PCR Based Molecular Diagnostic Assays for Brucellosis: A Review

Kumar, Vinay; Bansal, Nitish; Nanda, Trilok; Kumar, Aman; Kumari, Rajni; Maan, Sushila

doi:10.20546/ijcmas.2019.802.312

Cited by 3 publications

(1 citation statement)

References 74 publications

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“…Thus for omp31 gene of B. melitensis , the use of nested PCR’s may be explored. However, the omp2 gene of B. melitensis was detected and so it can be targeted for species specific detection of B. melitensis from field samples such as genital swabs (38, 39).…”

Section: Results and Dicsussionmentioning

confidence: 99%

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

Madan¹,

Kumaresan

Rekha³

et al. 2022

Preprint

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Caprine brucellosis due to Brucella melitensis is an important zoonotic disease. The present study was carried out to address the lack of a comprehensive study on the status of caprine brucellosis in Puducherry, India using serological and molecular tests in goats and to assess the seroprevalence in human risk groups of the aforementioned region to ascertain the public health significance of the disease. Seroprevalence in 120 goats was found to be zero, 3.33% and 18.33% by Rose Bengal agglutination Test (RBT), Standard Tube Agglutination Test (STAT) and Immunoglobulin G Indirect Enzyme Linked Immune Sorbant Assay (IgG iELISA) respectively. Of the 120 goat genital swabs screened, while conventional polymerase chain reaction (PCR) detected genus specific 16S rRNA and Brucella melitensis specific omp2 genes in 17.50% and 5.00% of samples respectively, the OMP31TaqMan® real time PCR with a positive detection of 40.00% was both the most sensitive and specific for detection of Brucella melitensis. The study provides insight into the optimization of diagnostic tests following cluster wise sampling for brucellosis in goats. The strain of B. melitensis in Puducherry was found to be Biovar 3 based upon suggestive results of Restriction Fragment Length Polymorphism (RFLP) of omp2 gene product. Seroprevalence by IgG iELISA was 33.33 % in 30 samples from human subjects. Serological evidence of caprine brucellosis in goats and human subjects and molecular detection of B.melitensis in Puducherry, India warrants regular screening, surveillance and reporting of disease in goats and human risk groups.

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Section: Results and Dicsussionmentioning

confidence: 99%

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

Madan¹,

Kumaresan

Rekha³

et al. 2022

Preprint

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Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis

et al. 2019

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Epidemiological Investigation of Animal Brucellosis in Domestic Ruminants in Greece from 2015 to 2022 and Genetic Characterization of Prevalent Strains

Emmanouil,

Vourvidis,

Kyrma

et al. 2024

Pathogens

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Brucellosis is one of the most important zoonotic diseases in Greece, causing a significant burden on both human and animal vitality as well as economic loss. The present study was conducted from 2015 to 2022 on 711,415 serum samples by determining the seroepidemiology of Brucellosis among livestock in 24 geographical areas in Greece using the Rose Bengal Test (RBT) and the complement fixation test (CFT) and further performing genetic analysis of Brucella spp. by species-specific real-time PCR and MLVA Brucella analysis. A total of 3086 serum samples from goats, sheep, and cattle showed positive results using the RBT and CFT, and only strongly positive samples (n = 800) were preserved in the Βlood Bank of the Veterinary Laboratory of Brucellosis. From these, 212 sera samples were randomly selected for molecular and genetic analysis. The results indicated that the incidence rate of Brucellosis is higher in cattle herds in comparison with other animal species. Overall, 48 samples tested positive by real-time PCR, of which forty-seven of them were B. abortus and one was B. melitensis. Genetic analysis of two B. abortus samples revealed a common pattern, indicating two Bruce04, two Bruce18, four Bruce07, two Bruce09, three Bruce16, and four Bruce30 for both samples, which, interestingly, were not identical with the known genotypes in the public MLVA Brucella database. Our findings substantiate that animal Brucellosis remains a health issue in Greece, with a stable but apparent incidence rate, and further investigation is needed to fully characterize the newly identified Brucella strains in Greece.

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PCR Based Molecular Diagnostic Assays for Brucellosis: A Review

Cited by 3 publications

References 74 publications

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis

Epidemiological Investigation of Animal Brucellosis in Domestic Ruminants in Greece from 2015 to 2022 and Genetic Characterization of Prevalent Strains

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