PBMCs to Stress-Associated miR-18a-5p and miR-22-3p Ratios as New Indicators of Metabolic Syndrome

Huang, Yuxiang; Zhao, Lijun; Yan, Yuxiang; Chen, Jingyu; Liu, Pengfei; Xv, Weicheng; Ge, Qian; Li, Chijian; Liang, Shiyi; Zou, Hequn; Li, Yongqiang

doi:10.1155/2020/8159342

Cited by 7 publications

(7 citation statements)

References 24 publications

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“… 14 , 15 miR-18a-5p can target the glucocorticoid receptor, which is involved in the regulation of inflammatory response and related physiological events. 29 In the current study, the gain and loss function experiments revealed that upregulation of miR-18a-5p aggravated CSE-induced cell apoptosis and inflammatory cytokines release. Moreover, miR-18a-5p overexpression reversed the influence of CASC2 on cell apoptosis and inflammation in 16HBE cells.…”

Section: Discussionmentioning

confidence: 51%

LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis

Zhang

Zeng

et al. 2021

Ther Adv Respir Dis

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Aims: Chronic obstructive pulmonary disease (COPD) is a systemic disease. Several long non-coding RNAs (lncRNAs) have been identified to be aberrantly expressed in COPD patients. This study investigated the role of lncRNA cancer susceptibility candidate 2 (CASC2) in COPD, as well as its potential mechanism. Methods: Fifty smokers with COPD and another 50 smokers without COPD were recruited. Receiver operating characteristic curve was constructed to assess the diagnostic value of CASC2 in COPD patients. 16HBE cells were treated with cigarette smoke extract (CSE) to establish a cell model. qRT-PCR was used for the measurement of mRNA levels. The cell viability and apoptosis were detected by using Cell Counting Kit-8 and flow cytometry assay. Enzyme-linked immunosorbent assay was performed to detect the levels of proinflammatory cytokines. Luciferase reporter assay was performed for the target gene analysis. Results: Serum CASC2 was dramatically decreased in COPD patients compared with smokers without COPD, and was positively associated with FEV1 (forced expiratory volume in one second). Serum CASC2 was overexpressed in severe COPD patients, and had the diagnostic accuracy to distinguish COPD patients from smokers. CASC2 overexpression alleviated CSE-induced apoptosis and inflammation in 16HBE cells. CASC2 functions as a ceRNA of miR-18a-5p. Upregulation of miR-18a-5p reversed the influence of CASC2 on cell apoptosis and inflammation in 16HBE cells. IGF1 was the target gene of miR-18a-5p. Conclusion: CASC2 was downregulated in COPD patients and it might be a promising biomarker for the disease diagnosis. Overexpression of CASC2 might inhibit the bronchial epithelial cell apoptosis and inflammation via targeting miR-18a-5p/IGF1 axis. The reviews of this paper are available via the supplemental material section.

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Section: Discussionmentioning

confidence: 51%

LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis

Zhang

Zeng

et al. 2021

Ther Adv Respir Dis

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“…The detailed characteristics of the selected studies are shown in Table S2, Supplemental Digital Content, http://links.lww.com/MD/L813. Thirty-seven studies [5,11–46] defined MetS according to the third report of the National Cholesterol Education Program-Adult Treatment Panel. [47] Fifteen studies [6,48–61] assessed metabolic syndrome using the International Diabetes Federation criteria.…”

Section: Resultsmentioning

confidence: 99%

Association of white blood cell parameters with metabolic syndrome: A systematic review and meta-analysis of 168,000 patients

