Paw Test with Syngeneic Tumor Cells in Inbred Mice Bearing a Transplanted Sarcoma

Wells, James H.; Cain, William A.; Bozalis, J.R.; Wells, R.S.; Flora, J.D.

doi:10.1159/000231218

Cited by 4 publications

(11 citation statements)

References 5 publications

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“…All paw tests were performed as previously described [19]. In brief summary, the thickness of a rear paw was measured, 0.05 ml of test materi al was injected superficially into the footpad, and the paw thickness was remeasured after a suitable reaction time.…”

Section: Phytohemagglutinin (Ph A)mentioning

confidence: 99%

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Suppression of Tuberculin and Phytohemagglutinin Skin Tests by Large Tumors in Inbred Mice

Wells¹,

Cain²,

Wells³

et al. 1974

Int Arch Allergy Immunol

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Suppression of PPHA and PPD paw test was observed in mice with a transplantable methylcholanthrene-induced sarcoma. Serum from tumor-bearing mice contained a factor which suppressed paw test reactivity in normal mice. Less suppression of paw test was seen in mice previously injected with complete Freund adjuvant. It is suggested that these observations form the basis of an experimental model for study of tumor-associated cutaneous anergy.

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Section: Phytohemagglutinin (Ph A)mentioning

confidence: 99%

“…We have reported studies with two antigenically distinct methylchol anthrene-induced sarcomas in inbred C3H mice [19]. Viable tumor cells were used to test for specific cutaneous reactivity (paw test) to tumor an-tigens in suitably immunized mice.…”

Section: Introductionmentioning

confidence: 99%

Suppression of Tuberculin and Phytohemagglutinin Skin Tests by Large Tumors in Inbred Mice

Wells¹,

Cain²,

Wells³

et al. 1974

Int Arch Allergy Immunol

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“…As the growing tumors became larger, paw reactiv ity to tumor cells, other antigen, and PHA became less reactive. Serum from mice with large tumors suppressed paw reactivity of normal mice to PHA [10,11],…”

Section: Introductionmentioning

confidence: 95%

“…Induction and serial transplantation of the MC-17 tumor have been previously described [10,11]. The MC-16 tumor was a similarly induced and transplanted sarcoma which did not crossreact with the MC-17 tumor by paw test.…”

Section: Tumorsmentioning

confidence: 99%

“…The footpad assay, or paw test, is a skin test procedure which uses paw swelling, af ter local injection of antigen into a footpad of sensitized animals, as an index of specific immunological reactivity [3,5,10,11], A very similar test can be performed with phy tohemagglutinin [11], Paw swelling after injection of PHA in normal or tumor-bear ing mice reflects, at least in part, T cell function.…”

Section: Introductionmentioning

confidence: 99%

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Studies of the Paw Test and Tumor-Associated Immunosuppression in Mice

Wells¹,

Pollack²,

Cox³

et al. 1978

Int Arch Allergy Immunol

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Specific paw test reactivity to tumor cells was transferred to normal mice using lymphoid cells or serum from mice with a growing or surgically excised methyl-cholanthrene-induced transplantable sarcoma. When the donors had been sensitized to PPD and to tumor, normal recipients of lymphoid cells became reactive by specific paw test to tumor cells and PPD. Normal recipients of serum from the same donors also became reactive to tumor cells. Normal recipients of serum from tumor-bearing mice developed depressed PHA paw tests. Adoptive transfer with cells or serum of paw test reactivity to tumor and PPD was unsuccessful when recipients had large tumors. This was the case when the recipients had either tumor of the same line or that of a separate line that did not cross-react antigenically.

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Ability of delayed‐type hypersensitivity reactions to distinguish tumor‐associated antigens and histocompatibility antigens in soluble extracts from murine fibrosarcomas

Kon

Forbes

Klein

1976

Intl Journal of Cancer

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The footpad swelling (FPS) test for delayed-type hypersensitivity in the mouse was evaluated for its ability to measure both tumor-associated antigens (TAA) and histocompatibility (H) antigens solubilized from methylcholanthrene (MCA) induced fibrosarcomas of C57B1/6 (B6) mice. Tests for TAA were performed in mice immune to syngeneic tumors while H-antigens were assayed in mice immunized with skin allografts. FPS was most intense in B6 mice challenged with TAA from the immunizing B6 tumor, but also occurred in response to cross-reactive TAA solubilized from another B6 fibrosarcoma. Tests for tumor-associated H-antigens in allograft immune mice were strongly positive in response to donor/recipient H-antigen differences and proved sensitive to shared third-party H-antigen differences. Comparison of soluble antigens from the same tumor maintained in vitro and in vivo revealed that, while both TAA and H-antigens could be detected in preparations from the in vivo tumor line, only TAA and not H-antigens could be detected by RPS in extracts prepared from the in vitro tumor line. These experiments have demonstrated that the mouse FPS test can distinguish both TAA and H-antigen specificities persent in the same complex mixture of tumor-cell antigens.

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Paw Test with Syngeneic Tumor Cells in Inbred Mice Bearing a Transplanted Sarcoma

Cited by 4 publications

References 5 publications

Suppression of Tuberculin and Phytohemagglutinin Skin Tests by Large Tumors in Inbred Mice

Suppression of Tuberculin and Phytohemagglutinin Skin Tests by Large Tumors in Inbred Mice

Studies of the Paw Test and Tumor-Associated Immunosuppression in Mice

Ability of delayed‐type hypersensitivity reactions to distinguish tumor‐associated antigens and histocompatibility antigens in soluble extracts from murine fibrosarcomas

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