Pax6: A multi-level regulator of ocular development

Shaham, Ohad; Menuchin, Yotam; Farhy, Chen; Ashery‐Padan, Ruth

doi:10.1016/j.preteyeres.2012.04.002

Cited by 198 publications

(191 citation statements)

References 370 publications

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“…Paired box 6 (PAX6) is a highly conserved transcription regulatory factor involved in multiple biological processes, including embryogenesis, cell differentiation, biosynthesis and cell motility (1,2). Accumulating evidence has demonstrated that PAX6 is crucial in the development of the central nervous system (3).…”

Section: Introductionmentioning

confidence: 99%

PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

Cheng

Cao²,

Chen

et al. 2014

Molecular Medicine Reports

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Abstract. Paired box 6 (PAX6), a highly conserved transcription factor, is important in glioma. However, the molecular mechanisms involved remain unclear. The present study demonstrated that the expression of PAX6 was significantly reduced with the malignancy of glioma and also identified PAX6 as a novel target of microRNA (miR)-335, which was significantly upregulated in glioma. The inhibition of miR-335 increased the protein expression of PAX6, whereas the upregulation of miR-335 suppressed its expression in human glioma U251 and U87 cells. Furthermore, upregulation of miR-335 promoted U251 cell proliferation, colony formation and invasion, which was reversed by the overexpression of PAX6. Furthermore, the present study demonstrated that the effect of miR-335 on U251 cell invasion was via the modulation of matrix metalloproteinase (MMP)-2 and MMP-9 expression by targeting PAX6. In conclusion, the present study demonstrated that PAX6, as a novel target of miR-335, has an anti-oncogenic function in glioma, and thus PAX6 may serve as a therapeutic target for glioma.

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Section: Introductionmentioning

confidence: 99%

PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells

Cheng

Cao²,

Chen

et al. 2014

Molecular Medicine Reports

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“…Thus, the essential role of Pax6 in the CNS is highly context dependent. Pax6 also plays an important role as a multilevel regulator of ocular development [17]. In the retina, Pax6 directly controls the transcriptional activation of retinogenic bHLH factors and mediates the full retinogenic potential of retinal progenitor cells [39].…”

Section: Discussionmentioning

confidence: 99%

“…The transcription factor Pax6 is highly conserved among vertebrates and is essential for a wide variety of developmental processes within the central nervous system (CNS), sensory organs, and the endocrine system [15][16][17][18][19]. Homozygous Pax6 mutant mice (Pax6…”

Section: Introductionmentioning

confidence: 99%

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Suzuki

Sakurai

Yamazaki

et al. 2015

Stem Cells and Development

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In the mammalian olfactory epithelium (OE), olfactory receptor neurons (ORNs) are continuously regenerated throughout the animal's lifetime. Horizontal basal cells (HBCs) in the OE express the epithelial marker keratin 5 (K5) and the stem cell marker Pax6 and are considered relatively quiescent tissue stem cells in the OE. Pax6 is a key regulator of several developmental processes in the central nervous system and in sensory organs. Although Pax6 is expressed in the OE, its precise role remains unknown, particularly with respect to stem celllike HBCs. To investigate the function of Pax6 in the developmental and regenerative processes in the OE, we generated conditional Pax6-knockout mice carrying a loxP-floxed Pax6 gene. Homozygous Pax6-floxed mice were crossed with K5-Cre transgenic mice to generate HBC-specific Pax6-knockout (Pax6-cKO) mice. We confirmed that the deletion of Pax6 expression in HBCs was sufficiently achieved in zone 1 of the OE in Pax6-cKO mice 3 days after methimazole-induced severe damage. In this condition, regeneration of the OE was dramatically impaired; both OE thickness and the number of ORNs were significantly decreased in the regenerated OE of Pax6-cKO mice. These results suggest that Pax6 expression is essential for HBCs to differentiate into neuronal cells during the regeneration process following severe injury.

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“…PAX6 es conocido como un «gen maestro» del desarrollo ocular, ya que interviene en etapas tempranas y finales de la morfogénesis ocular, la diferenciación neural y la configuración de conexiones sinápticas 52,53 . PAX6 se encuentra ubicado en el cromosoma 11p13 54,55 y se expresa ampliamente en la vesícula óptica, el cristalino, la retina y la córnea durante el desarrollo ocular [56][57][58] .…”

Section: Pax6unclassified