PCR and RAPD identification of<i>L. plantarum</i>strains isolated from ovine milk and cheese. Geographical distribution of strains

Oneca, M.; Irigoyen, A.; Ortigosa, M.; Torre, P.

doi:10.1016/s0378-1097(03)00691-8

Cited by 32 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, ANOVA did not reveal any significant relationship between the percentage of the two subspecies and the origin of the milk samples. This finding agrees with the observations published by Oneca et al (2003) on wild Lactobacillus plantarum populations of raw milks produced in the Roncal PDO area but disagrees with those of Corroler et al (1998) who reported that 80% of isolates from the Bocage Falaisien area in Normandy were members of subsp. lactis genotype, while 70% of the Bessin isolates were members of subsp.…”

Section: Genotypic Diversity Of Strains Of L Lactissupporting

confidence: 77%

Lactococcus lactis strains from raw ewe’s milk samples from the PDO Ossau-Iraty cheese area: levels, genotypic and technological diversity

Feutry¹,

Torre

Arana

et al. 2012

Dairy Sci. & Technol.

Self Cite

View full text Add to dashboard Cite

The aims of this work were to assess the levels of Lactococcus lactis (L. lactis) in ewe's milk produced in three Ossau-Iraty cheese sub-areas and to investigate the genotypic and technological diversity of isolated wild strains of L. lactis in order to assess their suitability for use as components of starter formulations. Thirty-two milk samples from 32 farms were collected. Strains of L. lactis were identified and quantified using a combination of species and subspecies-specific polymerase chain reaction (PCR) and PCR amplification of repetitive bacterial DNA elements (Rep-PCR). The genotypic and technological diversity of the indigenous strains was compared to that of 12 commercial strains. L. lactis was detected in milk samples from only 20 farms. The levels detected were below 4 log 10 cfu.mL −1 in 75% of the milks. L. lactis subsp. lactis dominated in 66% of the samples. Forty-three genotypic profiles of wild L. lactis strains were detected and showed greater diversity than those of the commercial strains. Milks containing L. lactis contained one to four distinct strains. With the exception of two strains, each strain was found in milk from only one farm. The Prt + strains were the most acidifying. Sensitivity to phages collected from wheys differed widely between the commercial (60%) and indigenous strains (5%). Wild strains of L. lactis displayed a wide genotypic and technological diversity. Genotypic diversity seemed to be linked to the farm of origin. This study addresses questions regarding the environmental factors which influence such natural diversity. A deeper knowledge of the strain-dependent technological properties would be useful in selecting strains for use in starter blends.

show abstract

Section: Genotypic Diversity Of Strains Of L Lactissupporting

confidence: 77%

Lactococcus lactis strains from raw ewe’s milk samples from the PDO Ossau-Iraty cheese area: levels, genotypic and technological diversity

Feutry¹,

Torre

Arana

et al. 2012

Dairy Sci. & Technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The specific environmental conditions prevailing in a fermenting food substrate promote the growth of some of these bacteria. Lactobacillus plantarum is predominantly found in fermented food and feed products, and is implicated in the processing of food for human consumption, such as sauerkraut, dry fermented sausage, wine and green olive fermentations and cheese making (Ruiz-Barba et al, 1994;Oneca et al, 2003), as well as in animal nutrition, such as crop preservation (Merry et al, 1995), fish and crab waste fermentation (Abazinge et al, 1993) and poultry by-product fermentation (Urlings et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…Genotypic methods used for L. plantarum strain typing are typically PCR-based methods, for example randomly amplified polymorphic DNA (Johansson et al, 1995;Oneca et al, 2003;Elegado et al, 2004) or variations of restriction enzyme analysis, for example ribotyping (Yansanjav et al, 2003;Rodas et al, 2005) and pulsed-field gel electrophoresis (Sánchez et al, 2004). Multilocus sequence typing (MLST) has recently been shown to be a powerful technique for bacterial typing.…”

Section: Introductionmentioning

confidence: 99%

Development of a multilocus sequence typing method for analysis of Lactobacillus plantarum strains

