Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration

Sambasivan, Ramkumar; Yao, Roseline; Kissenpfennig, Adrien; Wittenberghe, Laetitia van; Páldi, Andràs; Gayraud-Morel, Barbara; Guénou, Hind; Malissen, Bernard; Tajbakhsh, Shahragim; Galy, Anne

doi:10.1242/dev.067587

Cited by 777 publications

(542 citation statements)

References 56 publications

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“…These results are not in contradiction with those reported here for the following reasons: the ablation of Pax7 + satellite cells was carried out in adult mice, at an age when the contribution of AP + pericyets to muscle regeneration becomes negligible. It is also possible that satellite cells are required to induce other cell types to adopt a myogenic fate, as suggested by Sambasivan et al 48 In conclusion, we have demonstrated that vascular pericytes, resident in skeletal muscle, are bi-potent (as confirmed by clonal analysis), as they contribute to both the smooth muscle layer of blood vessels and skeletal muscle fibre development, including the satellite cell pool, during unperturbed, postnatal growth of the mouse. It is still unknown how this choice is regulated in vivo, although it is plausible that signals emanating from neighbour muscle fibres or endothelium may direct the cells to a skeletal or to a smooth muscle fate, respectively.…”

Section: Discussionsupporting

confidence: 81%

“…Very recently, three reports [46][47][48] showed that selective ablation of Pax7-expressing satellite cells during adult life permanently hampers subsequent attempts to regenerate skeletal muscles, leading to the conclusion that no other endogenous cell types may substitute for satellite cells. These results are not in contradiction with those reported here for the following reasons: the ablation of Pax7 + satellite cells was carried out in adult mice, at an age when the contribution of AP + pericyets to muscle regeneration becomes negligible.…”

Section: Discussionmentioning

confidence: 99%

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Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells

Dellavalle¹,

Maroli

Covarello³

et al. 2011

Nat Commun

404

376

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skeletal muscle fibres form by fusion of mesoderm progenitors called myoblasts. After birth, muscle fibres do not increase in number but continue to grow in size because of fusion of satellite cells, the postnatal myogenic cells, responsible for muscle growth and regeneration. numerous studies suggest that, on transplantation, non-myogenic cells also may contribute to muscle regeneration. However, there is currently no evidence that such a contribution represents a natural developmental option of these non-myogenic cells, rather than a consequence of experimental manipulation resulting in cell fusion. Here we show that pericytes, transgenically labelled with an inducible Alkaline Phosphatase CreERT2, but not endothelial cells, fuse with developing myofibres and enter the satellite cell compartment during unperturbed postnatal development. This contribution increases significantly during acute injury or in chronically regenerating dystrophic muscle. These data show that pericytes, resident in small vessels of skeletal muscle, contribute to its growth and regeneration during postnatal life.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells

Dellavalle¹,

Maroli

Covarello³

et al. 2011

Nat Commun

404

376

View full text Add to dashboard Cite

show abstract

“…Satellite cells are specialized myogenic stem cells that are located beneath the basement membranes of muscle fibers and are required for muscle regeneration and growth (Sambasivan et al, 2011). In response to injury, satellite cells exit their quiescent state and then proliferate, differentiate, and fuse with existing myofibers to provide new myonuclei and replace or repair injured cells (Relaix & Zammit, 2012).…”

Section: Introductionmentioning

confidence: 99%

Myeloid cell‐derived tumor necrosis factor‐alpha promotes sarcopenia and regulates muscle cell fusion with aging muscle fibers

