2023
DOI: 10.1007/978-1-0716-3024-2_18
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PCR Allele Competitive Extension (PACE)

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Cited by 5 publications
(3 citation statements)
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“…If genome elimination of the maternal chromosomes occurred, the progeny would only contain the wild-type allele from the wild-type parent. We therefore used either PCR Allele Competitive Extension (PACE™) ( von Maydell, 2023 ) or Sanger sequencing to genotype this region of CENH3 in progeny ( Figure 2 ). The pollen donors in these crosses were homozygous for the wild-type CENH3 sequence.…”
Section: Resultsmentioning
confidence: 99%
“…If genome elimination of the maternal chromosomes occurred, the progeny would only contain the wild-type allele from the wild-type parent. We therefore used either PCR Allele Competitive Extension (PACE™) ( von Maydell, 2023 ) or Sanger sequencing to genotype this region of CENH3 in progeny ( Figure 2 ). The pollen donors in these crosses were homozygous for the wild-type CENH3 sequence.…”
Section: Resultsmentioning
confidence: 99%
“…The PCR Allele Competitive Extension (PACE) genotyping method, described in von Maydell (2023), is a recently developed method for qPCR-based SNP genotyping [21]. Though recently developed, this method has been applied in agricultural sciences before [22,23].…”
Section: Pace Methodsmentioning
confidence: 99%
“…Two allele-specific forward primers with different fluorescent signals (also HEX and FAM) and one common reverse primer are used to target an SNP of interest. An advantage of the PACE method is that SNPs can be determined to be either homozygous or heterozygous; if one fluorescent signal is produced, the SNP is homozygous, and if both signals are produced then the SNP is heterozygous [21]. The main difference between this method and the KASP method is the type of reagent used for PCR.…”
Section: Pace Methodsmentioning
confidence: 99%