PCP and Septins Govern the Polarized Organization and Mechanics of the Actin Cytoskeleton During Convergent Extension

Devitt, Caitlin C.; Weng, Shinuo; Bejar-Padilla, Vidal  D.; Alvarado, José; Wallingford, John B.

doi:10.2139/ssrn.4130564

Cited by 2 publications

(2 citation statements)

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“…Another potential target of ippk-1 /IP6 is the septin family of cytoskeletal components. Work in Xenopus has shown that septins play important roles in the distribution of actin networks during collective cell behaviors, including convergent extension (Devitt et al, 2024). Septin proteins co-localize and function alongside PCP components, including VANG2 and PRKL2.…”

Section: Discussionmentioning

confidence: 99%

IPPK-1 and IP6 contribute to ventral nerve cord assembly inC. elegans

Noblett,

Roenspies,

Flibotte

et al. 2024

Preprint

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Inositol phosphates (IPs) play important roles in nervous system development and function. One of these roles uncovered by loss-of-function studies, is that IP isomers are essential for proper neural tube formation. In this study, we show that inositol pentakisphosphate 2-kinase (IPPK-1), the kinase that phosphorylates IP5 to generate IP6, is involved in assembling the ventral nerve cord (VNC) in C. elegans. We show that mutations in ippk-1 lead to the mispositioning of motor neurons along the VNC of newly hatched larvae. These positioning defects reflect disruption of VNC assembly during embryogenesis, as ippk-1 embryos display improper alignment of VNC neuroblasts and delays in rosette-mediated convergent extension (CE). We further show that injection of exogenous IP6 into the gonads of ippk-1 mutants can rescue both embryonic and neuron positioning defects. Our findings indicate that inositol metabolism is important for regulating CE in C. elegans and suggest that IP isomers play a conserved role in the formation of central nerve cords.

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Section: Discussionmentioning

confidence: 99%

IPPK-1 and IP6 contribute to ventral nerve cord assembly inC. elegans

Noblett,

Roenspies,

Flibotte

et al. 2024

Preprint

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show abstract

“…We assessed the role of Pk2 in AJ remodeling and tissue fluidity in Xenopus neuroectoderm (NE), a "fluid-like" epithelium characterized by intensive AJ remodeling, cell intercalation and apical domain variability (Baldwin et al, 2022a;Christodoulou and Skourides, 2022;Matsuda et al, 2023;Williams et al, 2014). A previously characterized splicing-blocking morpholino (MO) (Butler and Wallingford, 2015;Butler and Wallingford, 2018;Devitt et al, 2024;Shindo et al, 2019) was co-injected with a lineage tracer into one dorsal blastomere at the 4-to 8-cell stage, to unilaterally deplete Pk2 in the NE (Fig. 1A).…”

Section: Pk2 Depletion Decreases the Rate Of Aj Remodeling In The Neu...mentioning

confidence: 99%

Prickle2 regulates apical junction remodeling and tissue fluidity during vertebrate neurulation

Matsuda,

Sokol

2024

Preprint

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The process of folding the flat neuroectoderm into an elongated neural tube depends on tissue fluidity, a property that allows epithelial deformation while preserving tissue integrity. Neural tube folding also requires the planar cell polarity (PCP) pathway. Here, we report that Prickle2 (Pk2), a core PCP component, increases tissue fluidity by promoting the remodeling of apical junctions (AJs) in Xenopus embryos. This Pk2 activity is mediated by the unique evolutionarily conserved Ser-Thr-rich region (STR) in the carboxyterminal half of the protein. Mechanistically, the effects of Pk2 require Rac1 and are accompanied by increased cadherin dynamics and destabilization of tricellular junctions, the hotspots of AJ remodeling. Notably, Pk2 depletion leads to the accumulation of mediolaterally oriented cells in the neuroectoderm, whereas the overexpression of Pk2 or Pk1 containing the Pk2-derived STR promotes cell elongation along the anteroposterior axis. We propose that Pk2-dependent regulation of tissue fluidity contributes to anteroposterior tissue elongation in response to extrinsic cues.

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PCP and Septins Govern the Polarized Organization and Mechanics of the Actin Cytoskeleton During Convergent Extension

Cited by 2 publications

References 0 publications

IPPK-1 and IP6 contribute to ventral nerve cord assembly inC. elegans

IPPK-1 and IP6 contribute to ventral nerve cord assembly inC. elegans

Prickle2 regulates apical junction remodeling and tissue fluidity during vertebrate neurulation

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