PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil

Oliveira, Fernanda Santos de; Pirmez, Claude; Pires, Marize Quinhones; Brazil, Reginaldo P.; Pacheco, Raquel S.

doi:10.1016/j.vetpar.2005.01.005

Cited by 97 publications

(82 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibody titers ≥ to 10 were detected in four (100%) crab-eating foxes (Cerdocyon Difficulties with wild animal capture, taxonomic identification and standardization of laboratory techniques for ACL diagnosis constitute restricting factors in the understanding of leishmaniasis epidemiology (17). Several authors, employing Leishmania promastigotes, had utilized DAT in epidemiological studies (32)(33)(34)(35).…”

Section: Search For Antibodies By Datmentioning

confidence: 99%

“…Leishmania (L.) mexicana has been found in big-eared climbing rats (Ototylomys phyllotis), blackeared rice-rats (Oryzomys melanotis), Yucatan deer-mice (Peromyscus yucatanicus) and hispid cotton rats (Sigmodon hispidus) (12). Leishmania parasites have been found in armadillos (Dasypus novemcinctus), foxes (Lycalopex vetulus) and in small rodents (Akodon arviculoides and Oryzomys nigripes) (9,13,14); while Leishmania (Viannia) braziliensis has also been isolated from black rats (Rattus rattus) (5,(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Procyon cancrivorus and Nasua nasua. These results suggest that Cerdocyon thous and Lycalopex vetulus (crab-eating fox), Cebus apella (capuchin monkey), Dasyprocta azarae (agouti), Procyon cancrivorus (crab-eating raccoon) and Nasua nasua (coati) play an important role in the ACL transmission cycle in the northwestern region of Paraná, Brazil.

show abstract

Section: Search For Antibodies By Datmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Voltarelli

Arraes

Perles

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…Agroecosystems are mosaic landscapes, heterogeneous in space and time, which may cause significant changes in the small mammal assemblage that occupies the area in relation to diversity, demography and habitat use (Utrera et al, 2000). Furthermore, rural environments are of special interest to public health because of the transmission of several zoonoses from wildlife to humans, where small mammal species can act as reservoirs of hantavirus disease (Mills and Child, 1998;Padula et al, 2004;Suzuki et al, 2004), hemorrhagic fevers (Mills et al, 1992b), trypanosomiasis (Herrera et al, 2004;Herrera et al, 2005), leishmaniasis (Oliveira et al, 2005) and schistosomiasis (Rey, 1993;D'Andrea et al, 2000). Moreover, reservoir populations may reach high densities in an "explosive" fashion ("ratada"), making them agricultural pests responsible for heavy economic losses in most of the Neotropics (Rodríguez, 1993).…”

Section: Introductionmentioning

confidence: 99%

Small mammal populations of an agroecosystem in the Atlantic Forest domain, southeastern Brazil

D’Andrea

Gentile

et al. 2007

Braz. J. Biol.