Mahmood,

Haider,

Samad

et al. 2024

Medicine

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Background: Leukocyte parameters are predicted to be affected in patients with metabolic syndrome (MetS). We conducted a systematic review and meta-analysis to study the association between white blood cell parameters (WBC) in people with and without MetS. Methods: PubMed, EMBASE, Scopus and Cochrane Library databases were searched according to the study protocol. The standardized mean difference (SMD) and 95% confidence intervals (CI) of leukocyte markers between individuals with and without MetS were pooled using an inverse variance model. Additionally, a subgroup analysis by sex was performed where possible. Methodological quality assessment was conducted using the Newcastle-Ottawa scale (NOS) for observational studies and the Cochrane Risk of Bias tool 2.0 for Randomized Controlled Trials (RCTs). Results: Of 6068 articles identified, 63 were eligible for the study. Compared to controls, individuals with MetS showed significantly higher concentrations of total leukocyte count (SMD [95% CI]: 0.60 [0.55–0.65]; P < .00001; I 2 = 100%), neutrophil counts (0.32 [0.28–0.37]; P < .00001; I 2 = 99%), lymphocyte counts (0.15 [0.07–0.23]; P = .0004; I 2 = 100%), basophil counts (0.01 [0.00–0.02]; P = .02; I 2 = 98%), monocyte counts (0.05 [0.02–0.09]; P = .003; I 2 = 99%), and neutrophil-to-lymphocyte ratio (0.24 [0.15–0.33]; P < .00001; I 2 = 98%). There were no significant differences in the eosinophil count (0.02 [−0.01 to 0.05]; P = .19; I 2 = 96%) and monocyte-to-lymphocyte ratio (0.06 [−0.05 to 0.17]; P = .27; I 2 = 100%) between patients with and without MetS, however, the lymphocyte-to-monocyte ratio (0.52 [−0.81 to −0.23]; P = .0005; I 2 = 52%) tended to be significantly lower in patients with MetS. Conclusion: Biomarkers such as total leukocyte count, neutrophil count, lymphocyte count, basophil count, monocyte count and neutrophil-to-lymphocyte ratio are associated with higher levels in patients in MetS and thus can potentially be used for early detection of MetS.

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“…In proliferative diabetic retinopathy and diabetic cardiac fibrosis, the abnormal change of miR-18a-5p expression was associated with the development of complications of diabetes [9,11]. In patients with metabolic syndrome, the miR-18a-5p was lessened relative to control subjects, and its levels correlate with the expression of IL-6 [14]. In this present investigation, the lowly expressed miR-18a-5p restricted the depressed infarct size, Bederson score, and Longa score led by UCA1 loss, which documented that the beneficial impacts of diminished UCA1 on CI/R injury were regulated by miR-18a-5p.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Loss of lncRNA UCA1 ameliorates the injury managed by cerebral ischemia-reperfusion by sponging miR-18a-5p

Yan¹,

Gao²,

Huang³

et al. 2023

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Introduction: Acute ischemic stroke (AIS) is a disease with high morbidity and mortality in the clinic. The current experiments aimed to study the effects of UCA1 interfering miR-18a-5p on cerebral ischemia-reperfusion (CI/R).Material and methods: For rat models undergoing middle cerebral artery infarction (MCAO) surgery, the expression of UCA1 and miR-18a-5p was evaluated by qRT-PCR, and underlying function was identified by detecting infarct size, neurological scores, and inflammation. Luciferase report was applied to verify the relationship between UCA1 and miR-18a-5p. In the cell models, the impacts of UCA1 and miR-18a-5p were validated by CCK-8 assay, flow cytometry analysis, and ELISA. In patients with AIS, Pearson correlation was carried out to unveil the association between UCA1 and miR-18a-5p. Results: The expression of UCA1 was at high levels and miR-18a-5p was at low levels in AIS patients. UCA1 knockdown showed a protective role in infarct size, neurofunction, and inflammation via binding miR-18a-5p. MiR-18a-5p participated in the regulation of UCA1 on cell viability, cell apoptosis, lactate dehydrogenase (LDH) levels, and inflammation. In patients with AIS, overexpression of UCA1 and underexpression of miR-18a-5p had a reverse correlation. Conclusions: Elimination of UCA1 was favourable to the recovery of the rat model and cells from CI/R damage by efficaciously sponging miR-18a-5p.

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PBMCs to Stress-Associated miR-18a-5p and miR-22-3p Ratios as New Indicators of Metabolic Syndrome

Cited by 7 publications

References 24 publications

LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis

LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis

Association of white blood cell parameters with metabolic syndrome: A systematic review and meta-analysis of 168,000 patients

Loss of lncRNA UCA1 ameliorates the injury managed by cerebral ischemia-reperfusion by sponging miR-18a-5p

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