2006

View full text Add to dashboard Cite

Lactobacillus plantarum is a species of considerable industrial and medical interest. To date, the lack of reliable molecular methods for definite identification at strain level has hindered studies of the population biology of this organism. Here, a multilocus sequence typing (MLST) system for this organism is described, which exploits the genetic variation present in six housekeeping loci to determine the genetic relationship among isolates. The MLST system was established using 16 L. plantarum strains that were also characterized by ribotyping and restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified 16S-23S rDNA intergenic spacer region (ISR). Ribotyping grouped the strains into four groups; however, RFLP analysis of the ISRs showed no differences in the strains analysed. In contrast, MLST had a good discriminatory ability. The sequence analysis of the six genes showed 14 different allelic combinations, with 12 of them represented by only one strain. By using this MLST approach we were able to confirm the identity of two strains deposited in the Spanish Type Culture Collection as different strains. Phylogenetic analysis indicated a panmictic population structure of L. plantarum and split decomposition analysis indicated that recombination plays a role in creating genetic heterogeneity in L. plantarum. As MLST allows precise identification, and easy comparison and exchange of results obtained in different laboratories, the future application of this new molecular method could be useful for the identification of valuable L. plantarum strains.

show abstract

“…Lactobacillus plantarum is a common inhabitant of the human gastrointestinal tract (3), but it is also encountered in a variety of environmental niches, such as dairy, meat, and vegetable fermentations (25,39). L. plantarum is widely used in industrial and traditional production of fermented plant, food, and feed products, such as sauerkraut, sausage, cabbage, olives, and silage (14,31,41).…”

mentioning

confidence: 99%

Indigenous and Environmental Modulation of Frequencies of Mutation in Lactobacillus plantarum

Machielsen

Alen-Boerrigter

Koole

et al. 2010

Appl Environ Microbiol

View full text Add to dashboard Cite

Reliability of microbial (starter) strains in terms of quality, functional properties, growth performance, and robustness is essential for industrial applications. In an industrial fermentation process, the bacterium should be able to successfully withstand various adverse conditions during processing, such as acid, osmotic, temperature, and oxidative stresses. Besides the evolved defense mechanisms, stress-induced mutations participate in adaptive evolution for survival under stress conditions. However, this may lead to accumulation of mutant strains, which may be accompanied by loss of desired functional properties. Defining the effects of specific fermentation or processing conditions on the mutation frequency is an important step toward preventing loss of genome integrity and maintaining the productivity of industrial strains. Therefore, a set of Lactobacillus plantarum mutator reporter strains suitable for qualitative and quantitative analysis of lowfrequency mutation events was developed. The mutation reporter system constructed was validated by using chemical mutagenesis (N-methyl-N-nitro-N-nitrosoguanidine) and by controlled expression of endogenous candidate mutator genes (e.g., a truncated derivative of the L. plantarum hexA gene). Growth at different temperatures, under low-pH conditions, at high salt concentrations, or under starvation conditions did not have a significant effect on the mutation frequency. However, incubation with sublethal levels of hydrogen peroxide resulted in a 100-fold increase in the mutation frequency compared to the background mutation frequency. Importantly, when cells of L. plantarum were adapted to 42°C prior to treatment with sublethal levels of hydrogen peroxide, there was a 10-fold increase in survival after peroxide treatment, and there was a concomitant 50-fold decrease in the mutation frequency. These results show that specific environmental conditions encountered by bacteria may significantly influence the genetic stability of strains, while protection against mutagenic conditions may be obtained by pretreatment of cultures with other, nonmutagenic stress conditions.

show abstract

PCR and RAPD identification ofL. plantarumstrains isolated from ovine milk and cheese. Geographical distribution of strains

Cited by 32 publications

References 27 publications

Lactococcus lactis strains from raw ewe’s milk samples from the PDO Ossau-Iraty cheese area: levels, genotypic and technological diversity

Lactococcus lactis strains from raw ewe’s milk samples from the PDO Ossau-Iraty cheese area: levels, genotypic and technological diversity

Development of a multilocus sequence typing method for analysis of Lactobacillus plantarum strains

Indigenous and Environmental Modulation of Frequencies of Mutation in Lactobacillus plantarum

Contact Info

Product

Resources

About