PCOS and Role of Cumulus Gene Expression in Assessing Oocytes Quality

Sayutti, Nurainie; Abu, Muhammad Azrai; Ahmad, Mohd Faizal

doi:10.3389/fendo.2022.843867

Cited by 30 publications

(24 citation statements)

References 85 publications

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“…Complex biological processes, such as those involved in human reproduction, require different genes working in an orchestrated way to ensure regulatory networks ( de la Fuente, 2010 ). Recent studies of global gene expression in oocytes and sperm have demonstrated important target genes involved in conditions affecting human reproduction ( Hermann et al, 2018 ; Li et al, 2021 ; Ntostis et al, 2021 ; Santiago et al, 2021 ; Sayutti et al, 2022 ). In the present study, prior filtering of meiosis-related genes was performed, and these genes had their differential gene expression evaluated in female and male gonads and gametes under physiological and pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Expression profiles of meiotic genes in male vs. female gonads and gametes: Insights into fertility issues

et al. 2023

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Gametes are specialized cells that, at fertilization, give rise to a totipotent zygote capable of generating an entire organism. Female and male germ cells undergo meiosis to produce mature gametes; however, sex-specific events of oogenesis and spermatogenesis contribute to specific roles of gametes in reproductive issues. We investigate the differential gene expression (DGE) of meiosis-related genes in human female and male gonads and gametes in normal and pathological conditions. The transcriptome data for the DGE analysis was obtained through the Gene Expression Omnibus repository, comprising human ovary and testicle samples of the prenatal period and adulthood, additionally to male (non-obstructive azoospermia (NOA) and teratozoospermia), and female (polycystic ovary syndrome (PCOS) and advanced maternal age) reproductive conditions. Gene ontology terms related to meiosis were associated with 678 genes, of which 17 genes in common were differentially expressed between the testicle and ovary during the prenatal period and adulthood. Except for SERPINA5 and SOX9, the 17 meiosis-related genes were downregulated in the testicle during the prenatal period and upregulated in adulthood compared to the ovary. No differences were observed in the oocytes of PCOS patients; however, meiosis-related genes were differentially expressed according to the patient’s age and maturity of the oocyte. In NOA and teratozoospermia, 145 meiosis-related genes were differentially expressed in comparison to the control, including OOEP; despite no recognized role in male reproduction, OOEP was co-expressed with genes related to male fertility. Taking together, these results shed light on potential genes that might be relevant to comprehend human fertility disorders.

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Section: Discussionmentioning

confidence: 99%

Expression profiles of meiotic genes in male vs. female gonads and gametes: Insights into fertility issues

et al. 2023

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“…Of note, a good balance of cumulus growth and apoptosis is required for optimal ovulation. Cumulus cell apoptosis causes poor oocyte results, embryo fragmentation, and impaired blastocyst development [59,61]. These results suggest that the low concentration of fructose would produce a good quality COCs that the higher ones due to the effects of the higher concentration of fructose alters the expression of apoptotic genes and HIF-1α.Other studies proved that women who were successful in becoming pregnant had a considerably lower percentage of apoptotic cells than those who were unsuccessful [62].…”

Section: Effect Of Adding Different Concentrations Of Fructose To Ivm...mentioning

confidence: 96%

“…In women, the small dosage supplementation of fructose to the in-vitro maturation media suggests positive expression of HAS2, PTX3, GREM1, and VCAN that correlated with good quality oocytes and can be used as an indicator among PCOS women. These genes are responsible for cumulus expansion, cumulus cell apoptosis, and glucose metabolism [59]. The higher dose of fructose was reported to reproduce some of the features of metabolic syndrome, most changes were caused by oxidative stress and insulin resistance [54].…”

Section: Effect Of Adding Different Concentrations Of Fructose To Ivm...mentioning

confidence: 99%

“…The regulation of the apoptotic genes resulted in the normal expansion of COCs and production of good quality embryos. The increased fold changes for the apoptotic genes and may HIF-1α indicate a bad quality of oocytes and hence decreased the yield of embryos after in vitro maturation [59,60]. Of note, a good balance of cumulus growth and apoptosis is required for optimal ovulation.…”

