Pcl/Gelatin Nanofibers Incorporated with Starfish Polydeoxyribonucleotides for Potential Wound Healing Applications

Kim, Tae-Hee; Park, Won Sun; Choi, Il‐Whan; Kim, Hyun Woo; Kang, Hyun Wook; Kim, Young‐Mog; Kim, Se-Chang; Jung, Won‐Kyo

doi:10.2139/ssrn.4355347

Cited by 2 publications

(3 citation statements)

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“…It acts as an agonist against the adenosine A 2A receptor and exhibits several biological activities, such as anti‐inflammatory, angiogenesis, antiapoptotic, and tissue repair activities 10 . However, the use of PDRN is limited due to the availability of this extraction source ( O. mykiss and O. keta ) and its current applications, which are mainly used in cosmetics 17 . To overcome these limitations, several studies have investigated the extraction of PDRN from other marine organisms to enhance the cost‐effectiveness compared to PDRN extracted from original extraction sources 10,11,17 …”

Section: Discussionmentioning

confidence: 99%

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Anti‐inflammatory effect of polydeoxyribonucleotides (PDRN) extracted from red alga (Porphyra sp.) (Ps‐PDRN) in RAW 264.7 macrophages stimulated with Escherichia coli lipopolysaccharides: A comparative study with commercial PDRN

Kim,

Heo,

Han

et al. 2023

Cell Biochemistry & Function

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Polydeoxyribonucleotide (PDRN) is a DNA‐derived drug extracted from the sperm cells of Oncorhynchus mykiss or O. keta. PDRN exhibits wound healing and anti‐inflammatory activities by activating adenosine A2A receptor and salvage pathways. However, commercial PDRN products (e.g., Placentex, Rejuvenex, and HiDr) have limitations as they are exclusively extracted O. mykiss and O. keta, which are expensive and can only be used as extraction sources during a specific period when their sperm cells are activated. Therefore, this study aimed to extract PDRN from Porphyra sp. (Ps‐PDRN) and investigate whether it has anti‐inflammatory activity through a comparative study with commercial product. The results indicated that Ps‐PDRN had an anti‐inflammatory effect on Escherichia coli lipopolysaccharides (LPS)‐stimulated RAW 264.7 macrophages. It inhibited nitric oxide production and inducible nitric oxygen synthase protein expression by suppressing phosphorylation of p38 and ERK, without cytotoxicity. Furthermore, Ps‐PDRN promoted cell proliferation and collagen production in human dermal fibroblast. In conclusion, our study confirms that Ps‐PDRN exhibits both anti‐inflammatory and cell proliferative effects. These results indicated that Ps‐PDRN has the potential as a bioactive drug for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

“…The purity of Ps‐PDRN was determined by calculating the absorbance ratio of 260 nm and 280 nm (A260/A280), and the DNA concentration in Ps‐PDRN was determined based on the absorbance at 260 nm using a microplate reader (PowerWave XS2, BioTek Instruments, Inc.) 17 …”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory effect of polydeoxyribonucleotides (PDRN) extracted from red alga (Porphyra sp.) (Ps‐PDRN) in RAW 264.7 macrophages stimulated with Escherichia coli lipopolysaccharides: A comparative study with commercial PDRN

Kim,

Heo,

Han

et al. 2023

Cell Biochemistry & Function

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“…On the other hand, adding gelatin to other biodegradable polymers in making nano bers can improve the mechanical properties, homogeneous morphology, and diameter of nano bers 21 . New methods using ultrathin multi-component nano bers have been presented for the rapid development of biocompatible nanodrug delivery systems, good drug entrapment e ciency, controlled release, local delivery, and clinically easy 22,23 . Immobilizing NPs onto a surface to increase the effective surface area is one method to improve the interaction between the nano bers' surface and the cultured cells 24 .…”

Section: Introductionmentioning

confidence: 99%

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Esmaeilneia,

Dehkharghani,

Benisi

2023

Preprint

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In this research, genistin was obtained by extracting from soybean meal powder, and then by adding polycaprolactone (PCL), genistin nanocapsules were prepared. For the first time, we used a lipophilic nanophase (encapsulated genistin) coated in hydrophilic nanofibers (gelatin /polyvinyl alcohol) as a dual nanosystem by the electrospinning method. In the approach, the nanofibers mimic the natural extracellular matrix, interact favorably with cells being cultured from one side, and raise the local concentration of a bioactive compound at the cell surface. The encapsulated drug was loaded in fibers with various percentages, which showed appropriate and significant controlled release using high-performance liquid chromatography (HPLC). To prove the experiments, analysis using an ultraviolet-visible spectrometer (UV-Vis), 1 H NMR spectrometer, Fourier transforms infrared spectrometer (FTIR), mechanical test, scanning electron microscope (SEM) and microscope transmission electron microscopy (TEM) was performed. Finally, excellent biological effects, viability, and non-toxicity of the sample synthesized by the MTT method and significant proliferation and adhesion on mouse fibroblast cell line L929 were obtained.

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Pcl/Gelatin Nanofibers Incorporated with Starfish Polydeoxyribonucleotides for Potential Wound Healing Applications

Cited by 2 publications

References 0 publications

Anti‐inflammatory effect of polydeoxyribonucleotides (PDRN) extracted from red alga (Porphyra sp.) (Ps‐PDRN) in RAW 264.7 macrophages stimulated with Escherichia coli lipopolysaccharides: A comparative study with commercial PDRN

Anti‐inflammatory effect of polydeoxyribonucleotides (PDRN) extracted from red alga (Porphyra sp.) (Ps‐PDRN) in RAW 264.7 macrophages stimulated with Escherichia coli lipopolysaccharides: A comparative study with commercial PDRN

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

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