et al. 2018

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Sarcopenia is age‐related muscle wasting that lacks effective therapeutic interventions. We found that systemic ablation of tumor necrosis factor‐α (TNF‐α) prevented sarcopenia and prevented age‐related change in muscle fiber phenotype. Furthermore, TNF‐α ablation reduced the number of satellite cells in aging muscle and promoted muscle cell fusion in vivo and in vitro. Because CD68+ macrophages are important sources of TNF‐α and the number of CD68+ macrophages increases in aging muscle, we tested whether macrophage‐derived TNF‐α affects myogenesis. Media conditioned by TNF‐α‐null macrophages increased muscle cell fusion in vitro, compared to media conditioned by wild‐type macrophages. In addition, transplantation of bone marrow cells from wild‐type mice into TNF‐α‐null recipients increased satellite cell numbers and reduced numbers of centrally nucleated myofibers, indicating that myeloid cell‐secreted TNF‐α reduces muscle cell fusion. Transplanting bone marrow cells from wild‐type mice into TNF‐α‐null recipients also increased sarcopenia, although transplantation did not restore the age‐related change in muscle fiber phenotype. Collectively, we show that myeloid cell‐derived TNF‐α contributes to muscle aging by affecting sarcopenia and muscle cell fusion with aging muscle fibers. Our findings also show that TNF‐α that is intrinsic to muscle and TNF‐α secreted by immune cells work together to influence muscle aging.

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“…satellite cells) located between the basal lamina and sarcolemma (Mauro, 1961). Resident satellite cells normally exist in a mitotically quiescent state in adult muscle, but re-enter the myogenic program in response to regenerative cues such as mechanical loading, injury, or disease (Lepper et al, 2011;Sambasivan et al, 2011). Activated satellite cells may then proliferate nominally to repair small localized damage, fuse together to form myofibers in the face of considerable muscle injury, or provide additional nuclei to support myofiber expansion (Adams, 2006;Kook et al, 2006;Le Grand and Rudnicki, 2007).…”

Section: Introductionmentioning

confidence: 99%

β-Hydroxy-β-Methylbutyrate Did Not Enhance High Intensity Resistance Training-Induced Improvements in Myofiber Dimensions and Myogenic Capacity in Aged Female Rats

Kim

Park

Lee

et al. 2012

Molecules and Cells

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Older women exhibit blunted skeletal muscle hypertrophy following resistance training (RT) compared to other age and gender cohorts that is partially due to an impaired regenerative capacity. In the present study, we examined whether β-hydroxy-β-methylbutyrate (HMB) provision to aged female rodents would enhance regenerative mechanisms and facilitate RT-induced myofiber growth. Nineteen-month old female Sprague-Dawley rats were randomly divided into three groups: HMB (0.48 g/kg/d; n = 6), non-HMB (n = 6), and control (n = 4). HMB and non-HMB groups underwent RT every third day for 10 weeks using a ladder climbing apparatus. Whole body strength, grip strength, and body composition was evaluated before and after RT. The gastrocnemius and soleus muscles were analyzed using magnetic resonance diffusion tensor imaging, RT-PCR, and immunohistochemistry to determine myofiber dimensions, transcript expression, and satellite cells/myonuclei, respectively. ANOVAs were used with significance set at p < 0.05. There were significant time effects (pre vs. post) for whole body strength (+262%), grip strength (+17%), lean mass (+20%), and fat mass (-19%). Both RT groups exhibited significant increases in the mean myofiber cross-sectional area (CSA) in the gastrocnemius and soleus (+8-22%) compared to control. Moreover, both groups demonstrated significant increases in the numbers of satellite cells (+100-108%) and myonuclei (+32%) in the soleus but not the gastrocnemius. A significant IGF-I mRNA elevation was only observed in soleus of the HMB group (+33%) whereas MGF and myogenin increased significantly in both groups (+32-40%). Our findings suggest that HMB did not further enhance intense RT-mediated myogenic mechanisms and myofiber CSA in aged female rats.

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Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration

Abstract: There was an error published in Development 138, 3647-3656.The panel labels on the left indicating genotypes were misaligned in Fig. 5A. The corrected Fig. 5 appears in full below.The authors apologise to readers for this mistake.

Cited by 777 publications

References 56 publications

Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells

Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells

Myeloid cell‐derived tumor necrosis factor‐alpha promotes sarcopenia and regulates muscle cell fusion with aging muscle fibers

β-Hydroxy-β-Methylbutyrate Did Not Enhance High Intensity Resistance Training-Induced Improvements in Myofiber Dimensions and Myogenic Capacity in Aged Female Rats

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