PCR Amplification of the IS 6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Evaluation of Molecular Tools for Detection and Drug Susceptibility Testing of Mycobacterium tuberculosis in Stool Specimens from Patients with Pulmonary Tuberculosis

“…This may have been because of differential DNA extraction efficiency or possibly because of differential DNA contamination with PCR inhibitors, a subject that should be investigated in future research. In some regions, strains of M. tuberculosis that lack the IS6110 gene have been reported, and in these locations, alternative M. tuberculosisspecific primers may require evaluation (3).…”

Fecal polymerase chain reaction for Mycobacterium tuberculosis IS6110 to distinguish Crohn’s disease from intestinal tuberculosis

“…For ITB, these included any one of the following: [16,17] (1) mucosal and/or surgical biopsies with caseating granulomas; (2) intestinal granulomatous inflammation accompanied by extra-intestinal confirmed tuberculosis with caseating granulomatous inflammation and/or acid fast bacilli; (3) endoscopic evidence of ileocecal ulceration, nodularity and/or stenosis, with non-caseating granulomas on biopsy and complete endoscopic resolution after a 9-month course of anti-tuberculous therapy; (4) culture of M. tuberculosis from mucosal or surgical biopsy of the intestine. Diagnostic criteria for CD included all the following: [18] (1) bowel symptoms and characteristic endoscopic and/or radiographic features of CD, including small and/or large bowel involvement, and skip lesions (at least two discontinuous discrete segments of ulceration or pseudopolyp formation with intervening normal mucosa); [19] (2) mucosal biopsy showing non-caseating granulomas of typical morphology as described earlier [9], or resection specimen showing transmural inflammation or non-caseating granulomas; (3) clinical and biochemical response to therapy with mesalazine, corticosteroids or immunosuppressive drugs over a two year period of follow up; (4) absence of AFB by histology or culture; and (5) absence of pulmonary infiltrates on chest radiographs.…”

“…We have shown that fecal PCR, targeting the IS6110 sequence of the M. tuberculosis genome, can be useful in diagnosing ITB and active pulmonary tuberculosis where swallowed sputum contributes to mycobacterial DNA in the stool [16]. We undertook the current study to determine whether fecal TB PCR would discriminate between ITB and CD in India.…”

What is the most accurate method for the diagnosis of intestinal tuberculosis?

“…In a relatively larger case series including 60 patients with intestinal tuberculosis and 20 patients with Crohn's disease, the PCR specifity was 95%, while the sensitivity remained up to 21.6% (19). Ramadass et al demonstrated fecal PCR sensitivity and specifity as 88% and 100%, respectively (20). In a series of 20 patients with intestinal tuberculosis and 20 patients with Crohn's disease, PCR sensitivity was 30%, and specifity was 95%, and the sensitivity and specifity for demonstrations of ARB and caseification necrosis were 45% and 100%, respectively, in the diagnosis of intestinal tuberculosis (21).…”