Paul trapping of charged particles in aqueous solution

Guan, Weihua; Joseph, Sony; Park, Jae Hyun; Krstić, Predrag; Reed, Mark A.

doi:10.1073/pnas.1100977108

Cited by 51 publications

(61 citation statements)

References 30 publications

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“…Recently, Guan et al [1, 2] have showed the feasibility of an aqueous , radio-frequency (rf, AC) quadrupole micro-trap for localization and control of charged micro-particles. This is a remarkable extension of the applicability of the quadrupole Paul micro-trap [3, 4] in addition to its conventional applications in mass spectrometry [5], quantum information processing [6, 7], microdynamical sensors [8], etc.…”

Section: Introductionmentioning

confidence: 99%

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Stability of an aqueous quadrupole micro-trap

Park¹,

Krstić²

2012

J. Phys.: Condens. Matter

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Recently demonstrated functionality of an aqueous quadrupole micro- or nano-trap opens a new avenue for applications of the Paul traps, like is confinement of a charged biomolecule which requires water environment for its chemical stability. Besides strong viscosity forces, motion of a charged particle in the aqueous trap is subject to dielectrophoretic and electrophoretic forces. In this study, we describe the general conditions for stability of a charged particle in an aqueous quadrupole trap. We find that for the typical micro-trap parameters, effects of both dielectrophoresis and electrophoresis significantly influence the trap stability. In particular, the aqueous quadrupole trap could play of a role of a synthetic virtual nanopore for the 3rd generation of DNA sequencing technology.

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Section: Introductionmentioning

confidence: 99%

“…Considering that typical biomolecules (e.g. proteins) are stable in water and have a considerable amount of charge [9, 10], this novel aqueous quadrupole micro-trap is an excellent candidate for localization and manipulation of their motion in a confinement region of space [1, 2, 11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Stability of an aqueous quadrupole micro-trap

Park¹,

Krstić²

2012

J. Phys.: Condens. Matter

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“…Alternatively, trapping multiple cells (or particles) for shorter durations could be achieved by alternating the polarity of the compressional and extensional flow axes, analogous to implementing a Paul trap in aqueous solution. 55 In addition, trapping techniques with higher temporal resolution may require decoupling image acquisition with centroid detection by implementing hardware trapping for improved feedback latency as reported in methods such as optical 27 and electrophoretic techniques. 38 …”

Section: Discussionmentioning

confidence: 99%

Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

et al. 2014

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We report an automated microfluidic-based platform for single cell analysis that allows for cell culture in free solution with the ability to control the cell growth environment. Using this approach, cells are confined by the sole action of gentle fluid flow, thereby enabling non-perturbative analysis of cell growth away from solid boundaries. In addition, the single cell microbioreactor allows for precise and time-dependent control over cell culture media, with the combined ability to observe the dynamics of non-adherent cells over long time scales. As a proof-of-principle demonstration, we used the platform to observe dynamic cell growth, gene expression, and intracellular diffusion of repressor proteins while precisely tuning the cell growth environment. Overall, this microfluidic approach enables the direct observation of cellular dynamics with exquisite control over environmental conditions, which will be useful for quantifying the behaviour of single cells in well-defined media.

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“…However, confinement and fine-scale manipulation of macromolecules and nanoparticles remains a significant challenge. Currently, particle trapping methods based on acoustic [1][2][3][4] , electrokinetic [5][6][7][8][9][10][11][12][13][14][15] , magnetic [16][17][18] , and optical [19][20][21][22][23][24][25][26][27][28] fields are utilized, but these methods are limited to trapping particles with specific material properties and bulky micron-scale dimensions. [29][30][31] Recently, we developed a new flow-based confinement method that enables 2-D manipulation of single micro and nanoscale particles suspended in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Confinement of single macromolecules in free solution using a hydrodynamic trap

Tanyeri

2014

SPIE Proceedings

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We demonstrate the feasibility of trapping and manipulating individual macromolecules such as globular proteins (~ 5 nm in diameter) in free solution using a flow-based, microfluidic confinement method. This new method enables confinement of small nanoscale objects in free solution by utilizing a planar extensional flow created at a microchannel junction. The fluid flow based confinement method described in this work expands the micro/nanomanipulation toolbox by offering a powerful and versatile platform for non-perturbative observation and analysis of single macromolecules in free solution without force fields (electrical, magnetic, optical and acoustic) and surface immobilization.

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Paul trapping of charged particles in aqueous solution

Cited by 51 publications

References 30 publications

Stability of an aqueous quadrupole micro-trap

Stability of an aqueous quadrupole micro-trap

Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

Confinement of single macromolecules in free solution using a hydrodynamic trap

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