PCR amplification of mini-exon genes differentiates Trypanosoma cruzi from Trypanosoma rangeli

Murthy, Vimal K.; Dibbern, Kurt; Campbell, David A.

doi:10.1016/0890-8508(92)90022-p

Cited by 86 publications

(53 citation statements)

References 18 publications

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“…Certainly, the close relationship between T. rangeli and T. cruzi evident from ssu rRNA analysis has also been confirmed by comparison of miniexon sequences (Stevens & Gibson, unpublished data). The miniexon sequence also confirms that the T. rangeli isolate used in the current study is a bona fide T. rangeli (Murthy et al 1992). …”

Section: Discussionsupporting

confidence: 56%

“…In the current study T. rangeli, albeit only a single isolate (RGB -Basel), is classified firmly in a clade with a range of Schizotrypanum species (bootstrap 97%); the classification of this isolate as T. rangeli is supported by preliminary results from analysis of the miniexon which indicate it to be of the correct size and sequence according to Murthy et al (1992). T. rangeli and T. cruzi also cluster together (bootstrap >90%) and separate from Salivarian trypanosomes in phylogenetic analyses of miniexon sequences (Stevens & Gibson, unpublished data).…”

Section: Phylogenetic Analysis the Phylogram (supporting

confidence: 53%

See 1 more Smart Citation

The Evolution of Trypanosomes Infecting Humans and Primates

Stevens

Noyes

Gibson³

1998

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 56%

Section: Phylogenetic Analysis the Phylogram (supporting

confidence: 53%

The Evolution of Trypanosomes Infecting Humans and Primates

Stevens

Noyes

Gibson³

1998

Mem. Inst. Oswaldo Cruz

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“…However, current PCR assays used for mixed infection detection show some disadvantages such as the amplification of bands of similar size both in T. cruzi and T. rangeli 27,28 , the amplification of polymorphic fragments 11,19 , and bias to T. cruzi in the case of mixed T. cruzi and T. rangeli infection 9,33,36 . Therefore, it is necessary to develop new techniques as better options to specifically identify each of these parasite species in mixed infections.…”

Section: Introductionmentioning

confidence: 99%

“…Due to direct microscopic detection of trypanosomes, the traditional method for assessment of infection in vectors is not able to distinguish T. cruzi from T. rangeli infection, several polymerase chain reaction techniques have been developed 3,4,5,6,9,13,19,28,33,36,40,41 . However, current PCR assays used for mixed infection detection show some disadvantages such as the amplification of bands of similar size both in T. cruzi and T. rangeli 27,28 , the amplification of polymorphic fragments 11,19 , and bias to T. cruzi in the case of mixed T. cruzi and T. rangeli infection 9,33,36 .…”

Section: Introductionmentioning

confidence: 99%

Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes

Pavía

Vallejo

Montilla

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYTrypanosoma rangeli is non pathogenic for humans but of important medical and epidemiological interest because it shares vertebrate hosts, insect vectors, reservoirs and geographic areas with T. cruzi, the etiological agent of Chagas disease. Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively. Both PCRs were able to appropriately detect all T. cruzi or T. rangeli experimentally infected-triatomines, as well as the S35/S36 PCR which amplifies the variable region of minicircle kDNA of T. cruzi. In mixed infections, whereas T. cruzi DNA was amplified in 100% of samples with TcH2AF/R and S35/S36 PCRs, T. rangeli was detected in 71% with TrF/R2 and in 6% with S35/S36. In a group of Rhodnius colombiensis collected from Coyaima (Colombia), T. cruzi was identified in 100% with both PCRs and T. rangeli in 14% with TrF/R2 and 10% with S35/S36 PCR. These results show that TcH2AF/R and TrF/R2 PCRs which are capable of recognizing all T. cruzi and T. rangeli strains and lineages could be useful for diagnosis as well as for epidemiological field studies of T. cruzi and T. rangeli vector infections.

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“…8 In addition, other sets of primers have been reported to give PCR products that are different in size for T. cruzi and T. rangeli when the parasites are present in separate PCRs. 9,10 However, these primers have not been tested in mixed infections.…”

mentioning

confidence: 99%

Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors.

Dorn

Engelke²,

Rodas³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. For effective control programs, accurate assessment of Trypanosoma cruzi infection in vectors is essential and has traditionally been performed by microscopic examination. For particular vectors and not others, polymerase chain reaction (PCR) analysis of fecal samples recently has been shown to be an effective means of detection. The sensitivities of the PCR and microscopy for detection of T. cruzi in different anatomic sites were compared in the two major vectors of Guatemala, Triatoma dimidiata and Rhodnius prolixus. Preliminary studies established that T. cruzi can be detected by the PCR in the presence of 90% T. rangeli. One hundred thirty-five vectors were collected, and samples were obtained from the rectum, intestines, and stomach and analyzed by microscopy and the PCR. For Triatoma dimidiata rectal samples, the PCR sensitivity (39.1% T. cruzi positive) and the microscopic sensitivity (24.6% positive) was not significantly different. However, in R. prolixus, the PCR proved significantly more sensitive than microscopy: 57.6% positive by PCR compared with 22.7% by microscopy. Rectal samples showed the highest rates of infection followed by intestine and stomach samples. However, 10

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PCR amplification of mini-exon genes differentiates Trypanosoma cruzi from Trypanosoma rangeli

Cited by 86 publications

References 18 publications

The Evolution of Trypanosomes Infecting Humans and Primates

The Evolution of Trypanosomes Infecting Humans and Primates

Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes

Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors.

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