PCR-based methods to the diagnosis of imported malaria

Berry, Antoine; Benoit‐Vical, Françoise; Fabre, Richard; Cassaing, Sophie; Magnaval, Jean-François

doi:10.1051/parasite/2008153484

Cited by 62 publications

(76 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymerase chain reaction (PCR) offers a good alternative to microscopy in terms of sensitivity and specificity and could be used as a confirmatory tool for microscopy identification of malaria parasites (11,12). Molecular methods, such as single-step PCR (13), loopmediated isothermal amplification (LAMP) assay (14), nested PCR (15,16), multiplex PCR (17,18), multiplex real-time PCR (19), and real time PCR (20)(21)(22)(23) basically use conventional PCR techniques to detect and identify malaria parasites.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of new multiplex PCR primers for the identification ofPlasmodium species found in Sabah, Malaysia

et al. 2016

View full text Add to dashboard Cite

Background/aim: Malaria is a major public health problem, especially in the Southeast Asia region, caused by 5 species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi). The aim of this study was to compare parasite species identification methods using the new multiplex polymerase chain reaction (PCR) against nested PCR and microscopy.Materials and methods: Blood samples on filter papers were subject to conventional PCR methods using primers designed by us in multiplex PCR and previously designed primers of nested PCR. Both sets of results were compared with microscopic identification.Results: Of the 129 samples identified as malaria-positive by microscopy, 15 samples were positive for P. falciparum, 14 for P. vivax, 6 for P. knowlesi, 72 for P. malariae, and 2 for mixed infection of P. falciparum/P. malariae. Both multiplex and nested PCR identified 12 P. falciparum single infections. For P. vivax, 9 were identified by multiplex and 12 by nested PCR. For 72 P. malariae cases, multiplex PCR identified 58 as P. knowlesi and 10 as P. malariae compared to nested PCR, which identified 59 as P. knowlesi and 7 as P. malariae. Conclusion:Multiplex PCR could be used as alternative molecular diagnosis for the identification of all Plasmodium species as it requires a shorter time to screen a large number of samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of new multiplex PCR primers for the identification ofPlasmodium species found in Sabah, Malaysia

et al. 2016

View full text Add to dashboard Cite

show abstract

“…PCR is a more sensitive and specific method of diagnosis than microscopy. 22,23 The low prevalence of congenital malaria observed in this study could also be because of the design (cross-sectional); hence, single sets of blood samples were only collected within the first 24 hours of birth. Other studies that followed-up babies for longer periods of time after birth reported higher prevalence of congenital malaria.…”

Section: Discussionmentioning

confidence: 99%

Congenital Malaria in Calabar, Nigeria: The Molecular Perspective

Oduwole

Gc²,

Odey³

et al. 2011

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) has been shown to be more sensitive in detecting low-level parasitemia than conventional blood film microscopy. We estimated the prevalence of congenital malaria using nested PCR amplification of the small subunit 18S RNA gene to detect low-level parasitemia and identify Plasmodium species in 204 mother–neonate pairs. Cord-blood parasitemia was detected in four babies by PCR, giving a prevalence of 2.0%. The newborns of primidgravidae were more susceptible to congenital malaria than those of multigravidae (P < 0.0001). There was a strong correlation between placental malaria and congenital malaria (odds ratio = 10.1, 95% confidence interval = 1.3–76.1, P = 0.0487). We conclude that the prevalence of congenital malaria in Calabar detected by PCR is lower than has been reported in this environment through microscopy.

show abstract

“…Although the qPCR method requires specific material and is more expensive than mi-Prevalence of P. falciparum and P. vivax in Pará State croscopy and conventional PCR (Berry et al, 2005), it presents advantages of rapidity, lower contamination, and better standardization and can be used for routine testing, particularly for the study of populations in endemic areas in which patients may be asymptomatic (Mens et al, 2007;Boonma et al, 2007;Gama et al, 2007;Veron et al, 2009) and in patients who have negative results on routine methods but are strongly suspected of having malaria (Bourgeois et al, 2010). Thus, qPCR assay based on the detection of mitochondrial cytochrome c oxidase genes may be the method of choice for specific situations, including detection in low-parasitized individuals.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Pará State, Brazil, determined by amplification of mtDNA using a real-time PCR assay

Souza

Carvalho²,

Amaral³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probes. Since the first study published in 2001 at least 17 assays have been developed, most of them using SSUrRNA as the target gene. We used qPCR to detect Plasmodium falciparum and P. vivax by amplification of mtDNA; this technique was evaluated on whole-blood samples from people living in areas of malaria transmission in the Brazilian Amazon region located in the area of inclusion of highway BR-163 (Cuiabá-Santarém) in Pará State: São Luiz do Tapajós, a municipal district of Itaituba (N = 74); Três Boeiras, a municipal district of Trairão (N = 134), and São Raimundo, a municipal district of Aveiro (N = 62). The results from the real-time PCR-based method were compared to conventional microscopy and to an established mtDNA-PCR assay. The qPCR (mtDNA) method was 16-19 times more efficient than the conventional PCR (mtDNA) and microscopy for detecting plasmodial infections.

show abstract

PCR-based methods to the diagnosis of imported malaria

Cited by 62 publications

References 42 publications

Evaluation of new multiplex PCR primers for the identification ofPlasmodium species found in Sabah, Malaysia

Evaluation of new multiplex PCR primers for the identification ofPlasmodium species found in Sabah, Malaysia

Congenital Malaria in Calabar, Nigeria: The Molecular Perspective

Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Pará State, Brazil, determined by amplification of mtDNA using a real-time PCR assay

Contact Info

Product

Resources

About