PCR-based diagnosis, molecular characterization and detection of atypical strains of avian<i>Chlamydia psittaci</i>in companion and wild birds

Madani, Seyed Ahmad; Peighambari, Seyed Mostafa

doi:10.1080/03079457.2012.757288

Cited by 44 publications

(45 citation statements)

References 51 publications

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“…In agreement with the conclusion of Madani & Peighambari [30] who found no stastistically significant seasonal differences in the occurrence of avian chlamydiosis, our results show that the seropositivity of Chlamydia in parrots sampled in spring was not statistically different to that sampled in summer (P > 0·05). Chlamydia can be resistant to temperature variations from ∼8°C in spring to ∼25°C in summer in north China, and the statistically similar seropositivity in parrots in different cities suggests that the pathogen could be mainly transmitted by the direct contact route in birds, which is little influenced by environmental change.…”

Section: Discussionsupporting

confidence: 95%

Seroprevalence and genotype of Chlamydia in pet parrots in China

Zhang

Zhou

et al. 2014

Epidemiol. Infect.

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Parrots are one of the most popular pet birds in China, and can harbour Chlamydia which has significance for human and animal health. We investigated, by indirect haemagglutination assay, the seroprevalence of Chlamydia infection in four species of parrots, namely budgerigars (Melopsittacus undulatus), lovebirds (Agapornis sp.), cockatiels (Nymphicus hollandicus) and Alexandrine parakeets (Psittacula eupatria) that were collected from Weifang and Beijing cities, North China and explored the association between potential risk factors and chlamydial seropositivity. We further determined the genotype of Chlamydia in 21 fresh faecal samples based on the ompA sequence by reconstruction of phylogenetic relationships. Of the 311 parrots examined, 35·37% (95% confidence interval 30·06-40·68) were seropositive, and species, gender, age, season and geographical location were identified as risk factors. Two PCR-positive samples represented Chlamydia psittaci genotype A. The occurrence of C. psittaci genotype A in the droppings of two pet parrots in China suggests potential environmental contamination with Chlamydiaceae and may raise a public health concern.

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Section: Discussionsupporting

confidence: 95%

Seroprevalence and genotype of Chlamydia in pet parrots in China

Zhang

Zhou

et al. 2014

Epidemiol. Infect.

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“…psittaci as the predominant chlamydial agent in cormorants and mallards. Interestingly, many studies report the detection of atypical or non-typable Chlamydiaceae in wild birds [8–9, 16, 21], however, their further characterization was often hampered by unsuccessful cultivation attempts and, thus, the lack of isolates from the unknown species or genotypes. Therefore, the aim of our study was not solely the collection of prevalence data for Chlamydiaceae in wild birds in ten out of sixteen Polish districts (voivodeships), but also the isolation of chlamydial agents to enable their in-depth molecular characterization as well as further investigations into epidemiology, host preference, pathogenicity and zoonotic potential.…”

Section: Introductionmentioning

confidence: 99%

Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

et al. 2017

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Wild birds are considered as a reservoir for avian chlamydiosis posing a potential infectious threat to domestic poultry and humans. Analysis of 894 cloacal or fecal swabs from free-living birds in Poland revealed an overall Chlamydiaceae prevalence of 14.8% (n = 132) with the highest prevalence noted in Anatidae (19.7%) and Corvidae (13.4%). Further testing conducted with species-specific real-time PCR showed that 65 samples (49.2%) were positive for C. psittaci whereas only one was positive for C. avium. To classify the non-identified chlamydial agents and to genotype the C. psittaci and C. avium-positive samples, specimens were subjected to ompA-PCR and sequencing (n = 83). The ompA-based NJ dendrogram revealed that only 23 out of 83 sequences were assigned to C. psittaci, in particular to four clades representing the previously described C. psittaci genotypes B, C, Mat116 and 1V. Whereas the 59 remaining sequences were assigned to two new clades named G1 and G2, each one including sequences recently obtained from chlamydiae detected in Swedish wetland birds. G1 (18 samples from Anatidae and Rallidae) grouped closely together with genotype 1V and in relative proximity to several C. abortus isolates, and G2 (41 samples from Anatidae and Corvidae) grouped closely to C. psittaci strains of the classical ABE cluster, Matt116 and M56. Finally, deep molecular analysis of four representative isolates of genotypes 1V, G1 and G2 based on 16S rRNA, IGS and partial 23S rRNA sequences as well as MLST clearly classify these isolates within the C. abortus species. Consequently, we propose an expansion of the C. abortus species to include not only the classical isolates of mammalian origin, but also avian isolates so far referred to as atypical C. psittaci or C. psittaci/C. abortus intermediates.

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“…Several chlamydia-specific proteins, which are the source of diversity among chlamydial genomes, have now been identified on the inclusion membrane: the group of polymorphic membrane proteins (pmps), inclusion member protein A ( inc A), and outer membrane protein ( omp A) ( 23 , 33 ). Until recently, there were nine omp A genotypes described in C. psittaci (A–F, E/B, M56, and WC), along with a number of provisional genotypes (YP84, R54, 6N, CPX0308, I, and J) representing strains which are untypable so far ( 15 , 24 ). Before the new emerging chlamydial agent – C. gallinacea – was described, C. psittaci was considered to be the dominant Chlamydia species in poultry.…”

Section: Introductionmentioning

confidence: 99%

Poultry in Poland as Chlamydiaceae carrier

Szymańska-Czerwińska

Mitura

Zaręba

et al. 2017

Journal of Veterinary Research

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IntroductionThe study was conducted to investigate the prevalence and genetic diversity of Chlamydia spp. in poultry in Poland and estimate possible transmission to humans.Material and MethodsMolecular diagnostic methods followed by sequencing and strain isolation were used on cloacal/faecal swabs collected from 182 apparently healthy poultry flocks including chickens, turkeys, ducks, and geese. Serum samples obtained from people exposed (study group) and non-exposed (control group) to birds were tested by complement fixation test to acquire data on Chlamydia spp. antibody level.ResultsOverall, 15.9% of the tested flocks were Chlamydiaceae-positive and three Chlamydia spp. were identified. Predominant chlamydial agent found was C. gallinacea occurring in 65.5% of all positive poultry flocks and in 73.0% of positive chicken flocks. The sequences from four chicken flocks were assigned to C. abortus, whereas C. psittaci was confirmed in one duck and one goose flock. The analysis of ompA variable domains revealed at least nine genetic variants of C. gallinacea. Chlamydial antibodies were detected in 19.2% of human serum samples in the study group in comparison with 10.8% in the controls.ConclusionThe obtained results confirm that chlamydiae are common among chicken flocks in Poland with C. gallinacea as a dominant species. Moreover, the presence of C. abortus in chickens is reported here for the first time. Further investigation should focus on possible zoonotic transmission of C. gallinacea and C. abortus as well as potential pathogenic effects on birds’ health and poultry production.

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PCR-based diagnosis, molecular characterization and detection of atypical strains of avianChlamydia psittaciin companion and wild birds

Cited by 44 publications

References 51 publications

Seroprevalence and genotype of Chlamydia in pet parrots in China

Seroprevalence and genotype of Chlamydia in pet parrots in China

Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

Poultry in Poland as Chlamydiaceae carrier

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