PCR analysis of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola andFusobacterium nucleatum in middle ear effusion

Külekçi, Güven; Çıftçı, Sevgi; Kılıç, Abdullah; Türkoğlu, Salih; Badur, Selim; Develioğlu, Ömer Necati; Leblebicioğlu, Binnaz; Külekçi, Mehmet

doi:10.1006/anae.2001.0400

Cited by 9 publications

(11 citation statements)

References 31 publications

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“…As our study population was similar to that study, we thought it was important to detect T. denticola which was also previously detected in MEE samples 8. In our current study, T. denticola was detected from only four (20%) saliva samples and one (5%) nasopharyngeal secretion sample, while it was not detected in any middle ear effusion sample.…”

Section: Discussionsupporting

confidence: 83%

“…In our previous study, bacterial DNA were determined from 42 % of the middle ear effusion (MEE) samples, which, 37 % of them were positive for Fusobacterium nucleatum and one of them was positive for both F. nucleatum and Treponema denticola 8. T. denticola was found to be the second most common periodontal pathogen among the periodontally healthy children 9.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Topçuoğlu

Çıftçı²,

Paltura³

et al. 2012

Int. J. Med. Sci.

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Objective: In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of Fusobacterium nucleatum and Treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME.Methods: Totally 20 children with OME undergoing myringotomy and ventilation tube placement were attended. Stimulated saliva samples were collected after otorhinolaryngological and oral examinations were done. The middle ear effusion and nasopharyngeal secretions were collected during the operations. The presence of F. nucleatum and T. denticola were detected using 16SrRNA-based PCR. The clonal similarities of the bacteria were detected in the samples which the same bacteria had been detected in each samples of the same child. After DNA sequencing, clonal similarity was determined by 16SrRNA gene clone library analysis. The sequences from each clone were compared with similar sequences of reference organisms by FASTA search.Results: T. denticola was detected only in four (20%) saliva and in one (5%) nasopharyngeal sample. F. nucleatum was detected in 11 (55%) saliva, eight (40%) nasopharyngeal and six (30%) middle ear effusion samples. Sequences from F.nucleatum clones derived from three different anatomic sites within patients were similar in 33% of OME patients, indicating their genetic relatedness.Conclusions: Bacteria involved in this process most likely originate from the oropharynx since they show a close genetic relatedness with their oropharyngeal counterparts.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Topçuoğlu

Çıftçı²,

Paltura³

et al. 2012

Int. J. Med. Sci.

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“…Table 1 lists the species‐specific PCR primers chosen based on previously published sequences 27,32 . The PCR was performed according to a previously described protocol 33 . PCR mix contained 1 mM of each primer, 1.5 mM MgCl 2 , 200 mM of deoxyribonucleotide triphosphate, §§ 1× Taq polymerase buffer, ‖‖ 1.25 units of Taq DNA polymerase, and 10 ml of template DNA in 50‐μl reaction volume.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Amoxicillin and Metronidazole Combination for the Management of Generalized Aggressive Periodontitis

Yek

Çintan

Topçuoğlu

et al. 2010

Journal of Periodontology

125

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The results from this study suggest that combined amoxicillin and metronidazole use as an adjunct to scaling and root planing leads to better clinical healing compared to mechanical treatment alone. The polypharmaceutical approach used results in a significant and substantial decrease in T. forsythia and prevents its recolonization for 6 months, suggesting that T. forsythia may determine the long-term stability of periodontal treatment outcomes.

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“…This is mainly because conventional identification of anaerobes is a complicated, expensive and time‐consuming process and it is often not reliable, because it is based on outdated taxonomy (Song 2005). Conversely, genetic‐based methods are among the most sensitive and specific tools for identifying bacteria especially with clinical purposes (Bolstad and Jensen 1993; Külekçi et al. 2001; Haraldsson et al.…”

Section: Discussionmentioning

confidence: 99%

“…Isolated DNA was used as template for Fus. nucleatum identification by employing primers targeting 16S rRNA gene sequences (Külekçi et al. 2001).…”

Section: Methodsmentioning

confidence: 99%

Physiological alterations of a Fusobacterium nucleatum strain exposed to oxidative stress

et al. 2007

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Aim: The purpose of this study was to investigate the effect of oxidative stress on physiological and genetic characteristics of Fusobacterium nucleatum and its interference on this microbial identification methods. Methods and Results: Fus. nucleatum ssp. nucleatum ATCC 25586 (wt‐strain) and an oxidative‐stress‐adapted strain derived from the wt‐strain (aero‐strain) were employed in the study. Cell‐free crude protein extracts were obtained from both strains and differentially expressed proteins were identified by two‐dimensional electrophoresis. Bacterium identification was performed by conventional biochemical tests, automated Rapid ID 32A system and specific PCR analysis. Genetic diversity between wt‐ and aero‐strain was assessed by arbitrarily‐primed (AP)‐PCR. There were significant changes in the protein profile of aero‐strain. The identification of the wt‐strain was confirmed by all methods employed. Similar results were obtained for aero‐strain when conventional biochemical tests and PCR were used. However, aero‐strain was identified as Fusobacterium varium when submitted to Rapid ID 32A system. According to AP‐PCR analysis, no significant genetic alteration was detected in aero‐strain. Conclusions: The adaptive response of Fus. nucleatum to oxidative stress is associated with changes on its biology, which may lead to misidentification of the organism, according to the conventional identification methods. Significance and Impact of the Study: Oxidative stress may act as a cause of adaptive response in Fus. nucleatum with consequences to its biology, such as alterations on biochemical and physiological profile.

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PCR analysis of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola andFusobacterium nucleatum in middle ear effusion

Cited by 9 publications

References 31 publications

Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Efficacy of Amoxicillin and Metronidazole Combination for the Management of Generalized Aggressive Periodontitis

Physiological alterations of a Fusobacterium nucleatum strain exposed to oxidative stress

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