View full text Add to dashboard Cite

This study reports 2 years of the population dynamics and reproduction of a small mammal community using the removal method. The study was conducted in a rural area of the Atlantic Forest, in Sumidouro, Rio de Janeiro State, Brazil. The population sizes, age structure and reproduction were studied for the four most common species in the study area. The overall diversity was 1.67 and ranged between 0.8 to 1.67. The species richness was 13 considering the whole study. The most abundant species were the rodents Nectomys squamipes (n = 133), Akodon cursor (n = 74), Oligoryzomys nigripes (n = 25) and the marsupials Didelphis aurita (n = 58) and Philander frenatus (n = 50). Seven other rodents were captured once: Necromys lasiurus, Akodon montensis, Sooretamys angouya, Oecomys catherine, Oxymycterus judex, Euryzygomatomys spinosus and Trinomys iheringi. There were higher peaks for diversity and species richness during the winter (dry) months, probably due to higher food availability. The marsupials had a seasonal reproduction with highest population sizes at the end of the rainy seasons. Nectomys squamipes reproduced mostly during rainy periods. Akodon cursor reproduced predominantly in the winter with the highest population peaks occurring during this season. The analysis of the population dynamics of the rodent species indicated that no species behaved as an agricultural pest, probably due to the heterogeneous landscape of high rotativity of vegetable cultivation. Rodent populations were more susceptible to the removal procedure than marsupial ones.Keywords: marsupials, rodents, diversity, long-term study. Populações de pequenos mamíferos em um agro-ecossistema no domínio da Mata Atlântica, sudeste do Brasil ResumoEste estudo reporta dois anos da dinâmica populacional e reprodução de uma comunidade de pequenos mamíferos através de método de remoção. O estudo foi realizado numa área rural na Mata Atlântica, em Sumidouro, Rio de Janeiro, Brasil. Os tamanhos populacionais, a estrutura etária e a reprodução foram estudados para as quatro espécies mais comuns na área de estudo. A diversidade total foi de 1,67 e variou entre 0,8 e 1,67. A riqueza de espécies foi de 13, considerando-se o estudo todo. As espécies mais abundantes foram os roedores Nectomys squamipes (n = 133), Akodon cursor (n = 74) e Oligoryzomys nigripes (n = 25) e os marsupiais Didelphis aurita (n = 58) e Philander frenatus (n = 50 comportou como praga agrícola, provavelmente devido ao ambiente heterogêneo de alta rotatividade de cultivos de legumes. As populações dos roedores foram mais suscetíveis ao procedimento de remoção do que as dos marsupiais.Palavras-chave: marsupiais, roedores, diversidade, estudo de longo prazo.

show abstract

“…Leishmania sp. was also detected by PCR in three rodent species, Rattus rattus, Thrichomys apereoides, and Oryzomys subflavus in Brazil (Oliveira et al 2005), and Rattus norvegicus in Greece (Papadogiannakis et al 2010). In Spain, Lepus granatensis were reported to be infected with L. infantum, and they were capable of infecting sandflies as confirmed by xenodiagnosis (Molina et al 2012).…”

Section: Discussionmentioning

confidence: 89%

“…The role of these species as reservoirs is unclear, but some species are susceptible to clinical development of the disease (Luppi et al 2008, Malta et al 2010. Furthermore, L. infantum has been detected in other groups of wild mammals that are present in urban areas in Brazil, including rodents (Oliveira et al 2005) and marsupials (Schallig et al 2007, Santiago et al, 2007 that may also function as potential reservoirs. Leishmaniasis affecting zoo and wildlife has been recently reviewed (Souza et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

et al. 2014

View full text Add to dashboard Cite

Pesq. Vet. Bras. 34(12):1243-1246, dezembro 2014 1243 RESUMO.-[Diagnóstico de infecção por Leishmania infantum pela reação em cadeia da polimerase em mamíferos silvestres.] A leishmaniose visceral é uma doença infecciosa crônica de mamíferos causada, no Brasil, pelo protozoário Leishmania infantum (sinonímia: Leishmania chagasi) e transmitida pelo flebótomo Lutzomyia longipalpis. Trata-se de uma zoonose endêmica em muitas regiões do Brasil, inclusive em Belo Horizonte, Minas Gerais. Em centros urbanos, leishmaniose visceral acomete principalmente o cão doméstico. Entretanto, L. infantum já foi diagnosticada em outras espécies, incluindo canídeos e primatas de cativeiro em zoológicos. Este estudo buscou avaliar a presença do DNA deste agente em animais de cativeiro e de vida livre da Fundação Zoobotânica de Belo Horizonte através da reação em cadeia da polimerase. Foram analisadas oitenta e uma amostras de sangue oriundas de primatas, carnívoros, ruminantes, edentatos, marsupial e herbívoro de estômago simples. Três primatas Alouatta guariba (bugio marrom) e dois canídeos Speothos venaticus (cachorro--do-mato-vinagre), foram positivos, demonstrando a importância do controle da leishmaniose em áreas endêmicas com a finalidade de conservar a fauna silvestre mantida em cativeiro.

show abstract

PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil

Cited by 97 publications

References 30 publications

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Serological survey for Leishmania sp. infection in wild animals from the municipality of Maringá, Paraná state, Brazil

Small mammal populations of an agroecosystem in the Atlantic Forest domain, southeastern Brazil

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

Contact Info

Product

Resources

About