Pax-6 Is First Expressed in a Region of Ectoderm Anterior to the Early Neural Plate: Implications for Stepwise Determination of the Lens

Li, Huashun; Yang, Jun-Ming; Jacobson, Richard D.; Pasko, Dean; Sundin, Olof

doi:10.1006/dbio.1994.1077

Cited by 269 publications

(166 citation statements)

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“…Out of the transcription factors examined, mesotheliomas showed activated DLX5, 16 ONECUT1 (HNF6), 17 and Pax6, 18 whereas the activation of HAND1, 19 ISL1, 20 and MEIS1 21 were not observed.…”

Section: Expression Of Mesoderm-specific Transcription Factorsmentioning

confidence: 98%

Homozygous deletion of CDKN2A/2B is a hallmark of iron-induced high-grade rat mesothelioma

Akatsuka

Yamashita

et al. 2010

Laboratory Investigation

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In humans, mesothelioma has been linked to asbestos exposure, especially crocidolite and amosite asbestos, which contain high amounts of iron. Previously, we established a rat model of iron-induced peritoneal mesothelioma with repeated intraperitoneal injections of iron saccharate and an iron chelator, nitrilotriacetate. Here, we analyze these mesotheliomas using array-based comparative genomic hybridization (aCGH) and gene expression profiling by microarray. Mesotheliomas were classified into two distinct types after pathologic evaluation by immunohistochemistry. The major type, epithelioid mesothelioma (EM), originated in the vicinity of tunica vaginalis testis, expanded into the upper peritoneal cavity and exhibited papillary growth and intense podoplanin immunopositivity. The minor type, sarcomatoid mesothelioma (SM), originated from intraperitoneal organs and exhibited prominent invasiveness and lethality. Both mesothelioma types showed male preponderance. SMs revealed massive genomic alterations after aCGH analysis, including homozygous deletion of CDKN2A/2B and amplification of ERBB2 containing region, whereas EMs showed less genomic alterations. Uromodulin was highly expressed in most of the cases. After 4-week treatment, iron deposition in the mesothelia was observed with 8-hydroxy-2 0 -deoxyguanosine formation. These results not only show two distinct molecular pathways for iron-induced peritoneal mesothelioma, but also support the hypothesis that oxidative stress by iron overload is a major cause of CDKN2A/2B homozygous deletion.

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Section: Expression Of Mesoderm-specific Transcription Factorsmentioning

confidence: 98%

Homozygous deletion of CDKN2A/2B is a hallmark of iron-induced high-grade rat mesothelioma

Akatsuka

Yamashita

et al. 2010

Laboratory Investigation

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“…The earliest transcription factor expressed when embryonic SCs are destined to the keratinocyte lineage is p63 [111]. However, the first recognized ectodermal gene expression associated with eye development is PAX 6 [112], which is consistently turned on by the ocular surface epithelium postnatally [113]. In contrast, head surface ectodermal cells lacking PAX 6 expression continue a "default differentiation pathway" to generate the epidermis.…”

Section: When and How Does Limbal Stem Cell Niche Form During Morphogmentioning

confidence: 99%

Niche regulation of corneal epithelial stem cells at the limbus

et al. 2007

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Among all adult somatic stem cells, those of the corneal epithelium are unique in their exclusive location in a defined limbal structure termed Palisades of Vogt. As a result, surgical engraftment of limbal epithelial stem cells with or without ex vivo expansion has long been practiced to restore sights in patients inflicted with limbal stem cell deficiency. Nevertheless, compared to other stem cell examples, relatively little is known about the limbal niche, which is believed to play a pivotal role in regulating self-renewal and fate decision of limbal epithelial stem cells. This review summarizes relevant literature and formulates several key questions to guide future research into better understanding of the pathogenesis of limbal stem cell deficiency and further improvement of the tissue engineering of the corneal epithelium by focusing on the limbal niche.

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“…By examining the expression of several different genes during embryonic lens formation in Xenopus, one can evaluate the present model of lens induction and attempt to define tissue properties in a molecular context. Some experimental data indicate that changes in Pax-6 expression can be correlated with developmental changes in lens-forming competence in embryonic head ectoderm in the chicken (Li et al, 1994). In a recent study, Zygar et al (1998) demonstrated that certain changes in gene expression are triggered by specific phases of lens development in Xenopus.…”

Section: Examining the Model Of Embryonic Lens Inductionmentioning

confidence: 99%

Characterizing gene expression during lens formation in Xenopus laevis: Evaluating the model for embryonic lens induction

Henry

Carinato

Schaefer

et al. 2002

Developmental Dynamics

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Few directed searches have been undertaken to identify the genes involved in vertebrate lens formation. In the frog Xenopus, the larval cornea can undergo a process of transdifferentiation to form a new lens once the original lens is removed. Based on preliminary evidence, we have shown that this process shares many elements of a common molecular/genetic pathway to that involved in embryonic lens development. A subtracted cDNA library, enriched for genes expressed during cornea-lens transdifferentiation, was prepared. The similarities/identities of specific clones isolated from the subtracted cDNA library define an expression profile of cells undergoing cornea-lens transdifferentiation ("lens regeneration") and corneal wound healing (the latter representing a consequence of the surgery required to trigger transdifferentiation). Screens were undertaken to search for genes expressed during both transdifferentiation and embryonic lens development. Significantly, new genes were recovered that are also expressed during embryonic lens development. The expression of these genes, as well as others known to be expressed during embryonic development in Xenopus, can be correlated with different periods of embryonic lens induction and development, in an attempt to define these events in a molecular context. This information is considered in light of our current working model of embryonic lens induction, in which specific tissue properties and phases of induction have been previously defined in an experimental context. Expression data reveal the existence of further levels of complexity in this process and suggests that individual phases of lens induction and specific tissue properties are not strictly characterized or defined by expression of individual genes.

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Pax-6 Is First Expressed in a Region of Ectoderm Anterior to the Early Neural Plate: Implications for Stepwise Determination of the Lens

Cited by 269 publications

References 0 publications

Homozygous deletion of CDKN2A/2B is a hallmark of iron-induced high-grade rat mesothelioma

Homozygous deletion of CDKN2A/2B is a hallmark of iron-induced high-grade rat mesothelioma

Niche regulation of corneal epithelial stem cells at the limbus

Characterizing gene expression during lens formation in Xenopus laevis: Evaluating the model for embryonic lens induction

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