Section: Effect Of Adding Different Concentrations Of Fructose To Ivm...mentioning

confidence: 99%

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The Effects of Fructose on the Developmental Competence, Biochemical Biomarkers and Apoptotic Gene Expression by Murine Oocytes

Helmy

Halawa²,

Montaser

et al. 2023

Egyptian Journal of Veterinary Sciences

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T HE goal of the current study was to investigate the effects of fructose supplementation in the culture medium on in vitro maturation, and the developmental competence of murine oocytes, biochemical markers and the expression of apoptotic and HIF-1α in cumulus oocyte cells (COCs). Murine oocytes were matured in IVF media supplemented with 1.25, 2.75 and 5-mM fructose. The developmental parameters (MI, MII, GVBD, degenerated and activated cells) were examined with NO, GPX, GSH, MDA, and TAC biomarkers. The expression of mRNA for apoptotic genes by COCs were evaluated. The low dosage improves significantly (P<0.05) the developmental competence markers than the control and higher doses of fructose. In vitro-matured mouse oocytes with 1.25 mM fructose showed a significant increase (P<0.05) in NO level in the In-vitro matured when compared with the control and other groups. The GPX concentrations in the in-vitro matured oocytes showed a significant decrease (P<0.05) with 5mM fructose. The MDA levels in the vitro-matured oocytes were higher (P<0.05) when the murine oocytes were exposed to 2.75 mM of fructose. The TAC in the oocyte showed no changes (P˂0.05) when the oocytes were exposed to both 1.25 mM. Moreover, the supplementation of IVM media showed a down regulation of apoptotic and HIF-1α genes when compared with the control and high dosage supplementation (P<0.05). Altogether, the supplementation of IVM media with lower dosage of fructose improves the developmental competence markers in the murine oocytes via its effects on oxidant and antioxidant activity and downregulation of apoptotic and HIF-1α genes.

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“…This syndrome produces side effects related to women's overall health, such as abnormal hair growth, weight gain, irregular menstrual cycle, infertility and depression [2,3]. Almost 70% of women with PCOS suffer from infertility [4,5], and this condition is one of the common reasons for female infertility [6,7]. PCOS occurs in women during their reproductive years [8][9][10], between 18 and 45 years old.…”

Section: Introductionmentioning

confidence: 99%

Performance Analysis of a Novel Hybrid Segmentation Method for Polycystic Ovarian Syndrome Monitoring

et al. 2023

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Experts have used ultrasound imaging to manually determine follicle count and perform measurements, especially in cases of polycystic ovary syndrome (PCOS). However, due to the laborious and error-prone process of manual diagnosis, researchers have explored and developed medical image processing techniques to help with diagnosing and monitoring PCOS. This study proposes a combination of Otsu’s thresholding with the Chan–Vese method to segment and identify follicles in the ovary with reference to ultrasound images marked by a medical practitioner. Otsu’s thresholding highlights the pixel intensities of the image and creates a binary mask for use with the Chan–Vese method to define the boundary of the follicles. The acquired results were compared between the classical Chan–Vese method and the proposed method. The performances of the methods were evaluated in terms of accuracy, Dice score, Jaccard index and sensitivity. In overall segmentation evaluation, the proposed method showed superior results compared to the classical Chan–Vese method. Among the calculated evaluation metrics, the sensitivity of the proposed method was superior, with an average of 0.74 ± 0.12. Meanwhile, the average sensitivity for the classical Chan–Vese method was 0.54 ± 0.14, which is 20.03% lower than the sensitivity of the proposed method. Moreover, the proposed method showed significantly improved Dice score (p = 0.011), Jaccard index (p = 0.008) and sensitivity (p = 0.0001). This study showed that the combination of Otsu’s thresholding and the Chan–Vese method enhanced the segmentation of ultrasound images.

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PCOS and Role of Cumulus Gene Expression in Assessing Oocytes Quality

Cited by 30 publications

References 85 publications

Expression profiles of meiotic genes in male vs. female gonads and gametes: Insights into fertility issues

Expression profiles of meiotic genes in male vs. female gonads and gametes: Insights into fertility issues

The Effects of Fructose on the Developmental Competence, Biochemical Biomarkers and Apoptotic Gene Expression by Murine Oocytes

Performance Analysis of a Novel Hybrid Segmentation Method for Polycystic Ovarian Syndrome Monitoring

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