PCR amplification of 18S rRNA, single cell protein production and fatty acid evaluation of some naturally isolated microalgae

Rasoul‐Amini, Sara; Ghasemi, Younes; Morowvat, Mohammad Hossein; Mohagheghzadeh, Abdolali

doi:10.1016/j.foodchem.2009.02.025

Cited by 60 publications

(30 citation statements)

References 16 publications

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“…The results of this study are in agreement with the presented results by other researchers performed in fatty acid profiling of D. salina. 33 As the results show, we detected some significant FAs/FAMEs with various pharmaceutical and nutritional applications. The observed FAs/FAMEs profile confirms the potential application of the identified strain for biodiesel production.…”

Section: Fatty Acid Composition Analysismentioning

confidence: 64%

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

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Objective: Microalgal pharmaceutical biotechnology is mainly dependent on the biomass yield and also the final concentration of the obtained lipids. β-carotene is one of the most precious nutraceuticals, of both preventive and therapeutics importance in pharmacy and medicine. Dunaliella salina is known as famous β-carotene producer which could accumulate the β-carotene up to 10% of its dry cell weights. The amount of different macro and micronutrients in D. salina culture medium defines its productivity and β-carotene content. Methods: In this study, the effects of sulfur, iron and manganese deprivation, on cell growth and β-carotene biosynthesis in a naturally isolated strain of D. salina was examined. Besides, the fatty acid profile of the naturally isolated strain was also investigated. Results: Sulfur, iron and manganese deprivation caused a noticeable decrease in the cell growth of D. salina. On the other hand, in nutrient depleted media, the maximum β-carotene concentration was significantly improved (14.616 mg L-1 in sulfur starvation, 14.994 mg L-1 in iron starvation and 10.119 mg L-1 in manganese starvation media) compared with initial values (6.753 mg L-1) in basic culture medium. The obtained fatty acids from the studied microalgal strain found to be some important saturated, monounsaturated and polyunsaturated fatty acids. Conclusion: Owing to its significant growth rate, β-carotene contents and fatty acid profile; the naturally isolated microalgal strain could be exploited as a potential producer strain. Besides, the nutrient limitation strategy could be effectively employed to improve the β-carotene production procedure in D. salina. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Section: Fatty Acid Composition Analysismentioning

confidence: 64%

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Shaker¹,

Morowvat²,

Ghasemi³

2017

JYP

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“…Therefore, a biochemical approach has gained an interest for the characterization of the frankial strains on the basis of fatty acid composition in the last few decades [3, 31 -33]. It is true that pharmaceutical importance of the fatty acid is increasing day by day [16,17]. In addition to this, it has also been proved useful for the chemotaxonomic studies of many environmentally relevant and biotechnologically important species at the family, genus and species level [31 -36].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Frankia strains nodulating HippÖphae salicifolia D. Don using FAME profiling as Chemotaxonomic markers

Mishra

Singh

2010

J. Basic Microbiol.

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Twelve Frankia strains isolated from Hippophae salicifolia D. Don or Alnus glutinosa or Comptonia peregrine, showed the significant variation in fatty acid composition viz. palmitic acid (16:0), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3), arachidic acid (20:0) and erucic acid (22:1) suggesting the strain specific variability among the Frankia strains. Presence of Erucic acid (22:1), a major component of the oil obtained from the seeds of Brassica sp., albeit in lesser amount in the few studied frankial strains, is the first report. Cluster analysis on the basis of fatty acid composition suggests the presence of two distinct clusters with similitude coefficient ranging from 0.75 to 1.00. Cluster I with HsIi2 showed great divergence from other 11 frankial strains (Cluster II). The two sub groups were distinguished in cluster II: IIa contained five strains isolated from H. salicifolia and these strains are distantly related to the strains of cluster IIb isolated from different host. There is high degree of similarity among the frankial strains of Cluster IIb which suggests that the frankial strains might be evolved from the same ancestor. FAME profiling might be useful tool in the study of polyphasic approach based taxonomy and phylogenetic relationship.

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“…Genomic DNA of microalgal strains was prepared according to Rasoul-Amini et al (2009). DNA fragments of ∼800 and ∼700 bp were amplified from genomic DNA of microalgal strains with polymerase chain reaction (PCR) by using universal primers against the 16S/18S rRNA genes, respectively.…”

Section: Methodsmentioning

confidence: 99%

Biotransformation of monoterpenes by immobilized microalgae

2010

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This paper reports the biotransformation of carvone, limonene, β-pinene, thymol, and linalool using whole-cell-immobilized microalgal strains isolated from paddy fields of Iran. The strains was recognized by morphological characterization and assigned according to amplified 16S/18S rRNA genes by PCR. Ten unialgal strains including Chlorella, Oocystis, Chlamydomonas, and Synechococcus were immobilized in calcium alginate beads. After a 24-h incubation with substrates, characterization and identification of biotransformation products were done by GC/MS. None of the isolated immobilized microalgae converted β-pinene. In contrast, most of these strains biotransformed carvone and limonene to the related compounds. Some strains only reduced the C=C double bond to yield the dihydrocarvone isomers while others reduced the ketone to give the dihydrocarveol. The transformation ratio showed that Oocystis sp. MCCS 033 and Synechococcus sp. MCCS 035 produced dihydrocarvone isomers with the highest efficiency. Furthermore, limonene was converted into a mixture of five corresponding products and the maximum yield was 52.1% for carvone, the bioconverted product. Only one strain, Synechococcus sp. MCCS 034, oxidized thymol, and the product obtained from thymol was thymoquinone. Also, linalooloxide isomers and dihydrolinalool were obtained from linalool, and finally dihydrolinalool was the main product. These results showed a novel conversion pathway of linalool-forming dihydrolinalool.

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PCR amplification of 18S rRNA, single cell protein production and fatty acid evaluation of some naturally isolated microalgae

Cited by 60 publications

References 16 publications

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina

Diversity of Frankia strains nodulating HippÖphae salicifolia D. Don using FAME profiling as Chemotaxonomic markers

Biotransformation of monoterpenes by immobilized microalgae

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