PCR Analysis of Nasal Polyps, Chronic Sinusitis, and Hypertrophied Turbinates for DNA Encoding Bacterial 16S rRNA

Bucholtz, Gerald A.; Salzman, Sherry A.; Bersalona, Fernando; Boyle, Timothy R.; Ejercito, Victor S.; Penno, Linda; Peterson, Douglas W; Stone, Gwen E.; Urquhart, Andrew C.; Shukla, Sanjay Kumar; Burmester, James K.

doi:10.1177/194589240201600309

Cited by 29 publications

(18 citation statements)

References 18 publications

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“…Streptococcus spp . DNA was seen inside the tissue in one polyp sample and Pseudomonas aeruginosa DNA was isolated from one maxillary sinus biopsy[57]. These negative results could be due to the sampling and analysis methods used in this study.…”

Section: Bacterial Microbiomementioning

confidence: 97%

A comprehensive review of the nasal microbiome in chronic rhinosinusitis (CRS)

Mahdavinia

Keshavarzian

Tobin

et al. 2015

Clin Experimental Allergy

131

126

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Chronic rhinosinusitis (CRS) has been known as a disease with strong infectious and inflammatory components for decades. The recent advancement in methods identifying microbes has helped implicate the airway microbiome in inflammatory respiratory diseases such as asthma and COPD. Such studies support a role of resident microbes in both health and disease of host tissue, especially in the case of inflammatory mucosal diseases. Identifying interactive events between microbes and elements of the immune system can help us to uncover the pathogenic mechanisms underlying chronic rhinosinusitis. Here we provide a review of the findings on the complex upper respiratory microbiome in CRS in comparison to healthy controls. Furthermore, we have reviewed the defects and alterations of the host immune system that interact with microbes and could be associated with dysbiosis in CRS.

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Section: Bacterial Microbiomementioning

confidence: 97%

A comprehensive review of the nasal microbiome in chronic rhinosinusitis (CRS)

Mahdavinia

Keshavarzian

Tobin

et al. 2015

Clin Experimental Allergy

131

126

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“…Even PCR techniques have failed to demonstrate bacterial infection in most cases (Bucholtz et al, 2002). Hamilos et al (1993), who obtained antral cultures from 12 subjects with chronic maxillary sinusitis with nasal polyps, isolated organisms from only three patients.…”

Section: Introductionmentioning

confidence: 99%

Bacteriology of chronic maxillary sinusitis associated with nasal polyposis

Brook

Frazier

2005

Journal of Medical Microbiology

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Aspirates from 48 chronically inflamed maxillary sinuses from patients who had nasal polyposis were processed for aerobic and anaerobic bacteria. Bacterial growth was present in 46 (96 %) specimens. Aerobic or facultative bacteria were present in 6 (13 %) specimens, anaerobic bacteria alone in 18 (39 %), and mixed aerobic and anaerobic bacteria in 22 (48 %). There were 110 bacterial isolates (2 . 4 per specimen). Thirty-nine of the isolates were aerobic or facultative organisms (0 . 85 per specimen). The predominant aerobic or facultative organisms were: Staphylococcus aureus, microaerophilic streptococci, Haemophilus influenzae and Moraxella catarrhalis. Seventy-one anaerobes were isolated (1 . 5 per specimen), Peptostreptococcus spp., Prevotella spp., Porphyromonas asaccharolytica and Fusobacterium spp. being predominant. These findings illustrate for the first time the presence of polymicrobial aerobic-anaerobic flora in patients with chronic maxillary sinusitis who had nasal polyposis.

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“…The role of bacterial colonization in the pathogenesis of CRS is unclear, with some studies reporting sterile findings on culture of samples from affected patients [5,6] and others reporting positive bacterial culture rates of 30-100% [7]. These differences may be attributable to differences in sinus sampling methods, lack of standardization in obtaining samples, and variations in bacterial growth methods [7].…”

Section: Introductionmentioning

confidence: 99%

Bacteriology of the Middle Meatus in Chronic Rhinosinusitis with and without Polyposis

Stern

Hadar²,

Nachalon³

et al. 2016

ORL

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There are only limited data in the literature, and none specifically from the Middle East, on the pathogenic bacteria in chronic rhinosinusitis (CRS) as opposed to healthy nasal cavities and their association with disease severity. The present study was conducted in the department of head and neck surgery of a tertiary medical center. Middle meatal swabs were taken preoperatively from patients with CRS with nasal polyposis (CRSwNP) (n = 60), CRS without nasal polyposis (CRSsNP) (n = 50), and control patients with septal deviation (n = 26) or no nasal abnormalities (n = 27). Culture findings were compared among the groups and correlated with CRS severity. Positive pathogenic culture rates were 78% in the CRSwNP group and 64% in the CRSsNP group. Twenty pathogenic bacterial species were identified; the most common was Staphylococcus aureus (27%). The most common Gram-negative isolate was Citrobacter spp. (17%). Gram-negative species were significantly more prevalent in the CRSwNP group than the others. Mean Lund-Mackay scores were 12.8 in the CRSwNP group and 6.9 in the CRSsNP group, and were unrelated to the culture findings. Positive culture rates were significantly higher in the septal deviation (54%) than the nasal healthy group (26%), although both values were significantly lower than in the CRS groups. In conclusion, patients with CRS have higher rates of bacterial isolates than patients without CRS. CRSwNP is associated with more Gram-negative bacteria than CRSsNP, regardless of disease severity. The relatively high positive culture rate in patients with septal deviation merits investigation.

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PCR Analysis of Nasal Polyps, Chronic Sinusitis, and Hypertrophied Turbinates for DNA Encoding Bacterial 16S rRNA

Abstract: This study suggests that chronic bacterial infection is not a major component of nasal polyp etiology.

Cited by 29 publications

References 18 publications

A comprehensive review of the nasal microbiome in chronic rhinosinusitis (CRS)

A comprehensive review of the nasal microbiome in chronic rhinosinusitis (CRS)

Bacteriology of chronic maxillary sinusitis associated with nasal polyposis

Bacteriology of the Middle Meatus in Chronic Rhinosinusitis with and without Polyposis

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