PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings

Abstract

The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control and treated groups at each of the four sampling hours. A total of six selected RAPD primers were used for PCR amplification. Template stability has been taken as the measure of DNA damage caused by pesticide. The results obtained showed no significant difference in the template stability in the blood DNA of furadan treated groups at any of the four sampling hours; however, the liver DNA were able to show significant difference at 48 and 96 hours of treatment.

Freshwater toxic cyanobacteria induced DNA damage in apple (Malus pumila), rape (Brassica napus) and rice (Oryza sativa)

“…RAPD is a PCR-based technique that has been widely used to detect DNA damage caused by various environmental pollutants and mutagens such as heavy metal ions [26,27], pesticide [28] and radiation [29,30]. This is the first report that we know of that employs RAPD assay for evaluating genotoxic effects of microcystins.…”

Genotoxicity Testing in Pesticide Safety Evaluation

“…Many reviews details the employment of comet assay in the assessment of the genotoxic potential of many compounds, which notably include metals, pesticides, opiates, nitrosamines and anticancer drugs (Collins, 2004). A significant advantage of the comet assay is its applicability to any eukaryotic organism and nucleated cell type (Mohanty et al, 2009a(Mohanty et al, , 2009b(Mohanty et al, , 2011. This assay can be applied both in vitro and in vivo conditions after an exposure to different potent genotoxic and mutagenic agents (.…”

“…A study was carried out by Mohanty et al, (2009aMohanty et al, ( , 2009bMohanty et al, ( and 2011 with an objective to study the genotoxic effects of two pesticides, phorate, an organophosphate and furadan, a carbamate on rohu (Labeo rohita) fingerlings. To evaluate the DNA damage, rohu fingerlings were exposed to control, 0.001, 0.002 and 0.01ppm of phorate control, 0.002, 0.004 and 0.02ppm of furadan for a total time period of 96h.…”

Genotoxicity assessment of pesticides on terrestrial snail embryos by analysis of random amplified polymorphic DNA profiles

“…RAPD has been successfully used on many organisms, such as plants [5][6][7], algae [8,9], and fish [10][11][12][13][14]. This technique has been used to detect signs of genotoxicity from various contaminants, including non-essential metals, such as cadmium, boron, arsenic, mercury, nickel, and aluminum [15][16][17][18][19], and essential metals, such as zinc, sodium, manganese, and copper [6,7,9,[20][21][22].…”

“…RAPD has also been used to demonstrate the genotoxicity of various physical compounds, such as UV A and B [8] and gamma rays [23], the radioactivity of uranium and tritium [24,25] and emergent pollutant nanoparticles, such as ZnO and CuO [26]. The genotoxicity of organic compounds, such as drugs [27,29] or herbicides [11,[30][31][32] has also been largely studied. However, there have been few reports using RAPD to detect DNA damage in embryos and terrestrial animals.…”