PCR detection and sequencing of parasite ITS-rDNA gene from reservoirs host of zoonotic cutaneous leishmaniasis in central Iran

Parvizi, Parviz; Moradi, Ghasem; Akbari, Ghasem; Farahmand, Mahin; Ready, P. D.; Piazak, N; Assmar, Mehdi; Amirkhani, Aref

doi:10.1007/s00436-008-1220-0

Cited by 7 publications

(10 citation statements)

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“…In this study, nested PCR of ITS1-5.8S rDNA gene proved to be more sensitive for Leishmania species identification. This proved to be consistent with a number of previously obtained results ( Ajouad et al, 2013 , El Tai et al, 2000 , El Tai et al, 2001 , Spanakos et al, 2008 , Schallig and Oskam, 2002 , Parvizi et al, 2008 , Es-Sette et al, 2014 ). All of the cases studied were caused by L. tropica , a species endemic to the Aleppo region in Syria ( Saroufim et al, 2014 ) thus supporting the import of this species into Lebanon from across the Syrian borders.…”

Section: Discussionsupporting

confidence: 93%

Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

Salloum

Khalifeh

Tokajian

2016

Parasite Epidemiology and Control

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Leishmania is a parasitic protozoan with more than two-dozen species causing the disease leishmaniasis. It is transmitted to humans through the bite of an infected female phlebotomine sand-fly vector. In the past two years the incidence of leishmaniasis has been drastically increasing in Lebanon. This was in parallel with the deterioration of the security in Syria forcing thousands to flee and seek shelter in poorly maintained refugee camps and collective shelters. Cutaneous leishmaniasis (CL) is now considered a public health problem, but its epidemiology has not been fully elucidated. To our knowledge, this is the first study comparing two different molecular methods for the detection and identification of Leishmania tropica in Lebanon.Two molecular typing methods of 39 FFPE Leishmania isolates were used: the ITS1-PCR RFLP and the nested ITS1-5.8S rDNA gene amplification followed by sequencing and phylogenetic analysis. The efficiency of these two techniques in Leishmania identification was compared and the phylogenetic relationships among these isolates were illustrated based on the neighbor-joining (NJ) method. The results were statistically correlated with the parasitic index (PI). The DNA storage in formalin-fixed paraffin embedded (FFPE) tissues was assessed as well. The parasites identified were all L. tropica as determined by both techniques. ITS1-5.8S rDNA gene based typing proved to be more sensitive in the detection of parasites (positive in 69.2% of the isolates) as opposed to the ITS1-PCR RFLP method that was successful in identifying L. tropica in only 43.6% of the isolates. Sequencing and phylogenetic analysis revealed high levels of heterogeneity. A statistically significant correlation was observed between PI and the results of the nested ITS1-5.8S rDNA gene PCR. Genotyping at the species level is essential for monitoring the relative frequency of CL in the Mediterranean area that is correlated to three different Leishmania species (Leishmania infantum, Leishmania major and L. tropica), each characterized by distinct epidemiological features. The obtained results highlight the need to find a universally accepted diagnostic tool for Leishmania typing.

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Section: Discussionsupporting

confidence: 93%

Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

Salloum

Khalifeh

Tokajian

2016

Parasite Epidemiology and Control

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“…Iran and in some other countries [6,16,19,22]. In most of the earlier studies conducted in Iran, only L. major was isolated from great gerbils and characterized using isoenzyme or DNA-based molecular techniques [9,10,21]. In a study, which was conducted in the same area, only L. major was reported [18].…”

Section: Discussionmentioning

confidence: 96%

Dynamics of Leishmania infection rates in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of zoonotic cutaneous leishmaniasis in Iran

Akhavan

Yaghoobi–Ershadi

Khamesipour

et al. 2010

Bull. Soc. Pathol. Exot.

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Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is a great public health problem in the Old World. Leishmania major is widely distributed in populations of rodents in arid and savannah regions. In this study, seasonal variation of natural infection with Leishmania parasites in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of ZCL in Iran was monitored. The study was conducted from October 2007 to October 2008 in the central part of the country. Nested polymerase chain reaction (PCR) assay was used for the detection and identification of Leishmania parasites, and the results were confirmed by PCR-restriction fragment length polymorphism (RFLP). The results showed that Leishmania infection rate was 55.8% (29 out of 52 gerbils) using nested PCR. The highest and lowest Leishmania infection rates were observed in fall and summer, respectively. Gerbils that were found to be infected only with L. major were 5.8%, and that with Leishmania turanica were 23.1%. A mixed natural infection was seen in the rodents with L. major and L. turanica (21.2%), with L. major and L. gerbilli (1.9%), and with all the three species (3.9%). Leishmania major infection alone was seen in fall and winter whereas mixed infection of L. major and L. turanica was observed in all seasons except in summer. Leishmania turanica infection was observed throughout the year. It is concluded that L. major, L. gerbilli, and L. turanica circulate in the population of R. opimus in central part of Iran. Leishmania major infection is usually accompanied by L. turanica in naturally infected gerbils with the highest rate in fall. It is recommended that the role of L. turanica in the epidemiology and transmission of ZCL should be reconsidered.

