PCR analysis of Pasteurella multocida isolates from an outbreak of pasteurellosis in Indian pigs

Kalorey, D. R.; Yuvaraj, Samrithi; Vanjari, S.S.; Gunjal, P.S.; Dhanawade, Nirmala B.; Barbuddhe, Sukhadeo B.; Bhandarkar, A. G.

doi:10.1016/j.cimid.2007.06.003

Cited by 19 publications

(19 citation statements)

References 14 publications

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“…Also, with both injection routes, an AUC of almost 12 μg h/mL was determined for both of tissue‐cage fluids. The AUC ratios relative to serum were approximately 0.9:1.0, which was higher than those described in dairy calves (approximately 0.8:1.0) (Kalorey et al ., ), but lower than those in sheep (approximately 1.3:1.0) (Sidhu et al ., ).…”

Section: Discussionmentioning

confidence: 82%

Pharmacokinetic/pharmacodynamic relationship of marbofloxacin against Pasteurella multocida in a tissue‐cage model in yellow cattle

Wang

Yang

et al. 2013

Vet Pharm & Therapeutics

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The fluoroquinolone antimicrobial drug marbofloxacin was administered to yellow cattle intravenously and intramuscularly at a dose of 2 mg/kg of body weight in a two-period crossover study. The pharmacokinetic properties of marbofloxacin in serum, inflamed tissue-cage fluid (exudate), and noninflamed tissue-cage fluid (transudate) were studied by using a tissue-cage model. The in vitro and ex vivo activities of marbofloxacin in serum, exudate, and transudate against a pathogenic strain of Pasteurella multocida (P. multocida) were determined. Integration of in vivo pharmacokinetic data with the in vitro MIC provided mean values for the area under the curve (AUC)/MIC for serum, exudate, and transudate of 155.75, 153.00, and 138.88, respectively, after intravenous dosing and 160.50, 151.00, and 137.63, respectively, after intramuscular dosing. After intramuscular dosing, the maximum concentration/MIC ratios for serum, exudate, and transudate were 21.13, 9.13, and 8.38, respectively. The ex vivo growth inhibition data after intramuscular dosing were fitted to the inhibitory sigmoid Emax equation to provide the values of AUC/MIC required to produce bacteriostasis, bactericidal activity, and elimination of bacteria. The respective values for serum were 17.25, 31.29, and 109.62, and slightly lower values were obtained for transudate and exudate. It is proposed that these findings might be used with MIC50 or MIC90 data to provide a rational approach to the design of dosage schedules which optimize efficacy in respect of bacteriological as well as clinical cures.

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Section: Discussionmentioning

confidence: 82%

Pharmacokinetic/pharmacodynamic relationship of marbofloxacin against Pasteurella multocida in a tissue‐cage model in yellow cattle

Wang

Yang

et al. 2013

Vet Pharm & Therapeutics

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“…For environmental samples, our group had previously reported the presence of this pathogen in drinking water samples from swine farms where this pathogen could be isolated and observed that these environmental isolates could present biofilm formation as a means of survive outside the pig [22]. On the other hand, P. multocida is known as part of the normal flora of the pig, as well as the causal agent of wide variety of infections, usually after primary infections caused by other pathogens or environmental factors [45]- [47]. We identified toxigenic strains in all kind of environmental samples, nasal swab samples and urine samples.…”

Section: Discussionmentioning

confidence: 99%

Porcine Respiratory Pathogens in Swine Farms Environment in Mexico

Loera-Muro¹,

Loera‐Muro²,

Morfín-Mata³

et al. 2014

OJAS

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Respiratory pathogens are the main health problem in the swine industry worldwide. These pathogens are transmitted by direct contact between animals or by aerosols and however are not well known yet, if the environment works as its reservoir, inoculum and/or dispersion medium. The objective of this study was to determine the presence of respiratory pathogens in environmental samples from swine farms in Aguascalientes, Mexico, through of PCR and RT-PCR techniques. The bacteria Actinobacillus pleuropneumoniae and Pasteurella multocida were found viable in samples from water, food, soil and air. Streptococcus suis was found in a viable state in water samples. Haemophilus parasuis, Porcine Reproductive and Respiratory Syndrome virus and Swine Influenza virus (H1N1 and H3N2) were detected in drinking water samples. Mycoplasma hyopneumoniae and Porcine Circovirus type 2 (PCV2) were not detected in environmental samples. These results suggest that the environment of the farms acts as a reservoir, inoculum and/or vehicle of dispersion for these pathogens except for M. hyopneumoniae and PCV2.

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“…The economic catastrophe caused by this pathogen on Indian livestock industry can be guessed from the fact that only haemorrhagic septicaemia (HS) causes 46–55% of all bovine deaths (Dutta et al., ). Other than HS, the bacteria has been regularly isolated from sporadic septicaemic cases in pigs and other ruminants (De Alwis, ; Verma, ), from pneumonia cases of pigs (Kalorey et al., ) and from fowl cholera cases of avian (Shivachandra et al., ). The organism is a diverse and complex bacteria with respect to antigenic variation, host predilection and pathogenesis (Carter and Chengappa, ; Hunt et al., ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology ofPasteurella multocidaCirculating in India by Multilocus Sequence Typing

Sarangi

Thomas

Gupta

et al. 2014

Transbound Emerg Dis

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Multilocus sequence typing (MLST), a sequence-based typing method for bacterial pathogens, is currently the best method for long-term epidemiological study and to understand the population structure of the bacteria. This investigation was carried out to study the diversity of Pasteurella multocida isolates circulating in India. Ten different sequence types (ST) identified in this study are ST 122 from cattle, goat, mithun and pig; ST 50 from pig; ST 9 from cattle and sheep; ST 229 from cattle and goat; ST 71 and ST 277 from cattle; and ST 129, ST 280, ST 281 and ST 282 from avian species. Of these, ST 277, ST 280, ST 281 and ST 282 were identified for the first time. The analysis of results provides novel epidemiological information on the circulation of multiple STs across India. The majority of STs or their variants identified in this study have already been reported from different parts of the globe. This suggests that probably transboundary spread of strains across countries and continents has occurred across evolutionary time and is still happening. The isolation of ST 122 from small ruminants and pigs suggests that these species may be included in the preventive vaccination policy for effective control of haemorrhagic septicaemia in India.

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PCR analysis of Pasteurella multocida isolates from an outbreak of pasteurellosis in Indian pigs

Cited by 19 publications

References 14 publications

Pharmacokinetic/pharmacodynamic relationship of marbofloxacin against Pasteurella multocida in a tissue‐cage model in yellow cattle

Pharmacokinetic/pharmacodynamic relationship of marbofloxacin against Pasteurella multocida in a tissue‐cage model in yellow cattle

Porcine Respiratory Pathogens in Swine Farms Environment in Mexico

Molecular Epidemiology ofPasteurella multocidaCirculating in India by Multilocus Sequence Typing

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