PCN-224/nano-zinc oxide nanocomposite-based electrochemiluminescence biosensor for HPV-16 detection by multiple cycling amplification and hybridization chain reaction

Wu, Di; Dong, Wenshuai; Yin, Tengyue; Jie, Guifen; Zhou, Hong

doi:10.1016/j.snb.2022.132659

Cited by 20 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The linear response range, LOD, and sensitivity of Au|c-DNA|t-DNA|p-DNA|DHLA-AgNCs for determining the HPV gene are comparable to, or even better than, some previously reported biosensors (Table S1). − Reproducibility of the low-triggering-potential and narrow-potential-window ECL sensors is measured at various t-DNA concentrations by repeating three times. The relative standard deviation (RSD) of the maximum ECL intensity of Au|c-DNA|t-DNA|p-DNA|DHLA-AgNCs toward the t-DNA sample with the same concentration is lower than 5% (inset of Figure A), which clearly demonstrates the feasibility and reproducibility of the narrow triggering-potential-window ECL gene assay strategy with DHLA-AgNCs as a label.…”

Section: Resultsmentioning

confidence: 99%

Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay

Ai,

Gao,

Ren

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

A low-triggering potential and a narrow-potential window are anticipated to decrease the electrochemical interference and cross talk of electrochemiluminescence (ECL). Herein, by exploiting the low oxidative potential (0.82 V vs Ag/AgCl) of dihydrolipoic acid-capped sliver nanoclusters (DHLA-AgNCs), a coreactant ECL system of DHLA-AgNCs/hydrazine (N2H4) is proposed to achieve efficient and oxidative-reduction ECL with a low-triggering potential of 0.82 V (vs Ag/AgCl) and a narrow-potential window of 0.22 V. The low-triggering-potential and narrow-potential-window nature of ECL can be primarily preserved upon labeling DHLA-AgNCs to probe DNA and immobilizing DHLA-AgNCs onto the Au surface via sandwiched hybridization, which eventually enables a selective ECL strategy for the gene assay at +0.82 V. This gene assay strategy can sensitively determine the gene of human papillomavirus from 10 to 1000 pM with a low limit of detection of 5 pM (S/N = 3) and would open a way to improve the applied ECL bioassay.

show abstract

Section: Resultsmentioning

confidence: 99%

Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay

Ai,

Gao,

Ren

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Next, S1 (5 μL, 0.5 μM) was dripped onto the AuNPs surface and incubated for the night at 4 °C. After that, 6-mercapto-1hexanol (MCH) (5 μL, 0.5 mM) blocked the nonspecific binding sites for 1 h, and DA-S2 (5 μL, 0.5 μM) was added on the electrode surface and incubated for 1 h. 44 Afterward, 5 μL of various concentrations of HBV and 2 μL of Exo III (20 U/ μL) were successively added on the electrode surface and reacted at 37 °C for 100 min. The electrode surface was rinsed with phosphate buffer saline (PBS) after each step above.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

High-Efficiency Aluminum–Metal Organic Framework/HEPES Electrochemiluminescence System for Ultrasensitive Detection of HBV DNA

et al. 2023

View full text Add to dashboard Cite

In this work, a novel aluminum metal–organic framework (Al-MOF)/N-2-hydroxyethylpiperazine-N′-ethane-sulfonic acid (HEPES) system with an excellent electrochemiluminescence (ECL) property was developed. First, Al-MOF was successfully synthesized through a one-pot solvothermal method by using 9,10-di(p-carboxyphenyl)anthracene (DPA) as the organic luminescence ligand and Al3+ as the metal node. Compared with DPA, Al-MOF showed high ECL intensity and excellent stability without an additional coreactant in the HEPES buffer. The corresponding ECL mechanism was studied in detail, verifying HEPES was not only the buffer in the system but also the coreactant of Al-MOF. In particular, the system of Al-MOF/HEPES showed a high ECL efficiency of 30.0%, taking the Ru(bpy)3 2+ system as the standard. In addition, the ECL signal of Al-MOF was effectively quenched by dopamine (DA). The biosensor for HBV DNA detection was constructed through the ECL signal on–off–on mode of DNA specific recognition integrated with the DNA walker signal amplification strategy. The ultrasensitive detection for HBV DNA was achieved with a linear range of 100 aM to 10 pM and a limit of detection (LOD) of 62.1 aM. This work proposed a high-efficiency Al-MOF/HEPES system, providing a new viewpoint for a coreactant-free system in the field of ECL.

show abstract

“…21 The absorption peaks at 965, 1544, and 1604 cm −1 were associated with the C−H oscillation of the pyrrole ring and the C�C stretching functional group of the benzene ring, respectively. 29 Simultaneously, the strong absorption peaks at 3432 and 656 cm −1 were the result of O−H and Zr− OH stretching vibrations, 31 respectively, which also indicated the successful synthesis of PCN-224. TGA was performed under a N 2 atmosphere to assess the thermal stability of PCN-224.…”

Section: Characterization Of Pcn-224 and Agnps@pcn-224mentioning

confidence: 99%

Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Wang,

Pan,

Wang

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Organophosphorus pesticides (OPPs) are extensively used in agricultural production, and the contamination caused by their residues has raised significant concerns regarding potential threats to human health. Herein, a novel fluorescence nanoprobe based on an enzyme-mediated silver nanoparticle-modified metal organic framework (AgNPs@PCN-224) was successfully prepared for the rapid detection of OPPs. Initially, AgNPs@PCN-224 were synthesized by reducing silver nitrate (AgNO3) using sodium borohydride (NaBH4) embedded into luminescent PCN-224. This triggered the inner filter effect, leading to fluorescence quenching. Meanwhile, under the catalysis of acetylcholinesterase (AChE) and choline oxidase (CHO), acetylcholine (ATCh) was decomposed to hydrogen peroxide (H2O2), which could destroy AgNPs to form Ag+ released from PCN-224 for fluorescence recovery. Instead, fenitrothion, an OPP, inhibited AChE activity, allowing the quenched fluorescence to be reactivated. Under the current optimum conditions, the fluorescence intensity had a good correlation (Y = −728.5370X + 2178.4248, R 2 = 0.9869) over a dynamic range of fenitrothion concentrations from 0.1 to 500 ng/mL, with an LOD of 0.037 ng/mL. In addition, the anti-interference ability and robustness of the proposed sensor was verified for the monitoring of fenitrothion in tea with recoveries of 87.67–103.72% and the relative standard deviations (RSD) < 5.43%, indicating that the system has excellent prospects for OPP determination in practical applications. Furthermore, this work provides a universal platform for screening other enzyme inhibitors to detect OPPs.

show abstract

PCN-224/nano-zinc oxide nanocomposite-based electrochemiluminescence biosensor for HPV-16 detection by multiple cycling amplification and hybridization chain reaction

Cited by 20 publications

References 44 publications

Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay

Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay

High-Efficiency Aluminum–Metal Organic Framework/HEPES Electrochemiluminescence System for Ultrasensitive Detection of HBV DNA

Ultrasensitive Fluorescence Platform Based on AgNPs In Situ-Incorporated Zr-MOFs for the Detection of Organophosphorus Pesticides

Contact Info

Product

Resources

About