PCR Detection of Bovine Papilloma Virus DNA in Superficial Swabs and Scrapings from Equine Sarcoids

Martens, Ann; Moor, Antoine De; Ducatelle, Richard

doi:10.1053/tvjl.2000.0524

Cited by 89 publications

(64 citation statements)

References 17 publications

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“…The identification of BPV13 in the sarcoids examined in this study gives rise to the hypothesis that a fraction of PV isolates previously diagnosed from sarcoids worldwide by PCRs using BPV1/2 E5-specific primers might have been misclassified as BPV2, especially as direct sequencing of numerous amplicons was not performed (10,15,(29)(30)(31). To avoid the misidentification of PV types, the use of general PCRs with broad-spectrum primers and sequencing is recommended.…”

Section: Discussionmentioning

confidence: 99%

Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

et al. 2013

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bEquine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.

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Section: Discussionmentioning

confidence: 99%

Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

et al. 2013

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“…Superficial skin swabs collected for the purpose of viral-DNA detection were taken from 73 WBBs examined during field monitoring activities carried out between 2005 and 2007 using sterile 0.9% NaCl-soaked cotton-tipped swabs following the methods of Martens et al and Antonsson et al (2,31). The number and origin of WBBs examined during this study and the number of individuals with clinical evidence of the papillomatosis and carcinomatosis syndrome are presented in Table 1.…”

Section: Methodsmentioning

confidence: 99%

A Novel Virus Detected in Papillomas and Carcinomas of the Endangered Western Barred Bandicoot ( Perameles bougainville ) Exhibits Genomic Features of both the Papillomaviridae and Polyomaviridae

Woolford

Rector

Ranst

et al. 2007

J Virol

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Conservation efforts to prevent the extinction of the endangered western barred bandicoot (Perameles bougainville) are currently hindered by a progressively debilitating cutaneous and mucocutaneous papillomatosis and carcinomatosis syndrome observed in captive and wild populations. In this study, we detected a novel virus, designated the bandicoot papillomatosis carcinomatosis virus type 1 (BPCV1), in lesional tissue from affected western barred bandicoots using multiply primed rolling-circle amplification and PCR with the cutaneotropic papillomavirus primer pairs FAP59/FAP64 and AR-L1F8/AR-L1R9. Sequencing of the BPCV1 genome revealed a novel prototype virus exhibiting genomic properties of both the Papillomaviridae and the Polyomaviridae. Papillomaviral properties included a large genome size (ϳ7.3 kb) and the presence of open reading frames (ORFs) encoding canonical L1 and L2 structural proteins. The genomic organization in which structural and nonstructural proteins were encoded on different strands of the double-stranded genome and the presence of ORFs encoding the nonstructural proteins large T and small t antigens were, on the other hand, typical polyomaviral features. BPCV1 may represent the first member of a novel virus family, descended from a common ancestor of the papillomaviruses and polyomaviruses recognized today. Alternatively, it may represent the product of ancient recombination between members of these two virus families. The discovery of this virus could have implications for the current taxonomic classification of Papillomaviridae and Polyomaviridae and can provide further insight into the evolution of these ancient virus families.

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“…This was corroborated further by an investigation of sarcoids in donkeys . However, more recently, aberrant perinuclear localization of p53 has been demonstrated in 44 % of equine sarcoid lesions (Martens et al, 2001b), suggesting that mutational independent inactivation of p53 occurs commonly in sarcoids; the significance of these finding remains to be elucidated.…”

Section: Other Factors Involved In Sarcoid Developmentmentioning

confidence: 99%

Association of bovine papillomavirus with the equine sarcoid

Chambers

Ellsmore

O’Brien

et al. 2003

Journal of General Virology

187

180

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The equine sarcoid, a locally aggressive, fibroblastic skin tumour, is the most common dermatological neoplasm reported in horses; there is no consistently effective therapy. It is widely accepted that bovine papillomavirus (BPV) types 1 and 2 are associated with the pathogenesis of sarcoid disease. Most sarcoids appear to contain detectable viral DNA and RNA and are also known to express the BPV types 1 and 2 major transforming protein, E5, but appear not to produce infectious virions. While the mode of transmission of infection has not been elucidated, viral gene expression, in particular of E5, may contribute to virus persistence and disease pathogenesis by downregulating MHC class I expression. Here, the pathology and epidemiology of the sarcoid and its association with BPV is reviewed; the transforming functions of the BPV oncoproteins and their possible role in sarcoid pathogenesis are discussed; and the practical implications of BPV infection for diagnostic and therapeutic purposes are considered.

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PCR Detection of Bovine Papilloma Virus DNA in Superficial Swabs and Scrapings from Equine Sarcoids

Cited by 89 publications

References 17 publications

Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

A Novel Virus Detected in Papillomas and Carcinomas of the Endangered Western Barred Bandicoot ( Perameles bougainville ) Exhibits Genomic Features of both the Papillomaviridae and Polyomaviridae

Association of bovine papillomavirus with the equine sarcoid

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