1999
DOI: 10.1007/s001220051156
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PCR-based method for identifying the S-genotypes of Japanese pear cultivars

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Cited by 143 publications
(100 citation statements)
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“…A 6-FAM labeled FTQQYQ primer (Ishimizu et al 1999) and a newly designed non-labeled primer (5 0 -RYgtgcatgaaaatctatg-3 0 ) were used to amplify a 254-or 262-bp fragment of the S 4 -or S 5 -RNase gene, respectively (Fig. 2).…”
Section: Determination Of S-rnase and Microsatellite Genotypesmentioning
confidence: 99%
“…A 6-FAM labeled FTQQYQ primer (Ishimizu et al 1999) and a newly designed non-labeled primer (5 0 -RYgtgcatgaaaatctatg-3 0 ) were used to amplify a 254-or 262-bp fragment of the S 4 -or S 5 -RNase gene, respectively (Fig. 2).…”
Section: Determination Of S-rnase and Microsatellite Genotypesmentioning
confidence: 99%
“…Esse sistema apresenta três tipos de interação de compatibilidade: totalmente incompatível, quando ambos os alelos são comuns; parcialmente compatível em que apenas um alelo é diferente; e totalmente compatível, quando os quatro alelos são diferentes e a fecundação ocorre plenamente (KAUFMANN et al, 1992;NEWBI-GIN et al, 1993;LEWIS, 1994;SAKURAI, 2000;BRUCKNER,1994), Métodos que permitam avaliar antecipadamente a compatibilidade genética de alelos-S entre cultivares são de grande interesse, na medida em que permitem selecionar precocemente cultivares totalmente compatíveis, do ponto de vista genético-reprodutivo (MOTA;OLIVEIRA, 2005). Muito embora não permita substituir por completo os ensaios de polinização controlada a campo, a avaliação molecular dos alelos-S torna o processo de seleção de uma polinizadora mais eficiente (BROOTHAERTS et al, 1995;ISHIMIZU et al, 1999;YAEGAKI et al, 2001).…”
Section: Introductionunclassified
“…The HV region was thought to interact with the pollen S gene product(s) to recognize self and non-self pollen (Matsuura et al, 2001). One intron located within the HV region also showed considerable diversification in terms of sequence and length among the S-RNases (Ishimizu et al, 1999).…”
Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning
confidence: 99%
“…On the basis of the variations in the HV region and the intron, the cleaved amplified polymorphic sequence (CAPS) (S 1 to S 7 ) system for discriminating S 1 -to S 7 -haplotypes was developed as a rapid method to identify the S-genotypes of Japanese pear cultivars (Ishimizu et al, 1999). In this system, S-RNase fragments including the intron are amplified from genomic DNA by polymerase chain reaction (PCR) with the consensus primers "FTQQYQ" (5'-TTTACGCAGCAATATCAG-3') and "anti-IIWPNV" [5'-AC(A/G)TTCGGCCAAA (Fig.…”
Section: ) Development Of S-genotyping Systems Using the Pistil S Genementioning
confidence: 99%
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