, distinguished from other enzyme counterparts by its high-level secretory expression with a strong potential application prospect [ 28 , 29 ]. The effects of buffer components, pH, and H 2 O 2 on Mr MnP activity were first examined.…”

Bioprotective yeasts: Potential to limit postharvest spoilage and to extend shelf life or improve microbial safety of processed foods

“…Additionally, it entails the elicitation of systemic resistance within host plant organisms [ 36 ]. In addition, numerous studies have demonstrated that some yeast strains possess detoxification functions, converting toxins produced by fungi into non-toxic or low-toxic substances to defend against fungal attack [ 37 ]. Various attempts have been made to improve the effectiveness of antagonistic yeasts against harmful microorganisms by increasing their viability or by combining them with other physical or chemical means.…”

Modulation of extracellular Penicillium expansum-driven acidification by Papiliotrema terrestris affects biosynthesis of patulin and has a possible role in biocontrol activity

“…The prevention of PAT contamination is mainly based on controlling P. expansum through the reduction of conidial germination, germ tube elongation and inhibition of fungal growth, with a consequent decrease of PAT biosynthesis and accumulation ( Yu et al, 2020 ). Conversely, PAT degradation consists of enzymatic degradation operated by several bacterial and yeast species leading to the formation of the end-products desoxypatulinic acid, (Z)-ascladiol, and hydroascladiol ( Castoria et al, 2011 ; Ianiri et al, 2013 ; Mahunu, 2017 ; Tannous et al, 2017 ; Wei et al, 2020 ; Awuchi et al, 2021 ; Wang et al, 2022 ). Other studies show that some yeast species are also able to decrease PAT concentration through its adsorption to the cell wall ( Caridi, 2007 ; McCormick, 2013 ; Nešić et al, 2021 ).…”