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“…In the detection of leishmanial infection in rodents, the investigation of biopsy/necropsy samples by PCR is more sensitive than the investigation of similar samples as smears or NO. AND (%) WITH PCR-POSITIVE: Foot/feet only 0 (0) 3 (3.1) 4 (19.0) 0 (0) Ear(s) only 0 (0) 4 (6.3) 3 (14.3) 0 (0) Liver only 0 (0) 2 (3.1) 0 (0) 0 (0) Spleen only 0 (0) 4 (6.3) 0 (0) 0 (0) Foot/feet and ear(s) only 0 (0) 2 (3.1) 2 (9.5) 0 (0) Foot/feet and liver only 0 (0) 0 (0) 0 (0) 0 (0) Foot/feet and spleen only 0 (0) 0 (0) 0 (0) 0 (0) Ear(s) and liver only 0 (0) 4 (6.3) 0 (0) 0 (0) Ear(s) and spleen only 0 (0) 1 (1.6) 0 (0) 0 (0) Spleen and liver only 0 (0) 4 (6.3) 0 (0) 0 (0) Ear(s), foot/feet and liver only 2 (67) 2 (3.1) 0 (0) 0 (0) Ear(s), spleen and liver only 0 (0) 6 (9.4) 0 (0) 0 (0) Ear(s), foot/feet and spleen only 1 (33) 3 (4.7) 0 (0) 0 (0) Ear(s), foot/feet, spleen and liver 0 (0) 1 (4.8) 0 (0) 0 (0) by culture (Nicolas et al, 2002;Oliveira et al, 2005;Parvizi et al, 2008). Even though the samples investigated in the present study had been formalin-fixed, the overall prevalence of L. major detected (52.8%) was markedly higher than the value (16.2%) estimated, using microscopy and culture, for rodents caught elsewhere in southern Iran (Motazedian et al, 2006;Mehrabani et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The PCR-based detection ofLeishmania majorinMus musculusand other rodents caught in southern Iran: a guide to sample selection

Parhizkari¹,

Motazedian²,

Asqari³

et al. 2011

Annals of Tropical Medicine & Parasitology

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In recent years, rural cutaneous leishmaniasis (CL) has become one of the most challenging public-health problems in several countries. Rodents play an important role as the 'reservoir' hosts of the parasite (Leishmania major) that causes the vast majority of the CL found in rural Iran. The present study deals with the role of rodents in the epidemiology of CL in Fars province, in the south of Iran, where the incidence of the disease has doubled over the last decade. Samples, of skin, foot pads, ears, livers and spleens, were collected from 89 rodents (three Meriones persicus, one Me. libycus, 64 Tatera indica and 21 Mus musculus), that had been caught in an endemic area, killed, and fixed in formalin [5% (w/v) formaldehyde]. When each sample was checked for L. major DNA by PCR, at least one sample from each of three (100%) of the Me. persicus, 35 (54.7%) of the T. indica and nine (42.9%) of the Mu. musculus was found to contain L. major DNA. Only 60% of the ear samples, 56% of the footpad samples, 38% of the liver samples and 38% of the spleen samples from the PCR-positive rodents were, however, found PCR-positive. Four of the rodents (two T. indica and two Mu. musculus) were each found to have PCR-positive ear and footpad samples but PCR-negative liver and spleen samples. Only four of the rodents (all Reprint requests to: M. H. Motazedian.

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PCR detection and sequencing of parasite ITS-rDNA gene from reservoirs host of zoonotic cutaneous leishmaniasis in central Iran

Cited by 7 publications

References 0 publications

Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

Dynamics of Leishmania infection rates in Rhombomys opimus (Rodentia: Gerbillinae) population of an endemic focus of zoonotic cutaneous leishmaniasis in Iran

The PCR-based detection ofLeishmania majorinMus musculusand other rodents caught in southern Iran: a guide to sample selection

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