PCR array and protein array studies demonstrate that IL-1<b>β</b>(interleukin-1<b>β</b>) stimulates the expression and secretion of multiple cytokines and chemokines in human adipocytes

Alomar, Suliman Yousef; Zaïbi, Mohamed S.; Kępczyńska, Małgorzata A.; Gentili, Alessandra; Alkhuriji, Afrah Fahad; Mansour, Lamjed; Dar, Javid A.; Trayhurn, Paul

doi:10.3109/13813455.2015.1087034

Cited by 18 publications

(46 citation statements)

References 38 publications

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“…expression is also strongly up-regulated in human adipocytes by IL-1β [14]. The substantial increase in G-CSF production may be linked to neutrophil recruitment and function in adipose tissue, as well as a potential neutrophic/neuroprotective role in inflammation in the tissue.…”

Section: Discussionmentioning

confidence: 94%

“…Since adiponectin, the adipocyte hormone encoded by ADIPOQ, has antiinflammatory and insulin sensitising functions [see 7], IL-33 does not compromise the production of a factor with these actions, unlike IL-1β and TNFα which inhibit ADIPOQ expression [7,14]. As with GPR120, the possibility cannot be excluded of some small response to IL-33 at time-points intermediate to 3 and 24 h.…”

Section: Discussionmentioning

confidence: 99%

“…The cells were plated into 12-well plates (5,000 cells/cm 2 ), cultured to confluence, differentiated into adipocytes and then further cultured in nutrition medium (changed every 2-3 days) for up to 14 days, as previously [14]. The nutrition medium contained 3% foetal calf serum, dexamethasone (400 ng/ml) and insulin (0.5 μg/ml).…”

Section: Adipocyte Cell Culturementioning

confidence: 99%

“…Data were analysed by the comparative 2 -ΔΔ Ct method, as previously [7,14] and expressed as fold-changes in the target gene (normalized to ACTB) in adipocytes treated with IL-33 in relation to expression in the control cells (control cells =1).…”

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

“…Plates were pre-coated with antibodies on discrete spots and the assay performed according to the manufacturer's instructions, as previously [7,14]; data were analysed using Meso Scale software. The minimum level of detection was 0.06 pg/ml for IL-6 (interlot CV <20 %) and 1.5 pg/ml for CSF3 (interlot CV <18%).…”

Section: Protein Immunoassaysmentioning

confidence: 99%

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IL-33 stimulates expression of the GPR84 (EX33) fatty acid receptor gene and of cytokine and chemokine genes in human adipocytes

et al. 2018

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Expression of GPCR fatty acid sensor/receptor genes in adipocytes is modulated by inflammatory mediators, particularly IL-1β. In this study we examined whether the IL-1 gene superfamily member, IL-33, also regulates expression of the fatty acid receptor genes in adipocytes. Human fat cells, differentiated from preadipocytes, were incubated with IL-33 at three different dose levels for 3 or 24 h and mRNA measured by qPCR. Treatment with IL-33 induced a dose-dependent increase in GPR84 mRNA at 3 h, the level with the highest dose being 13.7-fold greater than in controls. Stimulation of GPR84 expression was transitory; the mRNA level was not elevated at 24 h. In contrast to GPR84, IL-33 had no effect on GPR120 expression. IL-33 markedly stimulated expression of the IL1B, CCL2, IL6, CXCL2 and CSF3 genes, but there was no effect on ADIPOQ expression. The largest effect was on CSF3, the mRNA level of which increased 183-fold over controls at 3 h with the highest dose of IL-33;there was a parallel increase in the secretion of G-CSF protein into the medium. It is concluded that in human adipocytes IL-33, which is synthesised in adipose tissue, has a strong stimulatory effect on the expression of cytokine and chemokine genes, particularly CSF3, and on the expression of GPR84, a pro-inflammatory fatty acid receptor.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Adipocyte Cell Culturementioning

confidence: 99%

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

Section: Protein Immunoassaysmentioning

confidence: 99%

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IL-33 stimulates expression of the GPR84 (EX33) fatty acid receptor gene and of cytokine and chemokine genes in human adipocytes

et al. 2018

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Ginger and avocado as nutraceuticals for obesity and its comorbidities

Tramontin

Luciano

Marques

et al. 2020

Phytotherapy Research

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Obesity is a worldwide epidemic and is one of the factors involved in the etiology of type 2 diabetes mellitus. Obesity induces low-grade inflammation and oxidative stress. The treatment for obesity involves changes in diet, physical activity, and even medication and surgery. Currently, the use of nutraceutical compounds is associated with health benefits. Ginger and avocado are used for many people all around the world; however, its effect as a nutraceutical compound is less known by the general population. For this reason, we searched information of the literature to point its effects on distinct mechanisms of defense against the obesity its comorbidities. The present review aimed showing that these nutraceuticals may be useful in obesity treatment. Reports have shown that ginger and avocado induce antioxidant and antiinflammatory effects by improving enzymatic activity and modulating obesity-related impairments in the anti-inflammatory system in different tissues, without side effects.Furthermore, ginger and avocado were found to be effective in reversing the harmful effects of obesity on blood lipids. In conclusion, on the basis of the positive effects of ginger and avocado in in vitro, animal, and human studies, these nutraceuticals may be useful in obesity treatment. K E Y W O R D Sdiabetes treatment, inflammation, obesity, oxidative stress, phytotherapeutic approach, unhealthy diet

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