PCR Analysis of the Tri13 gene to Determine the Genetic Potential of Fusarium graminearum Isolates from Iran to Produce Nivalenol and Deoxynivalenol

Abstract

Fusarium graminearum trichothecene producing isolates can be broadly divided into two chemotypes based on the production of the 8- ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). Functional Tri13 gene required for the production of NIV and 4- acetyl NIV, whereas in the isolates producing DON and its acetylated derivates, this gene is nonfunctional. In this study, a total of 57 isolates from different fields of Mazandaran province, Iran were identified as F. graminearum using classical methods and species specific primers. In order to assess the potential of isolates to produce NIV or DON, we used PCR to determine whether isolates carried a functional or nonfunctional Tri13 gene. Out of the 57 tested F. graminearum isolates with Tri13 PCR assays, 46 yielded an amplicon similar to the size predicted for nivalenol production, while 11 yielded an amplicon similar to the size predicted for deoxynivalenol production. From regions where more than one F. graminearum isolate was obtained, isolates were not exclusively of a single chemotype. It seems that genetic diversity among the isolates has relation with geographical region and wheat cultivar. The assay can provide information about the distribution of Tri13 haplotype that can be used in tracing of trichothecene contaminated samples.

Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA

“…These markers have also provided knowledge about nucleotide sequence homology and orthologous genes among genomes (Nicholson et al, 1998;Adinolfi et al, 2007;Saharan et al, 2007). They are frequently used in the molecular characterization of Fusarium at the genus and species levels (Schilling et al, 1996;Haratian et al, 2008).…”

“…In addition to their carcinogenic effects, they inhibit ribosomal protein synthesis (Gutleb et al, 2002). Research has recently focused on the molecular characterization of causal agents and the production of mycotoxins to improve pathogen recognition (Saharan et al, 2007;Haratian et al, 2008;Pandolfi et al, 2010).…”

Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland

“…Such a wide F. poae occurrence is unfavorable, as this species belongs to the causal agents of Fusarium ear rot (Chełkowski et al 2007), which is one of the most important maize diseasesdecreasing yield and affecting grain quality and feed value of the grain (Czembor et al 2010;Rahjoo et al 2008). Haratian et al (2008) applied the positive-negative PCR assay based on the Tri13 gene to identify the genetic potential of DON and NIV production of F. graminearum. In this study, only 11 Fusarium spp.…”

“…In other species detected by these authors, the NIV chemotype was also sporadically detected (representing only 5.8% of the total population). Haratian et al (2008) revealed that both DON and NIV Fusarium spp. chemotypes existed in Iran, but NIV producers were more frequently reported.…”

Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays

“…For chemotype identification, there is a rapid procedure based on the amplification of specific genes encoding for proteins involved in mycotoxin biosynthesis. Genes such as: TRI2, TRI3, TRI5, TRI6, TRI7, TRI13 and TRI101 are encoded for biosynthesis of trichothecene and can be assayed by PCR to identify the ability of trichothecene biosynthesis and also chemotypes of the fungi (Chandler et al 2003;Haratian et al 2008;Sampietro et al 2010;Yoruk et al 2016).…”